Phage-based Electrochemical Sensors: A Review

Xu, Jia; Chau, Ying; Lee, Yi-Kuen

doi:10.3390/mi10120855

Cited by 44 publications

(34 citation statements)

References 107 publications

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“…Phages can bind to the gold surface trough van der Waals bonding, hydrophobic bonding and covalent bonding between the gold surface and the amine and thiol groups. This strategy is also used in surface plasmon resonance (SPR) sensor and quartz crystal microbalance (QCM) sensor using phage as a probe for pathogen detection [17]. Nevertheless, Lytic cycle: Phages attach to their host bacteria, insert their DNA and take over the host machinery to dissemination new virions that lyse the bacteria and infect a new host (lytic phages).…”

Section: Phages Wild Typementioning

confidence: 99%

“…Phages can bind to the gold surface trough van der Waals bonding, hydrophobic bonding and covalent bonding between the gold surface and the amine and thiol groups. This strategy is also used in surface plasmon resonance (SPR) sensor and quartz crystal microbalance (QCM) sensor using phage as a probe for pathogen detection [17]. Nevertheless, optimal condition of physical absorption has been found for lytic phages able to detect S. aureus by SPR with detection limit~10 4 cfu/mL −1 , or using magnetic elastic sensors [18] capable of detecting Salmonella (detection limit~10 3 cfu/mL −1 ) in milk and fat-free [19].…”

Section: Phages Wild Typementioning

confidence: 99%

“…Nevertheless, optimal condition of physical absorption has been found for lytic phages able to detect S. aureus by SPR with detection limit~10 4 cfu/mL −1 , or using magnetic elastic sensors [18] capable of detecting Salmonella (detection limit~10 3 cfu/mL −1 ) in milk and fat-free [19]. However, physical absorption has several limitations, such as non-specific and weak bonding between phage and sensor surface, leading to desorption during the analytic detection and low surface coverage of deposited phages [17].…”

Section: Phages Wild Typementioning

confidence: 99%

“…Xu et al designed micro-gold electrodes with phage T4 for the detection of E. coli. The sensitivity of this biosensor is in the range of 1.9 × 10 1 -1.9 × 10 8 CFU/mL bacteria [17]. Table 3 are summarized in several nanoparticles that have been used for detecting microorganisms.…”

Section: Amperometric Biosensorsmentioning

confidence: 99%

“…Bacteria can be detected by capturing electrons on the electrode surface. In EIS electrochemical sensors with bacteriophage, as a probe, are used to detect bacteria, trough catching the bacteria target the immobilized phages on electrodes, functional groups [17,80]. The main cause of this is the activity of lytic phages on bacteria, which causes the intracellular content to drop out and decrease the conductivity.…”

Section: Amperometric Biosensorsmentioning

confidence: 99%

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Bacteriophage Based Biosensors: Trends, Outcomes and Challenges

et al. 2020

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Foodborne pathogens are one of the main concerns in public health, which can have a serious impact on community health and health care systems. Contamination of foods by bacterial pathogens (such as Staphylococcus aureus, Streptococci, Legionella pneumophila, Escherichia coli, Campylobacter jejuni and Salmonella typhimurium) results in human infection. A typical example is the current issue with Coronavirus, which has the potential for foodborne transmission and ruling out such concerns is often difficult. Although, the possible dissemination of such viruses via the food chain has been raised. Standard bacterial detection methods require several hours or even days to obtain the results, and the delay may result in food poisoning to eventuate. Conventional biochemical and microbiological tests are expensive, complex, time-consuming and not always reliable. Therefore, there are urgent demands to develop simple, cheap, quick, sensitive, specific and reliable tests for the detection of these pathogens in foods. Recent advances in smart materials, nanomaterials and biomolecular modeling have been a quantum leap in the development of biosensors in overcoming the limitations of a conventional standard laboratory assay. This research aimed to critically review bacteriophage-based biosensors, used for the detection of foodborne pathogens, as well as their trends, outcomes and challenges are discussed. The future perspective in the use of simple and cheap biosensors is in the development of lab-on-chips, and its availability in every household to test the quality of their food.

