Covalent attachment of microbial lipase onto microporous styrene–divinylbenzene copolymer by means of polyglutaraldehyde

Functional Porous Polymers by Emulsion Templating: Recent Advances

“…[ 107 ] There was a noticeable increase in the stability of the covalently immobilised lipase in comparison to lipase adsorbed on a styrene/DVB polyHIPE support. After reusing the support with adsorbed lipase just fi ve times, there was a complete loss of enzyme activity.…”

Response surface methodology for optimal immobilization of Aspergillus niger ATCC 1015 lipase by adsorption method

“…Immobilized lipases on solid support allow recoverability and reuse which can lead to a significant reduction in operational costs of industrial processes (Mateo et al 2007, Liu & Chang 2008, Pahujani et al 2008). The hydrophobic binding of immobilized lipases by adsorption has proved successful due to the enzyme affinity for water/oil interfaces (Deng et al 2004, Dizge et al 2008). Experimental statistical techniques such as RSM, provides an effective alternative to the conventional approach in many biotechnological processes thus revealing the correlations between the factors and responses as well as the optimum level of each factor employed (Bussamara et al 2012).…”

Synthesis and characterization of a novel fish scale-immobilized chitosan adsorbent—Preliminary features of dichlorophenol sorption by solution calorimetry

“…Glutaraldehyde (GA) possesses unique characteristics that render it one of the most effective collagen cross linking reagents. It can react with several functional groups of collagens, such as amine, thiol, phenol, and imidazole by several means such as aldol condensation or Michael-type addition [7,17]. However, most of solutions of GA is usually polymeric and contain ␣,␤-unsaturated aldehydes (PGA) that are able to form rings by loss of water molecules by aldol condensation [7].…”