Coupling of δ13C and δ15N to understand soil organic matter sources and C and N cycling under different land-uses and management: a review and data analysis

Park, Hyun-Jin; Baek, Nuri; Lim, Sang-Sun; Jeong, Young-Jae; Seo, Bo-Seong; Kwak, Jin‐Hyeob; Lee, Sang-Mo; Yun, Seok-In; Kim, Han-Yong; Arshad, Muhammad; Choi, Woo‐Jung

doi:10.1007/s00374-022-01668-3

Cited by 14 publications

(33 citation statements)

References 78 publications

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“…For each mass spectrum, the peak heights for the monoisotopic, 13 C 1 , and 15 N 1 isotopologue features were extracted for caffeine [M + H] + (m/z 195), MRFA [M + 2H] 2+ (m/z 262), and MRFA [M + H] 1+ (m/z 524). Figure 1 shows the measured ratio of the 13 C and 15 N A + 1 isotopologue abundances relative to the monoisotopic peak for MRFA 1+, normalized per the number of atoms of each element in the molecule.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…For both MRFA 1+ and caffeine, a 13 C/ 12 C isotope ratio precision (relative standard error) below 1‰ is achieved in about 5 × 10 6 effective counts, which, due to differences in stoichiometry and isotope ratios, requires 2460 and 3751 spectra, respectively, or about 68 and 104 min of measurement time. For 15 N, 1‰ precision is only achieved after 16,303 and 22,509 spectra, or 453 and 626 min, respectively, due to significantly lower natural abundance, fewer atoms in the molecule, and thus lower signal-to-noise ratio (S/N) in each spectrum.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Stable isotope measurement of organic compounds can reveal information about ambient conditions of formation, source substrates and formation mechanisms, and forensic discrimination of a molecule’s provenance. Demonstrated applications for isotope analysis include interpretation of paleoclimates, − forensic linkage to geographic origin, authenticity, , industrial process, , identifying doping in athletic competitors, dietary trends, paleoaltimetry, ecology, or biogeochemical pathways and stability . Distinguishing subtle shifts in isotopic content required the development of instrumentation and methods capable of high precision and accuracy of stable isotope measurements.…”

Section: Introductionmentioning

confidence: 99%

“…For final measurements, three standards of caffeine of known isotopic ratios were purchased from the US Geological Survey (USGS). Samples are listed in Table 1, along with their reported δ 13 C and δ 15 N. 28 For direct infusion measurements, USGS caffeine samples were prepared at 10 μg/mL in 1:1 acetonitrile:water with 0.1% formic acid. The calibration sample was used as supplied.…”

Section: ■ Introductionmentioning

confidence: 99%

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Natural Abundance Isotope Ratio Measurements of Organic Molecules Using 21 T FTICR MS

Kew,

Boiteau,

Eiler

et al. 2023

Anal. Chem.

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Subtle variations in stable isotope ratios at natural abundance are challenging to measure but can yield critical insights into biological, physical, and geochemical processes. Wellestablished methods, particularly multicollector, gas-source, or plasma isotope ratio mass spectrometry, are the gold standard for stable isotope measurement, but inherent limitations in these approaches make them ill-suited to determining site-specific and multiply substituted isotopic abundances of all but a few compounds or to characterizing larger intact molecules. Fourier transform mass spectrometry, namely, Orbitrap mass spectrometry, has recently demonstrated the ability to measure natural abundance isotope ratios with chemically informative accuracy and precision. Here, we report the first use of Fourier transform ion cyclotron resonance mass spectrometry for the accurate (<1‰) and precise (<1‰ standard error) simultaneous determination of δ 13 C and δ 15 N in caffeine isotopologues and provide a discussion of the critical instrumental parameters necessary to make such measurements. We further report the ability to make these measurements with online liquid chromatography, expanding the ability of this technique to explore mixtures in the future.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Natural Abundance Isotope Ratio Measurements of Organic Molecules Using 21 T FTICR MS

Kew,

Boiteau,

Eiler

et al. 2023

Anal. Chem.

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show abstract

“…The response of SON in different-sized aggregates to land-use change is different, for example, there is a quicker response in macro-aggregates than in micro-aggregates ( Lan, Hu & Fu, 2020 ). Thus, soil aggregates combined with 15 N natural abundance can provide a clearer understanding of soil N dynamics under land-use change including secondary succession after agricultural land abandonment ( Park et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Alterations of ecosystem nitrogen status following agricultural land abandonment in the Karst Critical Zone Observatory (KCZO), Southwest China

Liu

Han

2023

PeerJ

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Background Secondary succession after agricultural land abandonment generally affects nitrogen (N) cycle processes and ecosystem N status. However, changes in soil N availability and NO3– loss potential following secondary succession are not well understood in karst ecosystems. Methods In the Karst Critical Zone Observatory (KCZO) of Southwest China, croplands, shrub-grass lands, and secondary forest lands were selected to represent the three stages of secondary succession after agricultural land abandonment by using a space-for-time substitution approach. The contents and 15N natural abundance (δ15N) of leaves, soils, and different-sized aggregates at the three stages of secondary succession were analyzed. The δ15N compositions of soil organic nitrogen (SON) in aggregates and soil to plant 15N enrichment factor (EF = δ15Nleaf −δ15Nsoil), combined with soil inorganic N contents and δ15N compositions were used to indicate the alterations of soil N availability and NO3–loss potential following secondary succession. Results Leaf N content and SON content significantly increased following secondary succession, indicating N accumulation in the soil and plant. The δ15N values of SON also significantly decreased, mainly affected by plant δ15N composition and N mineralization. SON content in macro-aggregates and soil NH4+ content significantly increased while δ15N values of NH4+ decreased, implying increases in SON stabilization and improved soil N availability following secondary succession. Leaf δ15N values, the EF values, and the (NO3–-N)/(NH4+-N) ratio gradually decreased, indicating reduced NO3– loss following secondary succession. Conclusions Soil N availability improves and NO3– leaching loss reduces following secondary succession after agricultural land abandonment in the KCZO.

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Stable isotopes for the study of soil C and N under global change

et al. 2023

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Coupling of δ13C and δ15N to understand soil organic matter sources and C and N cycling under different land-uses and management: a review and data analysis

Cited by 14 publications

References 78 publications

Natural Abundance Isotope Ratio Measurements of Organic Molecules Using 21 T FTICR MS

Natural Abundance Isotope Ratio Measurements of Organic Molecules Using 21 T FTICR MS

Alterations of ecosystem nitrogen status following agricultural land abandonment in the Karst Critical Zone Observatory (KCZO), Southwest China

Stable isotopes for the study of soil C and N under global change

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