Covalent attachment of shape-restricted DNA molecules on amine-functionalized Si(111) surface

Nanoparticle layer deposition for highly controlled multilayer formation based on high-coverage monolayers of nanoparticles

“…This observation and previous experience in surface modification of solid substrates with buckyballs [41], biomolecules [42, 43], and nanoparticles [42] can be expanded for surface functionalization of silica nanoparticles and their deposition on a gold-coated substrate in a layer-by-layer manner. Again, it should be emphasized that this is not a process of self-assembly but rather a chemically-driven self-limiting process.…”

Click Chemistry‐Based Functionalization on Non‐Oxidized Silicon Substrates

“…In many cases, it is necessary to use a protected precursor during the grafting process, which is then deprotected after linking to the silicon substrate to obtain the desired functionality (Scheme 2). [29][30][31][32] A notable exception is the olefins bearing carboxylic acid (ÀCOOH) functionality, which did not require protection as they appeared to react primarily through the alkene end. [33][34][35][36] Though only small-to-negligible indications of upside-down attachment were reported, there are still complications due to strong physisorption of carboxylic acids through COOH···HOOC hydrogen bonding.…”

“…The challenge to directly prepare alkynyl-terminated monolayers on non-oxidized silicon is that 1-alkyne can also attach onto the Si À H substrate through the mechanism similar to alkene, forming À C=C À Si bonds at the organic/Si interface. [30][31][32] This process is energetically more favorable than the reaction with alkenes. [33,54,55] As a result, if an alkeneyne is simply used for the grafting, competitive attachment of double bonds and triple bonds would be expected.…”

Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates

“…The immobilization of ssDNA on glass and silicon substrates is usually performed on surfaces functionalized with silanes, e.g., epoxy-or amine-terminated silane, on which ssDNA can be covalently coupled either directly or by employing appropriate cross-linkers [6][7][8][9][10][11][12]. Concerning other types of substrates such as gold electrode surfaces, ssDNA probes are usually immobilized either through direct bonding of thiol-modified oligonucleotides [13,14], or via bonding of 5 -end modified ssDNA using bifunctional reagents (e.g., sulfosuccinimidyl-4-(Nmaleimidomethyl)cyclohexane-1-carboxylate or N-succinimidyl-S-acetylthiopropionate) [15].…”