Coumarins as Antimitotics

Podbielkowska, Maria; Kupidłowska, Ewa; Wałęza, Maria; Dobrzyńska, Katarzyna; Louis, Stodieck; Keightley, Andrew; Zobel, Alicja M.

doi:10.3109/13880209409083004

Cited by 19 publications

(18 citation statements)

References 34 publications

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“…In this study, a modification of Podbielkowska's assay (12) was introduced as a simple method to evaluate the effect of drugs on the mitotic process. In order to eliminate any significant differences during sampling, a zero time sampling for all bulbs was examined under the same conditions.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of natural anthracene-derived compounds as antimitotic agents

Badria

Ibrahim

2013

Drug Discov Ther

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Plants that contain anthracenederived compounds such as anthraquinones have been reported to act as anticancer besides their use for millennia to treat constipation, but the mechanism of action is still unfolding. Therefore we pursue this study to explore a new horizon in the anticancer property of these agents with relevance to mitotic arrest. To achieve this goal, the antimitotic activity of a series of naturally occurring anthracene-derived anthraquinones including anthrone, alizarin (1,2-dihydroxyanthraquinone), quinizarin (1,4-dihydroxyanthraquinone), rhein (4,5-dihydroxyanthraquinone-2-carboxylic acid), emodin (1,6,8-trihydroxy-3-methyl-anthraquinone), and aloe emodin (1,8 dihydroxy-3-hydroxymethyl anthraquinone) were evaluated using Allium cepa root tips. Initial results revealed that the mitosis was inhibited after 3, 6, and 24 h, respectively, of incubation with 500, 250, and 125 ppm of each compound in a dose-dependent manner. Furthermore, alizarin at 500 ppm was proved to be the most active compound to arrest the mitosis after 24 h followed by emodin, aloe emodin, rhein, and finally quinizarin. Interestingly, this inhibition of mitosis was irreversible in root tips incubated with each compound at concentration of 500 ppm but not with 250 ppm or 125 ppm, where the roots regained their normal mitotic activity after 96 h post-incubation in water. This re-evaluation of an old remedy suggests that several bioactive anthraquinones possess promising anti-mitotic activity that may have the potential to be lead compounds for the development of a new class of multifaceted natural anticancer/antimitotic agents.

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Section: Methodsmentioning

confidence: 99%

Evaluation of natural anthracene-derived compounds as antimitotic agents

Badria

Ibrahim

2013

Drug Discov Ther

View full text Add to dashboard Cite

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“…The frequency of each of the mitotic phases was analyzed as the percentage of cells in a particular phase in 200 dividing cells according to Lopez-Saez and Fernandez-Gomez (1965). The ratios of metaphase to anaphase plus telophase (M/A+T), and metaphase to prophase (M/P), were calculated to ascertain whether any blockage of a particular phase had occurred (Podbielkowska et al, 1994). Light microscopy was employed to assess morphological changes to the cell population of promeristematic cells.…”

Section: Methodsmentioning

confidence: 99%

“…Coumarin (1,2-benzopyrone) is a higher plantderived secondary metabolite capable of altering the energy metabolism of cells by inhibiting glycolysis and oxidative phosphorylation (Kupidlowska et al, 1994(Kupidlowska et al, , 1996. Coumarin has been found to be a promising anticancer drug as it has exhibited antimitotic action on promeristematic cells of Allium cepa L. in Levan's test (Podbielkowska et al, 1994;Keightley et al, 1996) and lowered the vitality of melanoma cells without causing chromosomal aberrations (Wierzchowska-Renke et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Coumarin has been found to be a promising anticancer drug as it has exhibited antimitotic action on promeristematic cells of Allium cepa L. in Levan's test (Podbielkowska et al, 1994;Keightley et al, 1996) and lowered the vitality of melanoma cells without causing chromosomal aberrations (Wierzchowska-Renke et al, 1998). …”

Section: Introductionmentioning

confidence: 99%

“…Levan's test (Levan, 1938;Lopez-Saez & Fernandez-Gomez, 1965) has been used to examine the Keywords: Allium, paclitaxel, coumarin, metabolite, microtubule, antimitotic, clastogenic, cytotoxic, cancer. mitodepressive and clastogenic activity induced by various chemicals (Podbielkowska & Waleza, 1980;Podbielkowska, 1981Podbielkowska, , 1994 on root promeristem belonging to the Allium species of plants. We have used Levan's test to investigate the antimitotic and clastogenic effects induced by paclitaxel alone and when combined with coumarin at varying concentrations on meristematic cell populations of Allium sativum L. The combination of paclitaxel and coumarin together with their individual reaction mechanisms will assist in evaluating how these two natural product agents interact synergistically at the biochemical level.…”

Section: Introductionmentioning

confidence: 99%

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