A microfluidic device for label-free detection of Escherichia coli in drinking water using positive dielectrophoretic focusing, capturing, and impedance measurement

Kim, Myounggon; Jung, Taekeon; Kim, Young‐Jin; Lee, Changgeun; Woo, K.B.; Seol, Jae Hun; Yang, Sung

doi:10.1016/j.bios.2015.07.059

Cited by 69 publications

(38 citation statements)

References 26 publications

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“…This can be evidenced in the growing number of published articles and citations reflected in Web of Science (WOS). This emergent trend is also evident for bacteria detection and concentration ( Figure 1 ) since several research groups reported the simultaneous measure of the concentrated bacteria in a single piece of equipment [ 14 , 30 , 35 , 36 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 73%

“…This generates an urgent necessity for fast, accurate, cost effective and more accessible technologies [ 25 ]. Due to this scenario, new methods of fast monitoring and characterization have been explored based on electrical properties of cells or particles [ 29 , 30 ]. In this context, electric field-based separation approaches are attracting interest because of their fastness, potential for automation, simplicity, portability, miniaturization, massive parallelization and labour-saving characteristics [ 10 , 11 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

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Combined Dielectrophoresis and Impedance Systems for Bacteria Analysis in Microfluidic On-Chip Platforms

Páez-Avilés

Juanola-Feliu

Punter-Villagrasa

et al. 2016

Sensors

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Bacteria concentration and detection is time-consuming in regular microbiology procedures aimed to facilitate the detection and analysis of these cells at very low concentrations. Traditional methods are effective but often require several days to complete. This scenario results in low bioanalytical and diagnostic methodologies with associated increased costs and complexity. In recent years, the exploitation of the intrinsic electrical properties of cells has emerged as an appealing alternative approach for concentrating and detecting bacteria. The combination of dielectrophoresis (DEP) and impedance analysis (IA) in microfluidic on-chip platforms could be key to develop rapid, accurate, portable, simple-to-use and cost-effective microfluidic devices with a promising impact in medicine, public health, agricultural, food control and environmental areas. The present document reviews recent DEP and IA combined approaches and the latest relevant improvements focusing on bacteria concentration and detection, including selectivity, sensitivity, detection time, and conductivity variation enhancements. Furthermore, this review analyses future trends and challenges which need to be addressed in order to successfully commercialize these platforms resulting in an adequate social return of public-funded investments.

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Section: Introductionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Combined Dielectrophoresis and Impedance Systems for Bacteria Analysis in Microfluidic On-Chip Platforms

Páez-Avilés

Juanola-Feliu

Punter-Villagrasa

et al. 2016

Sensors

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“…Therefore, integration of the developed biosensors with applicable pre-processing and post-processing techniques is of great importance for practical application on field. To date, microfluidics-based techniques have been widely integrated with biosensors for pre-concentration of bacteria cells, taking advantage of size difference [103,104], electrostatic trapping [105], gravity [106], dielectrophoresis [107][108][109] and ion-concentration polarization [110]. However, these techniques also have several limitations, for example, the limited volume of sample throughput, length of time required to complete the process and the requirement for relatively clean samples.…”

Section: Discussionmentioning

confidence: 99%

Phage-based Electrochemical Sensors: A Review

Chau

Lee

2019

Micromachines

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Phages based electrochemical sensors have received much attention due to their high specificity, sensitivity and simplicity. Phages or bacteriophages provide natural affinity to their host bacteria cells and can serve as the recognition element for electrochemical sensors. It can also act as a tool for bacteria infection and lysis followed by detection of the released cell contents, such as enzymes and ions. In addition, possible detection of the other desired targets, such as antibodies have been demonstrated with phage display techniques. In this paper, the recent development of phage-based electrochemical sensors has been reviewed in terms of the different immobilization protocols and electrochemical detection techniques.

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“…Over the last 2 decades, much effort has focused on resolving this issue by development of sample pretreatment methods that can be integrated with biosensors. These approaches include microfluidic separation (Zuo and others ; Packard and others ; Clime and others ; Kim and others ; Lee and others ; Luka and others ; Gashti and others ), nanoparticle separation (Varshney and Li ; Kwon and others ; Wang and others ), magnetic relaxation switching (Chen and others ), dielectrophoresis (Cheng and others ; Packard and others ; Wang and others ; Yang ; Hamada and others ; Couniot and others ; Kim and others ; Fernandez and others ), immunochromatography (Vyas and others ), acoustofluidic sorting (Li and others ), ferrofluidic manipulation (Kose and others ), or hydrodynamic focusing (Clime and others ). Although not reviewed in detail here, sample pretreatment is a major focus of biosensor research labs and the primary challenges are minimizing destructive sampling, need for pumps, energy, and use of exogenous chemicals.…”

Section: Introductionmentioning

confidence: 99%

Emerging Biorecognition and Transduction Schemes for Rapid Detection of Pathogenic Bacteria in Food

Vanegas

Gomes

Cavallaro

et al. 2017

Comp Rev Food Sci Food Safe

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The presence of unsafe levels of microorganisms in food constitutes a growing economic and public health problem that necessitates new technology for their rapid detection along the food continuum from production to consumption. While traditional techniques are reliable, there is a need for more sensitive, selective, rapid, and costeffective approaches for food safety evaluation. Methods such as microbiological counts are sufficiently accurate and inexpensive, and are capable of determining presence and viability for most pathogens. However, these techniques are time consuming, involve destructive sampling, and require trained personnel and biosafety-certified facilities for analysis. Molecular techniques such as the polymerase chain reaction have greatly improved analytical capability over the last decade, achieving shorter analysis time with quantitative data and strain specificity, and in some cases the ability to discriminate cell viability. The emerging field of nanosensors/biosensors has demonstrated a variety of devices that hold promise to bridge the gap between traditional plate counting and molecular techniques. Many nanosensors/biosensors are rapid, portable, accurate devices that can be used as an additional screening tool for identifying unsafe levels of microorganisms in food products with no need for pre-enrichment. In this review, we provide a brief overview of available biorecognitiontransduction techniques for detecting bacteria in food. We then discuss the advantages and disadvantages of each technique, and describe some recent biosensor or nanosensor technologies that are under development. We conclude by summarizing the opportunities and challenges in the field of pathogen monitoring in food systems and we focus the discussion on the strengths/weaknesses of the most popular biorecognition agents and transducer nanomaterials for biosensing.

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A microfluidic device for label-free detection of Escherichia coli in drinking water using positive dielectrophoretic focusing, capturing, and impedance measurement

Cited by 69 publications

References 26 publications

Combined Dielectrophoresis and Impedance Systems for Bacteria Analysis in Microfluidic On-Chip Platforms

Combined Dielectrophoresis and Impedance Systems for Bacteria Analysis in Microfluidic On-Chip Platforms

Phage-based Electrochemical Sensors: A Review

Emerging Biorecognition and Transduction Schemes for Rapid Detection of Pathogenic Bacteria in Food

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