Countrywide Spread of CTX-M-3 Extended-Spectrum β-Lactamase-Producing Microorganisms of the Family
            <i>Enterobacteriaceae</i>
            in Poland

Baraniak, Anna; Fiett, Janusz; Sulikowska, Agnieszka; Hryniewicz, Waleria; Gniadkowski, Marek

doi:10.1128/aac.46.1.151-159.2002

Cited by 109 publications

(97 citation statements)

References 43 publications

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“…At the same time they were equally susceptible to cefoxitin (MICs, 2 to 4 g/ml), suggesting that altered permeability was not likely contributing to the increased resistance. This observation supports the findings of Baraniak et al, who found that resistance phenotype conferred by the CTX-M-3 enzyme may be variable, possibly reflecting the fluctuations in the level of its expression (1). Although in the study of Yu et al high-level resistance to cefepime was strongly associated with the CTX-M-type ESBLs (43), our data indicate that the MICs of cefepime alone are not always predictive of a particular ESBL type.…”

Section: Resultssupporting

confidence: 55%

“…Initially identified in Poland (19), it was later found in France (16,18), Greece (23), and Taiwan (42,44). The countrywide spread of CTX-M-3 ␤-lactamaseproducing organisms was recently reported in Poland, where this type of ESBL was found in six species of the family Enterobacteriaceae, including K. pneumoniae (1). As shown by PCR-RFLP analysis, the majority (71.4%) of CTX-M ␤-lactamases produced by E. coli isolates were also distributed to the CTX-M-1 cluster.…”

Section: Resultsmentioning

confidence: 99%

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Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Edelstein

Pimkin

Palagin

et al. 2003

Antimicrob Agents Chemother

320

181

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A total of 904 consecutive nosocomial isolates of Escherichia coli and Klebsiella pneumoniae collected from 28 Russian hospitals were screened for production of extended-spectrum ␤-lactamases (ESBLs). The ESBL phenotype was detected in 78 (15.8%) E. coli and 248 (60.8%) K. pneumoniae isolates. One hundred fifteen isolates carried the genes for CTX-M-type ␤-lactamases, which, as shown by PCR-restriction fragment length polymorphism analysis, were distributed into the two genetic groups of CTX-M-1 (93%)-and CTX-M-2 (7%)-related enzymes. Isolates producing the enzymes of the first group were found in 20 hospitals from geographically distant regions of the country and were characterized by considerable diversity of genetic types, as was demonstrated by enterobacterial repetitive consensus PCR typing. Within this group the CTX-M-3 and the CTX-M-15 ␤-lactamases were identified. In contrast, the enzymes of the CTX-M-2 group (namely, CTX-M-5) were detected only in eight clonally related E. coli isolates from a single hospital. Notably, the levels of resistance to ceftazidime were remarkably variable among the CTX-M producers. This study provides further evidence of the global dissemination of CTX-M type ESBLs and emphasizes the need for their epidemiological monitoring.During the past decade extended-spectrum ␤-lactamases (ESBLs) of the CTX-M type emerged in many countries of the world. The first organisms producing ␤-lactamases of this type were identified both as single and epidemic clinical isolates in very distant geographic regions (Germany and France and Argentina) in the early 1990s (3, 5, 7). More recently, a rapid increase in the proportion of multiple CTX-M variants to the TEM-and SHV-derived ESBLs has been reported in many hospitals in Spain (9,12,15,35) (14), and Korea (28). Furthermore, CTX-M ␤-lactamases, mainly types CTX-M-2 and CTX-M-3, were found to be widespread or even predominant ESBL types in several countries, including Argentina (31, 34; M. Galas, F. Pasteran, R. Melano, A. Petroni, G. Lopez, A. Corso, A. Rossi, et al., Abstr. 38th Intersci. Conf. Antimicrob. Agents Chemother., abstr. E-109, 1998), Japan (41), and Poland (1).Currently the CTX-M family includes more than 20 ␤-lactamases, which may be grouped on the basis of sequence similarity into four distinct clusters (subtypes) epitomized by CTX-M-1, CTX-M-2, CTX-M-8, and CTX-M-9 (26, 37; http: //www.lahey.org/studies/webt.htm). Within each cluster there is a high degree of bla CTX-M gene sequence identity (Ͼ95%), although members of different subtypes share only 70 to 77% similarity at the nucleotide level. The recent finding of Kluyvera ascorbata species-specific ␤-lactamases (KluA) which share 98.6 to 100% identity with CTX-M-2 and CTX-M-5 and analysis of DNA sequences adjacent to the KluA-and CTX-Mcoding genes provide strong evidence of the direct evolution of the CTX-M-2 cluster from the chromosomal enzyme of K. ascorbata (20,36). Likewise, the chromosomal KLUG-1 enzyme of Kluyvera georgiana was found to be the most-probable progenitor of ...

