A Simple Isolation Method for the Major Catechins in Green Tea Using High-Speed Countercurrent Chromatography

Abstract

An efficient protocol for the preparative purification of the major tea catechins (-)-epicatechin gallate (3) and epigallocatechin gallate (5) has been developed, employing liquid-liquid partitioning, high-speed countercurrent chromatography, and gel chromatography for final purification. The method is suitable for scale-up to significantly larger quantities.

Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis

“…A simple and efficient method for separation of catechin gallates from spray-dried tea extract was developed by Baumann et al [376]. Tea phenolic extract was first subjected to liquid-liquid partitioning between ethyl acetate and water.…”

Extraction, separation, and detection methods for phenolic acids and flavonoids

“…Baumann et al developed a simple and efficient procedure for the separation of catechin gallates from spraydried tea extract [82]. Tea phenolic extract was first subjected to liquid -liquid partitioning between ethyl acetate and water.…”

A MEKC method for naringenin from natural and biological samples

“…They include HPLC-UV, [11][12][13] HPLC with photodiodearray, 14 gas chromatography, 15 gas chromatography with mass spectrometry 16 and liquid chromatography with mass spectrometry. 19 Most studies that use CE for the analysis of avonoids are in the eld of natural product research, including the analysis of plants, [20][21][22] vegetables, 23 herbs 24 and other plant or fruit-derived products. 17 During the past decade, capillary electrophoresis (CE) has become one of the versatile approaches for highly efficient separation.…”