Covalent immobilization of the multienzyme enniatin synthetase

Multifunctional Peptide Synthetases

“…Peptide synthesis can be carried out by incubation of the relevant synthetases with the constituent amino acids and ATP in solution or in immobilized form. 111 In the case of N-methylated peptides Sadenosylmethionine must be added. 112 The immediate precursors forming adenylates have to be known, since some enter the cycle in the D configuration (e.g., D-Ala in cyclosporin), or even have to be hydroxylated in advance (D-4-hydroxyproline in neoviridogrisein).…”

“…Peptide synthesis can be carried out by incubation of the relevant synthetases with the constituent amino acids and ATP in solution or in immobilized form . In the case of N -methylated peptides S -adenosylmethionine must be added .…”

Fungal cyclooligomerdepsipeptides: From classical biochemistry to combinatorial biosynthesis

“…The half lives of the enzyme preparations were found to be diverse, ranging from 12 h at 0 C for the PF1022 synthetase 61 to 50 h at 25 C for the enniatin synthetase. 83 Generally, protease content was a limiting factor, and proteinase inhibitors were found to be essential for stabilization (H. Peeters, Doctoral Thesis, TU Berlin, 1988). Reported catalytic activities range from 2 pkat mg À1 (beauvericin synthetase) and 12 pkat mg À1 (PF1022 synthetase) to 100 pkat mg À1 for the enniatin synthetase.…”