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Section: Resultssupporting

confidence: 55%

Section: Resultsmentioning

confidence: 99%

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Edelstein

Pimkin

Palagin

et al. 2003

Antimicrob Agents Chemother

320

181

View full text Add to dashboard Cite

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“…Several outbreaks caused by CTX-M-type ESBLs have been reported (Baraniak et al, 2002;Boyd et al, 2004;Woodford et al, 2004;Yan et al, 2000). In the UK, a recent study identified an epidemic CTX-M-15-producing Escher- ; 6, Y19 (CTX-M-9, group 9); 7, Y15 (CTX-M-14, group 9); 8, TRA (CTX-M-21, group 9); 9, F2 (CTX-M-2, group 2); 10, TLR (CTX-M-20, group 2); 11, WH208537 (SHV-12); 12, WH758373 (SHV-11 and TEM-1); 13, water negative control.…”

Section: Evaluation Of Multiplex Pcr With Clinical Isolatesmentioning

confidence: 99%

Rapid and simple detection of bla CTX−M genes by multiplex PCR assay

Ensor

Gossain

et al. 2005

Journal of Medical Microbiology

108

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A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla CTX-M genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla CTX-M genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.

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“…AJ416343) have also been reported to associate with bla CTX-M-2 and bla CTX-M-9 genes (Saladin et al, 2002;Eckert et al, 2006). CTX-M enzymes have been found predominantly in members of the Enterobacteriaceae, most prevalently in E. coli, K. pneumoniae and Salmonella enterica serovar Typhimurium in South America (Radice et al, 2002;Villegas et al, 2004) and Europe (Baraniak et al, 2002;Batchelor et al, 2005;Livermore & Hawkey, 2005), although they are rarely isolated in Japan (Yagi et al, 2000). The spread of CTX-M enzyme-producing E. coli among community-and hospital-based patients has also been reported (Woodford et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

Nakano

Abe

et al. 2012

Journal of Medical Microbiology

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Proteus mirabilis is a common cause of urinary tract infection. Wild-type P. mirabilis strains are usually susceptible to penicillins and cephalosporins, but occurrences of P. mirabilis producing extended-spectrum b-lactamases (ESBLs) have been recently reported. Here, we surveyed the prevalence of cefotaxime resistance among P. mirabilis strains at seven different hospitals in Kanagawa Prefecture, Japan, and investigated their molecular epidemiology to explain the mechanism of their spread. The prevalence of cefotaxime resistance among P. mirabilis increased annually, from 10.1 % in 1998 to 23.1 % in 2003, and increased drastically in 2004, exceeding 40 %. We collected 105 consecutive and non-duplicate cefotaxime-resistant P. mirabilis isolates (MIC 16 to .256 mg ml "1 ) from these hospitals from June 2004 to May 2005 and characterized their profile. PCR and sequence analysis revealed that all resistant strains produced exclusively CTX-M-2 b-lactamase. PFGE analysis identified 47 banding patterns with 83 % or greater similarity. These results indicated that a regional outbreak of P. mirabilis producing CTX-M-2 blactamase has occurred in Japan and suggest that the epidemic spread occurred within and across hospitals and communities by extended clonal strains. Plasmid analysis revealed that 44.8 % of plasmids harboured by bla CTX-M-2 isolates had common profiles, encoding ISEcp1, IS26 and Int1, and belonged to incompatibility group T. Spread of the resistant isolates in Japan resulted from dissemination of narrow-host-range plasmids of the IncT group encoding bla CTX-M-2 . These findings indicate the rapidly developing problem of treating the species to prevent dissemination of ESBL producers.

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Countrywide Spread of CTX-M-3 Extended-Spectrum β-Lactamase-Producing Microorganisms of the Family Enterobacteriaceae in Poland

Abstract: Eighty-four clinical isolates of the family

Cited by 109 publications

References 43 publications

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Rapid and simple detection of bla CTX−M genes by multiplex PCR assay

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

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