Coupling of bacterial nitrification with denitrification and anammox supports N removal in intertidal sediments (Arcachon Bay, France)

Fernandes, Sheryl Oliveira; Javanaud, Cedric; Michotey, Valérie; Guasco, Sophie; Anschutz, Pierre; Bonin, Patricia

doi:10.1016/j.ecss.2015.10.009

Cited by 37 publications

(14 citation statements)

References 74 publications

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“…Figure 3a shows the NRE of the A/SAD/HD reactor during the organic change stage. Without the addition of organic matter (phase I), the average NRE achieved in this reactor gradually reached 97.3% on the last five days of phase I, which is comparable to those of other A/SAD systems [14,19]. During phases II-IV, sodium acetate was fed to the reactor to promote heterotrophic denitrification.…”

Section: High-throughput Sequencing and Bioinformatic Analysissupporting

confidence: 60%

Feasibility of Adjusting the S2O32−/NO3− Ratio to Adapt to Dynamic Influents in Coupled Anammox and Denitrification Systems

Hou

Cheng

Wang

et al. 2020

IJERPH

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In this study, anammox, sulfur-based autotrophic denitrification, and heterotrophic denitrification (A/SAD/HD) were coupled in an expanded granular sludge bed (EGSB) reactor to explore the feasibility of enhancing denitrification performance by adjusting the S2O32−/NO3− (S/N) ratio to accommodate dynamic influents. The results indicated that the optimal influent conditions occurred when the conversion efficiency of ammonium (CEA) was 55%, the S/N ratio was 1.24, and the chemical oxygen demand (COD) was 50 mg/L, which resulted in a total nitrogen removal efficiency (NRE) of 95.0% ± 0.5%. The S/N ratio regulation strategy was feasible when the influent COD concentration was less than 100 mg/L and the CEA was between 57% and 63%. Characterization by 16S rRNA sequencing showed that Candidatus Jettenia might have contributed the most to anammox, while Thiobacillus and Denitratisoma were the dominant taxa related to denitrification. The findings of this study provide insights into the effects of CEA and COD on the performance of the A/SAD/HD system and the feasibility of the S/N ratio regulation strategy.

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Section: High-throughput Sequencing and Bioinformatic Analysissupporting

confidence: 60%

Feasibility of Adjusting the S2O32−/NO3− Ratio to Adapt to Dynamic Influents in Coupled Anammox and Denitrification Systems

Hou

Cheng

Wang

et al. 2020

IJERPH

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“…Under in situ conditions, nitrite fueling the anammox process could originate from nitrate reduction by anammox bacteria or other nitrate reducers but also from incomplete nitrification. Oxygen concentrations of 0–2.2 μmol L −1 in the groundwater of suboxic well H53 may provide conditions supportive of a coupling between aerobic and anaerobic ammonium oxidation as described from marine oxygen minimum zones (Lam et al, 2007 , 2009 ; Lam and Kuypers, 2011 ) or recently also from intertidal sediments (Fernandes et al, 2016 ). Detection of transcripts of archaeal and bacterial amoA genes in the groundwater of well H53 suggested ongoing aerobic ammonia oxidation at suboxic conditions, albeit at a much lower transcriptional activity compared to oxic well H41, where nitrification rates of 10.4–14.4 nmol NO x L −1 d −1 were detected in a previous study (Opitz et al, 2014 ).…”

Section: Discussionmentioning

confidence: 80%

Nitrogen Loss from Pristine Carbonate-Rock Aquifers of the Hainich Critical Zone Exploratory (Germany) Is Primarily Driven by Chemolithoautotrophic Anammox Processes

et al. 2017

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Despite the high relevance of anaerobic ammonium oxidation (anammox) for nitrogen loss from marine systems, its relative importance compared to denitrification has less been studied in freshwater ecosystems, and our knowledge is especially scarce for groundwater. Surprisingly, phospholipid fatty acids (PLFA)-based studies identified zones with potentially active anammox bacteria within two superimposed pristine limestone aquifer assemblages of the Hainich Critical Zone Exploratory (CZE; Germany). We found anammox to contribute an estimated 83% to total nitrogen loss in suboxic groundwaters of these aquifer assemblages at rates of 3.5–4.7 nmol L−1 d−1, presumably favored over denitrification by low organic carbon availability. Transcript abundances of hzsA genes encoding hydrazine synthase exceeded nirS and nirK transcript abundances encoding denitrifier nitrite reductase by up to two orders of magnitude, providing further support of a predominance of anammox. Anammox bacteria, dominated by groups closely related to Cand. Brocadia fulgida, constituted up to 10.6% of the groundwater microbial community and were ubiquitously present across the two aquifer assemblages with indication of active anammox bacteria even in the presence of 103 μmol L−1 oxygen. Co-occurrence of hzsA and amoA gene transcripts encoding ammonia mono-oxygenase suggested coupling between aerobic and anaerobic ammonium oxidation under suboxic conditions. These results clearly demonstrate the relevance of anammox as a key process driving nitrogen loss from oligotrophic groundwater environments, which might further be enhanced through coupling with incomplete nitrification.

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“…Nitrite influenced both archaeal and bacterial communities, while fungi were independent of both nitrite and nitrate. Due to the low availability of nitrate at the burrow wall (which increased away from the burrow) at every depth, we hypothesise that nitrate is rapidly consumed by the burrow wall microbiome and/or readily released into burrow water and flushed out at high tide 43 .…”

Section: Discussionmentioning

confidence: 99%

The role of fungi in heterogeneous sediment microbial networks

Booth

Fusi

Marasco

et al. 2019

Sci Rep

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While prokaryote community diversity and function have been extensively studied in soils and sediments, the functional role of fungi, despite their huge diversity, is widely unexplored. Several studies have, nonetheless, revealed the importance of fungi in provisioning services to prokaryote communities. Here, we hypothesise that the fungal community plays a key role in coordinating entire microbial communities by controlling the structure of functional networks in sediment. We selected a sediment environment with high niche diversity due to prevalent macrofaunal bioturbation, namely intertidal mangrove sediment, and explored the assembly of bacteria, archaea and fungi in different sediment niches, which we characterised by biogeochemical analysis, around the burrow of a herbivorous crab. We detected a high level of heterogeneity in sediment biogeochemical conditions, and diverse niches harboured distinct communities of bacteria, fungi and archaea. Saprotrophic fungi were a pivotal component of microbial networks throughout and we invariably found fungi to act as keystone species in all the examined niches and possibly acting synergistically with other environmental variables to determine the overall microbial community structure. In consideration of the importance of microbial-based nutrient cycling on overall sediment ecosystem functioning, we underline that the fungal microbiome and its role in the functional interactome cannot be overlooked.

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Coupling of bacterial nitrification with denitrification and anammox supports N removal in intertidal sediments (Arcachon Bay, France)

Cited by 37 publications

References 74 publications

Feasibility of Adjusting the S2O32−/NO3− Ratio to Adapt to Dynamic Influents in Coupled Anammox and Denitrification Systems

Feasibility of Adjusting the S2O32−/NO3− Ratio to Adapt to Dynamic Influents in Coupled Anammox and Denitrification Systems

Nitrogen Loss from Pristine Carbonate-Rock Aquifers of the Hainich Critical Zone Exploratory (Germany) Is Primarily Driven by Chemolithoautotrophic Anammox Processes

The role of fungi in heterogeneous sediment microbial networks

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