Covalent Immobilization of β-Glucosidase into Mesoporous Silica Nanoparticles from Anhydrous Acetone Enhances Its Catalytic Performance

Sannino, Filomena; Costantini, A.; Ruffo, Francesco; Aronne, Antonio; Venezia, Virginia; Califano, Valeria

doi:10.3390/nano10010108

Cited by 53 publications

(35 citation statements)

References 62 publications

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“…By increasing the temperature up to 70 • C, a cellobiose conversion of 100% is retained; because a 2 h batch-equivalent contact time corresponds to the first full conversion detected at 50 • C, no deactivation occurs up to 70 • C. A further temperature increase causes a drastic performance reduction. These results agree with those reported on powder WSNs under batch reaction conditions [18] showing increased stability of the immobilized enzyme compared to the free one between 50 and 70 • C; this result suggests that neither washcoating onto the monolith walls nor the flow conditions affect the BG thermostability. As stated above, no deactivation occurs between 50 and 70 • C; however, due to the full conversion measured within this temperature range, an eventual activity increase could not have been measured.…”

Section: /T Ksupporting

confidence: 92%

Immobilization of β-Glucosidase over Structured Cordierite Monoliths Washcoated with Wrinkled Silica Nanoparticles

et al. 2020

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The enzymatic conversion of biomass-derived compounds represents a key step in the biorefinery flowsheet, allowing low-temperature high-efficiency reactions. β-Glucosidases are able to hydrolyze cellobiose into glucose. Wrinkled silica nanoparticles (WSNs) were demonstrated to be a good support for the immobilization of β-glucosidases, showing better performance than free enzymes in batch reaction; on the other hand, immobilized enzyme microreactors (IEMs) are receiving significant attention, because small quantities of reagents can be used, and favorable heat and mass transfer can be achieved with respect to conventional batch systems. In this work, we prepared, characterized, and tested structured enzymatic reactor compounds by a honeycomb monolith, a WSN washcoat, and β-glucosidases as the active phase. Powder and structured materials were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), N2 physisorption, thermogravimetric analysis (TGA), and Fourier-transform infrared spectroscopy (FT-IR). Structured catalysts were tested under both batch and continuous flow reaction conditions and compared to powder catalysts (batch reaction). The WSN washcoat was attached well onto the monolith walls, as suggested by the negligible weight loss after ultrasound treatment; the WSNs preserved their shape, porosity, and individual nature when deposited onto the monolith walls. The immobilized enzyme microreactors proved to be very efficient in hydrolysis of cellobiose to glucose, showing a complete conversion under continuous flow reaction at a batch-equivalent contact time equal to 120 min vs. 24 h obtained in the batch experiments. The apparent KM value showed a 20-fold decrease with respect to the batch process, due to the absence of external diffusive transport limitations.

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Section: /T Ksupporting

confidence: 92%

Immobilization of β-Glucosidase over Structured Cordierite Monoliths Washcoated with Wrinkled Silica Nanoparticles

et al. 2020

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“…The FT-IR spectrum of the HA-APTS hybrid precursor showed a marked decrease of the bands in the region between 1400 and 1650 cm −1 , including the COOasymmetric stretching vibration modes [53][54][55]. Furthermore, the spectrum showed a shoulder at around 1560 cm −1 , which can be assigned to the out-of-phase combination mode of the N-H in the plane bend and the C-N stretching vibration of the Amide II band [56,57]. Finally, by comparing the FTIR spectra of the bare HA and HA-APTS precursor, changes in the adsorption band of the OH vibrations in the range of 3500 cm −1 were clearly visible.…”

Section: Peak Position (Cm −1 ) Vibrational Mode ~1100mentioning

confidence: 98%

Tuning Functional Behavior of Humic Acids through Interactions with Stöber Silica Nanoparticles

et al. 2020

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Humic acids (HA) exhibit fascinating multifunctional features, yet degradation phenomena as well as poor stability in aqueous environments strongly limit their use. Inorganic nanoparticles are emerging as a powerful interface for the development of robust HA bio-hybrid materials with enhanced chemical stability and tunable properties. Hybrid organic-inorganic SiO 2 /HA nanostructures were synthesized via an in-situ sol-gel route, exploiting both physical entrapment and chemical coupling. The latter was achieved through amide bond formation between carboxyl groups of HA and the amino group of 3-aminopropyltriethoxysilane (APTS), as confirmed by Fourier-Transform Infrared (FTIR) and Nuclear Magnetic Resonance (NMR) spectroscopy. Monodisperse hybrid nanoparticles about 90 nm in diameter were obtained in both cases, yet Electron Paramagnetic Resonance (EPR) spectroscopy highlighted the different supramolecular organization of HA. The altered HA conformation was reflected in different antioxidant properties of the conjugated nanoparticles that, however, resulted in being higher than for pure HA. Our findings proved the key role of both components in defining the morphology of the final system, as well as the efficacy of the ceramic component in templating the HA supramolecular organization and consequently tuning their functional features, thus defining a green strategy for bio-waste valorization.

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“…In the last decade, fly ash was used as raw materials for obtaining zeolites X (FAU framework type) [8,9], Y (FAU) [10], A (LTA) [8,9,11] or ZSM-5 (MFI) [12]. Furthermore, the synthesis of mesoporous silica from fly ash has also attracted interest due to the resulting material characteristics [13][14][15][16][17][18]. Mesoporous silica materials obtained from fly ash are considered to surpass the limitations of the microporous zeolites in the removal of macromolecule pollutants by adsorption [13].…”

Section: Introductionmentioning

confidence: 99%

Fly Ash, from Recycling to Potential Raw Material for Mesoporous Silica Synthesis

Miricioiu

Niculescu

2020

Nanomaterials

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In order to meet the increasing energy demand and to decrease the dependency on coal, environmentally friendly methods for fly ash utilization are required. In this respect, the priority is to identify the fly ash properties and to consider its potential as raw material in the obtaining of high-value materials. The physico-chemical and structural characteristics of the fly ash coming from various worldwide power plants are briefly presented. The fly ash was sampled from power plants where the combustion of lignite and hard coal in pulverized-fuel boilers (PC) and circulating fluidized bed (CFB) boilers was applied. The fly ash has high silica content. Due to this, the fly ash can be considered a potential raw material for the synthesis of nanoporous materials, such as zeolites or mesoporous silica. The samples with the highest content of SiO2 can be used to obtain mesoporous silica materials, such as MCM-41 or SBA-15. The resulting mesoporous silica can be used for removing/capture of CO2 from emissions or for wastewater treatment. The synthesis of various porous materials using wastes would allow a high level of recycling for a sustainable society with low environmental impact.

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Covalent Immobilization of β-Glucosidase into Mesoporous Silica Nanoparticles from Anhydrous Acetone Enhances Its Catalytic Performance

Cited by 53 publications

References 62 publications

Immobilization of β-Glucosidase over Structured Cordierite Monoliths Washcoated with Wrinkled Silica Nanoparticles

Immobilization of β-Glucosidase over Structured Cordierite Monoliths Washcoated with Wrinkled Silica Nanoparticles

Tuning Functional Behavior of Humic Acids through Interactions with Stöber Silica Nanoparticles

Fly Ash, from Recycling to Potential Raw Material for Mesoporous Silica Synthesis

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