Countrywide Molecular Survey of Methicillin-Resistant
            <i>Staphylococcus aureus</i>
            Strains in Poland

Luczak-Kadlubowska, Agnieszka; Sulikowska, Agnieszka; Empel, Joanna; Piasecka, Anna; Orczykowska, Monika; Kozińska, Aleksandra; Hryniewicz, Waleria

doi:10.1128/jcm.00869-08

Cited by 27 publications

(23 citation statements)

References 48 publications

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“…(Rhine Hesse), and ST228-I (Southern Germany). CC8, which was the most prevalent clonal complex in our study, has been also prevalent in other countries, with especially high prevalence of ST239-III (EMRSA-1 [17]. With the exception of the considerable dominance of CC8 (78%), our study results are consistent with the data from other European countries.…”

Section: Resultssupporting

confidence: 94%

Molecular and Phenotypic Characteristics of Methicillin-resistant Staphylococcus aureus Strains Isolated From Hospitalized Patients in Transplantation Wards

Młynarczyk

Szymanek-Majchrzak

Grzybowska

et al. 2014

Transplantation Proceedings

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Section: Resultssupporting

confidence: 94%

Molecular and Phenotypic Characteristics of Methicillin-resistant Staphylococcus aureus Strains Isolated From Hospitalized Patients in Transplantation Wards

Młynarczyk

Szymanek-Majchrzak

Grzybowska

et al. 2014

Transplantation Proceedings

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“…These isolates belong to the dominant Taiwan C-MRSA clones (CC59:SCCmec V:pvl-positive and spa CC t437). Since CC59:SCCmec V isolates have now been reported in other countries (7,10,13,15,19) and since other C-MRSA lineages have also been found to have low-level ␤-lactam resistance (23,28,30), the findings and implications of our study may be relevant to predominant C-MRSA lineages on other continents. Our results indicate that as more clinical laboratories switch to using automated susceptibility testing instruments for determining antimicrobial susceptibility, there is a need for careful evaluation of the performance of these methods for the detection of mecA-positive S. aureus with a low-level oxacillin resistance to prevent their further spread in health care and community settings.…”

Section: Discussionmentioning

confidence: 63%

“…This clone also accounted for 13.1% of C-MRSA isolated from Chinese children in multiple hospitals in China (10). In Europe, the ST59:SCCmec V MRSA has also been detected in the Netherlands (15), while SCCmec V carrying ST338, a single-locus variant of ST59, has also been found in Poland (19). These reports indicate the potential for this clone to spread in other countries.…”

mentioning

confidence: 83%

mecA -Positive Staphylococcus aureus with Low-Level Oxacillin MIC in Taiwan

et al. 2012

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Although the presence of mecA is the genotypic determinant of methicillin-resistant Staphylococcus aureus (MRSA), certain MRSA strains, especially community-associated MRSA (C-MRSA), can display an oxacillin MIC in the Clinical and Laboratory Standards Institute susceptible breakpoint range (<2 g/ml). Among 91 and 180 isolates thought to be methicillin-susceptible S. aureus (MSSA) with oxacillin MICs of 2 and 1 g/ml as determined by the Sensititre broth microdilution test initially, 52 (57.1%) and 6 (3.3%), respectively, were mecA positive. These mecA-positive low-oxacillin-MIC isolates belong to the dominant Taiwan C-MRSA clone (clonal complex [CC] 59), 56 of which carried SCCmec type V and were pvl positive, and 43 of which belonged to spa CC t437. All 271 isolates were retested by Sensititre, as well as by Vitek II and disk diffusion (DD). Based on the oxacillin results, the sensitivities of the Sensititre, Vitek II, and DD methods were 48.3% (28/58), 46.6% (27/58), and 89.6% (52/ 58), respectively. Although cefoxitin was better at detecting these isolates, 12.1, 10.4, and 5.2% of these isolates were still misidentified as MSSA by Sensititre, Vitek II, and DD, respectively. These results highlight the difficulty in the accurate identification of MRSA with borderline oxacillin MICs in the CC59:SCCmec V clone, which likely has contributed to its spread in the health care and community settings. Since this clone has now been detected in other countries, and since other C-MRSA lineages have also been found to have low-level ␤-lactam resistance, the findings of the present study may be relevant to other regions. Further studies are warranted to determine the extent and clinical impact of such misidentification.

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“…Three isolates belonged to the clone ST241-SCC mec III. Two belonged to the ST247-SCC mec I (Iberian) clone, which is one of predominant clones in Poland [37]. Two other isolates belonged to ST239-SCC mec III (Hungarian) clone, which is predominant in Turkey [38].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of methicillin-resistant Panton-valentine leukocidin positive staphylococcus aureus clones disseminating in Tunisian hospitals and in the community

et al. 2013

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BackgroundThe spread of MRSA strains at hospitals as well as in the community are of great concern worldwide. We characterized the MRSA clones isolated at Tunisian hospitals and in the community by comparing them to those isolated in other countries.ResultsWe characterized 69 MRSA strains isolated from two Tunisian university hospitals between the years 2004-2008. Twenty-two of 28 (79%) community-associated MRSA (CA-MRSA) strains and 21 of 41 (51%) healthcare-associated MRSA (HA-MRSA) strains were PVL-positive. The PVL-positive strains belonged to predicted founder group (FG) 80 in MLST and carried either type IVc SCCmec or nontypeable SCCmec that harbours the class B mec gene complex. In contrast, very diverse clones were identified in PVL-negative strains: three FGs (5, 15, and 22) for HA-MRSA strains and four FGs (5, 15, 45, and 80) for CA-MRSA strains; and these strains carried the SCCmec element of either type I, III, IVc or was nontypeable. The nucleotide sequencing of phi7401PVL lysogenized in a CA-MRSA strain JCSC7401, revealed that the phage was highly homologous to phiSA2mw, with nucleotide identities of more than 95%. Furthermore, all PVL positive strains were found to carry the same PVL phage, since these strains were positive in two PCR studies, identifying gene linkage between lukS and mtp (major tail protein) and the lysogeny region, both of which are in common with phi7401PVL and phiSa2mw.ConclusionsOur experiments suggest that FG80 S. aureus strains have changed to be more virulent by acquiring phi7401PVL, and to be resistant to β-lactams by acquiring SCCmec elements. These novel clones might have disseminated in the Tunisian community as well as at the Tunisian hospitals by taking over existing MRSA clones.

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Countrywide Molecular Survey of Methicillin-Resistant Staphylococcus aureus Strains in Poland

Cited by 27 publications

References 48 publications

Molecular and Phenotypic Characteristics of Methicillin-resistant Staphylococcus aureus Strains Isolated From Hospitalized Patients in Transplantation Wards

Molecular and Phenotypic Characteristics of Methicillin-resistant Staphylococcus aureus Strains Isolated From Hospitalized Patients in Transplantation Wards

mecA -Positive Staphylococcus aureus with Low-Level Oxacillin MIC in Taiwan

Molecular characterization of methicillin-resistant Panton-valentine leukocidin positive staphylococcus aureus clones disseminating in Tunisian hospitals and in the community

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