Counting bacteria on a microfluidic chip

Song, Yongxin; Zhang, Hongpeng; Chon, Chan Hee; Chen, Shu; Pan, Xinxiang; Li, Dongqing

doi:10.1016/j.aca.2010.09.035

Cited by 44 publications

(34 citation statements)

References 27 publications

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“…Therefore, our counting technique has same accuracy as that of manual counting. The accuracy of this technique is excellent as verified with independent methods in conventional labs such as by flow cytometer [46]. Also, the particle adhesion on the channel wall has no impact on the detection, as long as the sensing gate is not blocked.…”

Section: Resultsmentioning

confidence: 83%

Nanoparticle detection by microfluidic Resistive Pulse Sensor with a submicron sensing gate and dual detecting channels-two stage differential amplifier

Song

Zhang

Chon

et al. 2011

Sensors and Actuators B: Chemical

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Section: Resultsmentioning

confidence: 83%

Nanoparticle detection by microfluidic Resistive Pulse Sensor with a submicron sensing gate and dual detecting channels-two stage differential amplifier

Song

Zhang

Chon

et al. 2011

Sensors and Actuators B: Chemical

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“…Multiple microfluidic-based methods, including particle counter (Bernabini et al, 2011;Joo et al, 2010;Song et al, 2010), biosensor (Mannoora et al, 2010;Varshney and Li, 2009), and on-chip microbial culture-based methods (Bouguelia et al, 2013;GomezSjoberg et al, 2005), have been utilized for the quantification of bacteria. Among the various label-free signal transduction platforms, impedance biosensors hold promise due to their simple instrumentation, ease of device assembly, and adaptability to multiplexed lab-on-a-chip applications (Boehm et al, 2007; Mannoora et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

A microfluidic device for label-free detection of Escherichia coli in drinking water using positive dielectrophoretic focusing, capturing, and impedance measurement

Kim

Jung

Kim³

et al. 2015

Biosensors and Bioelectronics

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“…The requirement for big equipment is a major bottleneck for realtime and rapid detection of bacteria. Microfluidic chip technology could incorporate chemical and biological functional units (Jung et al 2011) with additional benefits of small volume, high integration and easy operation (Song et al 2010;Fronczek et al 2013), which meets the requirements for rapid and high integration detection in the fields of food safety and environmental testing. Existing microfluidic chip methods for bacteria detection mostly rely on molecular biology methods (Baek et al 2015;Chang et al 2015), light detection Zuo et al 2013), or electrochemical detection .…”

Section: Introductionmentioning

confidence: 99%

An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli

Wang

Liu

et al. 2017

Biomed Microdevices

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An integrated microsystem device with matched interdigitated microelectrode chip was fabricated for enrichment and detection of Escherichia coli O157:H7. The microsystem has integrated with positive dielectrophoresis (pDEP) enrichment and in situ impedance detection, whose total volume is only 3.0 × 10 m, and could provide impedance testing voltages of 0 ~ 10 V, detection frequencies of 1 KHz ~ 1 MHz, DEP excitation signals with amplitude of 0 ~ 10 Vpp and frequencies of 1KHz ~ 1 MHz, which fully meets the demands of pDEP enrichment and impedance detection for bacteria. The microfluidic chip with interdigitated microelectrodes was manufactured by microfabrication methods. The interdigital microelectrode array has sufficient contact area with a bacterial suspension to improve enrichment efficiency and detection sensitivity. Bacteria in the interdigital microelectrode area of the microfluidic chip were firstly captured and enriched by pDEP. Then, in situ impedance detection of the enriched bacteria was realized by switching test conditions. Using the self-assembly microsystem, a novel quantitative detection method was established and demonstrated to detect Escherichia coli O157:H7. Experimental results showed that the detection limits of Escherichia coli O157:H7 was 5 × 10 cfu mL, and testing time was only 6 min under the optimized detection voltage of 100 mV and frequency of 500 KHz. The method was successfully used to detect Escherichia coli O157:H7 in synthetic chicken synthetic samples.

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Counting bacteria on a microfluidic chip

Cited by 44 publications

References 27 publications

Nanoparticle detection by microfluidic Resistive Pulse Sensor with a submicron sensing gate and dual detecting channels-two stage differential amplifier

Nanoparticle detection by microfluidic Resistive Pulse Sensor with a submicron sensing gate and dual detecting channels-two stage differential amplifier

A microfluidic device for label-free detection of Escherichia coli in drinking water using positive dielectrophoretic focusing, capturing, and impedance measurement

An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli

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