Countercurrent Chromatography

A Simple Isolation Method for the Major Catechins in Green Tea Using High-Speed Countercurrent Chromatography

“…Subsequently, the catechin portion was submitted to high-speed centrifugal countercurrent chromatography (HSCCC). A broad range of HSCCC-compatible solvent systems was evaluated, in particular n -hexanes−ethyl acetate− n -butanol−methanol−water, n -hexanes−ethyl acetate−water, ethyl acetate−methanol−water, and chloroform−methanol− n -butanol−water systems of various proportions. − Partitioning behavior of the catechins was assessed by TLC analysis of the two phases. The solvent systems n -hexanes−ethyl acetate−water (e.g., 1:5:5) and ethyl acetate−methanol−water (5:1:5 and 5:2:5) showed favorable partitioning between the two layers.…”