Counter immunoelectrophoresis as a rapid screening test for amoebic liver abscess.

Tosswill, Jennifer; Ridley, D. S.; Warhurst, D. C.

doi:10.1136/jcp.33.1.33

Cited by 9 publications

(3 citation statements)

References 4 publications

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“…For this study we defined patients as harboring an amebic liver abscess in cases with a clinically suspect history and with radiological proof of one or more space-occupying lesions in the liver, in combination with a positive cellulose acetate precipitation test result on the day of presentation. The CAP test is a highly sensitive and specific test for serodiagnosis of amebic liver abscess which becomes negative relatively quickly after successful treatment (29). For all 27 patients with amebic liver abscess in this study, both the ELISA and Dipstick were positive in serum samples taken at the first time of clinical suspicion.…”

Section: Discussionmentioning

confidence: 78%

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Use of Rapid Dipstick and Latex Agglutination Tests and Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Amebic Liver Abscess, Amebic Colitis, and Entamoeba histolytica Cyst Passage

Doorn¹,

Hofwegen²,

Koelewijn³

et al. 2005

J Clin Microbiol

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A homemade enzyme-linked immunosorbent assay (ELISA) and a dipstick assay (Dipstick) for the detection of anti-Entamoeba histolytica antibodies in serum were developed and evaluated together with a commercially available latex agglutination test (LAT; Laboratoires Fumouze) for their use in serodiagnosis of amebiasis. The sensitivity of these assays was evaluated with sera from 27 patients with radiologically proven, cellulose acetate precipitation (CAP) test-positive amebic liver abscess, 7 patients with parasitologically and PCR-proven amebic colitis, and 11 patients with parasitologically and PCR-proven E. histolytica cyst passage. The sensitivities of the ELISA, Dipstick, and LAT were all 93.3% (42/45). With a combination of Dipstick and LAT, all abscess and colitis patients had at least one positive result. The specificity was assessed with 238 sera from patients with various parasitic, bacterial, viral, and fungal infectious diseases, sera containing autoimmune antibodies, and sera from healthy blood donors. The specificities of the ELISA, Dipstick, and LAT were 97.1%, 98.1%, and 99.5%, respectively. Of 61 sera from patients with PCR-proven E. dispar infection, 60 (98.4%) were negative in both Dipstick and LAT and 59 (96.7%) were negative in ELISA. Our data suggest that all three assays are sensitive serological tests. The rapid LAT and Dipstick provide fast results and can easily be applied in routine laboratories in order to facilitate the diagnosis of amebiasis.

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Section: Discussionmentioning

confidence: 78%

“…This test was described before and is regarded a reference test for amebic serology (26,29). The test is based on the principle of counterimmunoelectrophoresis.…”

Section: Methodsmentioning

confidence: 99%

Use of Rapid Dipstick and Latex Agglutination Tests and Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Amebic Liver Abscess, Amebic Colitis, and Entamoeba histolytica Cyst Passage

Doorn¹,

Hofwegen²,

Koelewijn³

et al. 2005

J Clin Microbiol

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show abstract

“…In some other parasitic disease it was seen that purification of antigens remains an obstacle for serodiagnos is in all these conditions but is rapidly progressing; for instance, the purification of schistosoma-egg antigen should further increase the test's specificity and sensitivity in this disease [11,14]. Evaluation of the ELISA enzyme-linked procedure is not yet complete in some other protozoal infections, but current methods such as counter electrophoresis in occult amoebic abscess or immunofluorescent methods in giardiasis yield acceptable results [15,16].…”

Section: Toxoplasmosismentioning

confidence: 99%