Covalent conjugation of single-walled carbon nanotube with CYP101 mutant for direct electrocatalysis

Ray, Moumita; Mhaske, Sanjay D.; Haram, Santosh K.; Mazumdar, Shyamalava

doi:10.1016/j.ab.2021.114204

Cited by 6 publications

(1 citation statement)

References 31 publications

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“…As shown in Table 2, the ASNase adsorption onto CXs promoted a slight increase in the I D /I G ratio values, from 1.19 for pristine CXs to 1.35 for the ASNase-CXs composite. This result is indicative of an increase in the structural disorder of CXs after enzyme immobilization, as was already observed during the immobilization of other enzymes onto carbon nanomaterials [47,48].…”

Section: Characterizationsupporting

confidence: 70%

Immobilization and Characterization of L-Asparaginase over Carbon Xerogels

Barros

Cristóvão

Carabineiro

et al. 2022

BioTech

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L-asparaginase (ASNase) is an aminohydrolase currently used in the pharmaceutical and food industries. Enzyme immobilization is an exciting option for both applications, allowing for a more straightforward recovery and increased stability. High surface area and customizable porosity make carbon xerogels (CXs) promising materials for ASNase immobilization. This work describes the influence of contact time, pH, and ASNase concentration on the immobilization yield (IY) and relative recovered activity (RRA) using the Central Composite Design methodology. The most promising results were obtained using CX with an average pore size of 4 nm (CX-4), reaching IY and RRA of 100%. At the optimal conditions (contact time 49 min, pH 6.73, and [ASNase] 0.26 mg·mL−1), the ASNase-CXs biocomposite was characterized and evaluated in terms of kinetic properties and operational, thermal, and pH stabilities. The immobilized ASNase onto CX-4 retained 71% of its original activity after six continuous reaction cycles, showed good thermal stability at 37 °C (RRA of 91% after 90 min), and was able to adapt to both acidic and alkaline environments. Finally, the results indicated a 3.9-fold increase in the immobilized ASNase affinity for the substrate, confirming the potential of CXs as a support for ASNase and as a cost-effective tool for subsequent use in the therapeutic and food sectors.

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Section: Characterizationsupporting

confidence: 70%

Immobilization and Characterization of L-Asparaginase over Carbon Xerogels

Barros

Cristóvão

Carabineiro

et al. 2022

BioTech

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In silico study of substrate chemistry effect on the tethering of engineered antibodies for SARS-CoV-2 detection: Amorphous silica vs gold

Martí

Martín-Martínez

Torras

et al. 2022

Colloids and Surfaces B: Biointerfaces

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The influence of the properties of different solid substrates on the tethering of two antibodies, IgG1-CR3022 and IgG1-S309, which were specifically engineered for the detection of SARS-CoV-2, has been examined at the molecular level using conventional and accelerated Molecular Dynamics (cMD and aMD, respectively). Two surfaces with very different properties and widely used in immunosensors for diagnosis, amorphous silica and the most stable facet of the face-centered cubic gold structure, have been considered. The effects of such surfaces on the structure and orientation of the immobilized antibodies have been determined by quantifying the tilt and hinge angles that describe the orientation and shape of the antibody, respectively, and the dihedrals that measure the relative position of the antibody arms with respect to the surface. Results show that the interactions with amorphous silica, which are mainly electrostatic due to the charged nature of the surface, help to preserve the orientation and structure of the antibodies, especially of the IgG1-CR3022, indicating that the primary sequence of those antibodies also plays some role. Instead, short-range van der Waals interactions with the inert gold surface cause a higher degree tilting and fraying of the antibodies with respect to amorphous silica. The interactions between the antibodies and the surface also affect the correlation among the different angles and dihedrals, which increases with their strength. Overall, results explain why amorphous silica substrates are frequently used to immobilize antibodies in immunosensors.

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A sensitive electrochemical sensor manufactured from multi-wall carbon nanotubes-polyethylenimine nanocomposite for malachite green detection

Luo

2022

Journal of Alloys and Compounds

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Covalent conjugation of single-walled carbon nanotube with CYP101 mutant for direct electrocatalysis

Cited by 6 publications

References 31 publications

Immobilization and Characterization of L-Asparaginase over Carbon Xerogels

Immobilization and Characterization of L-Asparaginase over Carbon Xerogels

In silico study of substrate chemistry effect on the tethering of engineered antibodies for SARS-CoV-2 detection: Amorphous silica vs gold

A sensitive electrochemical sensor manufactured from multi-wall carbon nanotubes-polyethylenimine nanocomposite for malachite green detection

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