Counterflow immunoisotachophoresis on cellulose acetate membranes

Абелев, Г. И.; Karamova, E.R.

doi:10.1016/0003-2697(84)90487-1

Cited by 25 publications

(21 citation statements)

References 8 publications

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“…Our data correspond very well to the concept that suggests that immunodominant epitopes of different proteins are created by 'mobile' regions of their molecules [20]. The possible equilibrium between 'open' and 'cryptic' forms for several epitopes is in line with such a concept.…”

Section: Discussionsupporting

confidence: 74%

“…As the cellulose acetate membrane does not absorb protein, the AFP was concentrated before the dye front ( fig. 3c), as shown previously [20]. After the AFP had migrated from the nitrocellulose onto Cellogel, the region before the dye front containing the AFP was cut out, eluted with 0.06 M Tris-HCl buffer (pH 6.8) and subjected to PAGE and immunoblotting under nondenaturating conditions.…”

Section: Iae 'Filtration'mentioning

confidence: 99%

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New Approaches for the Detection and Characterization of Alpha-Fetoprotein Epitope Variants

Karamova¹,

Yazova²,

Yakimenko³

et al. 2003

Tumor Biol

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Alpha-fetoprotein (AFP) is an embryo-specific protein of mammalian sera that consists of a single glycosylated polypeptide chain (∼70 kD) with 5–6 epitope clusters located on the native molecule. We have elaborated three approaches for the separation of AFP into fractions (variants) that are different in the distinct epitope expression on the native molecule. For this, we have used three technologies largely based on immunoaffinity electrochromatography and electrophoresis/immunoblotting. The separation of these variants was facilitated using monoclonal antibodies previously characterized to the 5 epitope clusters and two individual epitopes on AFP in the ISOBM international workshop. We have shown that these approaches facilitate the identification of cryptic epitopes in AFP, and the relevance of these findings is discussed.

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Section: Discussionsupporting

confidence: 74%

Section: Iae 'Filtration'mentioning

confidence: 99%

New Approaches for the Detection and Characterization of Alpha-Fetoprotein Epitope Variants

Karamova¹,

Yazova²,

Yakimenko³

et al. 2003

Tumor Biol

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“…At a certain stage, oppositely directed flows equalize each other; then, the moving boundary of the leading and terminating anions and, respectively, the zone of separation of proteins stop. The theory of successive stages in counterflow isotachophoresis and practical details of this method were described in [3][4][5][6][7].…”

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confidence: 99%

Counterflow Isotachophoresis As a Method of Concentration and Isolation of DNA from Biological Fluids

Кондратова¹,

Serdyuk²,

Шелепов³

et al. 2005

Dokl Biochem Biophys

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“…We have modified this method using cellulose-acetate membranes (CAM) as a supporting medium for ITP (ITP-CAM) (Abelev and Karamova, 1984). This made it possible to use electro-osmotic counterflow in CAM to sharply increase the concentration of a given substance, and to simultaneously detect the antigens separated on CAM.…”

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confidence: 99%

“…High titers of K or A chains suggested the presence of BJP. The light-chain titers were taken into account when the dose of urine sample for ITP-CAM was detected.(b) ITP-CAM was carried out as described by Abelev and Karamova (1984). Briefly, CAM strips (Cellogel, Chemetron, Milan, Italy) 1.5 cm X 17 cm with 2 folds were used for analysis.…”

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confidence: 99%

Immuno‐isotachophoretic determination of monoclonal immunoglobulin light chains produced by neoplastic b‐cells: Use in diagnosis, monitoring and detection of residual disease

Абелев

Karamova

Bragina³

et al. 1990

Intl Journal of Cancer

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We suggest that countercurrent isotachophoresis performed on cellulose acetate membranes (ITP-CAM) should be used for detecting trace amounts of Bence-Jones protein (BJP) in urine of patients with chronic lymphocytic leukemia (CLL), non-Hodgkin's lymphoma (NHL) and related diseases. ITP-CAM allows simultaneous concentration and electrophoretic separation of proteins present in highly diluted solutions, as well as easy immunological detection of separated substances. BJP was found in 24 out of 42 patients with CLL, 33 of 56 with NHL and 3 of 3 with Waldenström macroglobulinemia. Twenty-three patients were followed during the course of chemo- or radiotherapy. In 19 cases the BJP findings correlated well with clinical status. In no case of partial or complete clinical response did BJP completely disappear from the urine.

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Counterflow immunoisotachophoresis on cellulose acetate membranes

Cited by 25 publications

References 8 publications

New Approaches for the Detection and Characterization of Alpha-Fetoprotein Epitope Variants

New Approaches for the Detection and Characterization of Alpha-Fetoprotein Epitope Variants

Counterflow Isotachophoresis As a Method of Concentration and Isolation of DNA from Biological Fluids

Immuno‐isotachophoretic determination of monoclonal immunoglobulin light chains produced by neoplastic b‐cells: Use in diagnosis, monitoring and detection of residual disease

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