Covalent immobilisation of manganese peroxidases (MnP) from Phanerochaete chrysosporium and Bjerkandera sp. BOS55

Mielgo, I.; Palma, C.; Guisán, José M.; Fernandez-Lafuente, R; Moreira, Marı́a Teresa; Lema, Juan M.

doi:10.1016/s0141-0229(03)00066-8

Cited by 36 publications

(20 citation statements)

References 25 publications

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“…The results indicated that MnP was sufficiently immobilized on carriers and immobilized MnP had a higher thermo stability and resistance to high H 2 O 2 concentrations than free MnP. Mielgo et al [6] compared two strategies for the immobilization of MnP and demonstrated that a higher yield of immobilization and activity were obtained by covalent immobilization than by ionic adsorption. However, the stability and loading of immobilized MnP were still too low for industrial applications [30].…”

Section: Resultsmentioning

confidence: 99%

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Noncovalent immobilization of manganese peroxidases from P. chrysosporium on carbon nanotubes

Wen

2009

Front. Environ. Sci. Eng. China

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Manganese peroxidases (MnP) from Phanerochaete chrysosporium were adsorbed onto multi-walled carbon nanotubes (MWNT). Four different loadings of MnP on MWNTs were investigated, and the maximum enzyme loading of 47.5 µg/mg of MWNTs was obtained in 12 h. The adsorbed MnP showed a catalytic activity of up to 0.1 U/mg of the weight of the system of MnP/MWNTs, with 23% of its original activity retained. The AFM image of the adsorbed enzymes indicated that a layer of MnP covered the surface of the MWNTs and retained its original three-dimensional shape. Amino-based nonspecific interactions may play the dominant role in the adsorption of MnP on MWNTs.

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Section: Resultsmentioning

confidence: 99%

“…BOS55, and Lentinula edodes, has been investigated previously. The nano-assembly of MnP and LiP from P. chrysosporium have been described as biocatalysis in aqueous and non-aqueous media [5][6][7]. Mielgo et al reported that immobilization could improve thermal stability and resistance against denaturing agents such as H 2 O 2 for MnP [6].…”

Section: Introductionmentioning

confidence: 99%

Noncovalent immobilization of manganese peroxidases from P. chrysosporium on carbon nanotubes

Wen

2009

Front. Environ. Sci. Eng. China

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“…Edwards et al (2002) demonstrated that the application of laccase and MnP from T. versicolor immobilized onto polysulfone ultrafiltration membranes could remove aromatic hydrocarbons from a petrochemical industrial effluent. Mielgo et al (2003) studied the immobilization of MnP from P. chrysosporium and Bjerkandera sp. in glutaraldehyde-agarose gels, obtaining a complete immobilization in a very short period (0.5-2 h).…”

Section: Discussionmentioning

confidence: 99%

White-Rot Fungi in Bioremediation

2012

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“…The degradation rate was 96% at pH 4.5 and 78% at pH 8.2 at 30 days. Enzyme immobilization is a usable method for maintaining high percentage activity of an enzyme for long incubation, increases the thermostability of an enzyme, and avoids inactivity because of an imbalance in the chemical content in a fungal culture [27].…”

Section: Bap Degradation Using Free Crude Enzyme and Alginate-bead Immentioning

confidence: 99%

Enhanced Culture in Static Batch Systems for Benzo[a]pyrene Degradation by Bjerkandera Adusta SM46 under Acidic and Saline Conditions

Andriani¹,

Tachibana²,

Itoh³

2017

J-SustaiN

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Biodegradation of high-molecular weight polycyclic aromatic hydrocarbons (PAHs) is biologically difficult to degrade due to its recalcitrant characteristics such as low solubility in water and inhibition effect. In the present study, we used an enhanced culture system (ECS) for Bjerkandera adusta SM46 on benzo[a]pyrene (BaP) degradation under acidic (pH 4.5) and saline conditions (pH 8.2). The ECS using Tween 80 (T-80) and MnSO4 enhanced both the ligninolytic enzyme activities and degradation rate by the fungus. The optimum condition was obtained at a concentration for MnSO4 of 0.4-0.9 mM and for T-80 of 0.45-0.70%, which increased degradation more than 87% at day 30 (pH 4.5). The ECS could also be applied under a saline condition for up to 82% degradation. Mangan peroxidase (MnP) and lignin peroxidase (LiP) showed high activities at pH 4.5 (110.9 and 79.9 U/L, respectively). Moreover, laccase and MnP showed higher activities (75.6 and 130.0 U/L, respectively) at saline pH 8.2 indicating stress condition stimulation. * Corresponding AuthorTel.: +81-89-9469864; E-mail: tatibana@agr.ehime-u.ac Using rice straw also enhanced the BaP degradation rate by the fungus via cell growth promotion. This study suggests that an ECS treatment and rice straw co-substrate system could potentially enhance PAH degradation for a static batch system under both an acidic and a salinealkaline stress condition.

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Covalent immobilisation of manganese peroxidases (MnP) from Phanerochaete chrysosporium and Bjerkandera sp. BOS55

Cited by 36 publications

References 25 publications

Noncovalent immobilization of manganese peroxidases from P. chrysosporium on carbon nanotubes

Noncovalent immobilization of manganese peroxidases from P. chrysosporium on carbon nanotubes

White-Rot Fungi in Bioremediation

Enhanced Culture in Static Batch Systems for Benzo[a]pyrene Degradation by Bjerkandera Adusta SM46 under Acidic and Saline Conditions

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