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Section: Phages Wild Typementioning

confidence: 99%

“…Phages can bind to the gold surface trough van der Waals bonding, hydrophobic bonding and covalent bonding between the gold surface and the amine and thiol groups. This strategy is also used in surface plasmon resonance (SPR) sensor and quartz crystal microbalance (QCM) sensor using phage as a probe for pathogen detection [17]. Nevertheless, optimal condition of physical absorption has been found for lytic phages able to detect S. aureus by SPR with detection limit~10 4 cfu/mL −1 , or using magnetic elastic sensors [18] capable of detecting Salmonella (detection limit~10 3 cfu/mL −1 ) in milk and fat-free [19].…”

Section: Phages Wild Typementioning

confidence: 99%

Section: Phages Wild Typementioning

confidence: 99%

Section: Amperometric Biosensorsmentioning

confidence: 99%

Section: Amperometric Biosensorsmentioning

confidence: 99%

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Bacteriophage Based Biosensors: Trends, Outcomes and Challenges

et al. 2020

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Disposable electrochemical biosensors for the detection of bacteria in the light of antimicrobial resistance

C. S.,

Kini,

Singh

et al. 2024

Biotech & Bioengineering

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Persistent and inappropriate use of antibiotics is causing rife antimicrobial resistance (AMR) worldwide. Common bacterial infections are thus becoming increasingly difficult to treat without the use of last resort antibiotics. This has necessitated a situation where it is imperative to confirm the infection to be bacterial, before treating it with antimicrobial speculatively. Conventional methods of bacteria detection are either culture based which take anywhere between 24 and 96 hor require sophisticated molecular analysis equipment with libraries and trained operators. These are difficult propositions for resource limited community healthcare setups of developing or less developed countries. Customized, inexpensive, point‐of‐care (PoC) biosensors are thus being researched and developed for rapid detection of bacterial pathogens. The development and optimization of disposable sensor substrates is the first and crucial step in development of such PoC systems. The substrates should facilitate easy charge transfer, a high surface to volume ratio, be tailorable by the various bio‐conjugation chemistries, preserve the integrity of the biorecognition element, yet be inexpensive. Such sensor substrates thus need to be thoroughly investigated. Further, if such systems were made disposable, they would attain immunity to biofouling. This article discusses a few potential disposable electrochemical sensor substrates deployed for detection of bacteria for environmental and healthcare applications. The technologies have significant potential in helping reduce bacterial infections and checking AMR. This could help save lives of people succumbing to bacterial infections, as well as improve the overall quality of lives of people in low‐ and middle‐income countries.

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Recent Advances on Electrochemical Sensors Based on Electroactive Bacterial Systems for Toxicant Monitoring: A Minireview

Haddour

Azri

2022

Electroanalysis

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In recent years, there have been advancements in the development of bacterial electrochemical sensors for toxicity monitoring, especially through utilization of electroactive bacteria. Accordingly, this mini review summarizes the recent advances in the design of bacterialbased electrochemical sensors with a specific discussion of main methodologies used for preparation of bioelectrodes based on electroactive bacteria. Additionally, current trends in the design of efficient and high performing bacterial electrochemical sensors for toxicity monitoring are presented. An overview of the most relevant findings and challenges of this technology for practical are provided and might serve as a general outlook for planning further research.

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Phage-based Electrochemical Sensors: A Review

Cited by 44 publications

References 107 publications

Bacteriophage Based Biosensors: Trends, Outcomes and Challenges

Bacteriophage Based Biosensors: Trends, Outcomes and Challenges

Disposable electrochemical biosensors for the detection of bacteria in the light of antimicrobial resistance

Recent Advances on Electrochemical Sensors Based on Electroactive Bacterial Systems for Toxicant Monitoring: A Minireview

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