Coumarin and its 4- and 7- Substituted Derivatives as Retardants of Mitoses in Allium Root Promeristem

Effect of Alkaloid-Free and Alkaloid-Rich preparations from Uncaria tomentosa bark on mitotic activity and chromosome morphology evaluated by Allium Test

“…Dawidowska were used for the investigation as previously described (Kuraś and Malinowska, 1978;Keightley et al, 1996;Majewska et al, 2000;Kuraś et al, 2006). The roots were grown in distilled water in 200 mL Erlenmeyer flasks under laboratory conditions (on light, 24 • C).…”

“…Chromosome morphology and their changes were photographed under a light microscope (NU Zeiss), using a photographic camera (Nikon). A parallel control treatment in distilled water was employed as previously described (Podbielkowska et al, 1994(Podbielkowska et al, , 1995Keightley et al, 1996).…”

Changes in chromosome structure, mitotic activity and nuclear DNA content from cells of Allium Test induced by bark water extract of Uncaria tomentosa (Willd.) DC

“…In order to study the mechanism and broad range of action of the water extracts of Uncaria tomentosa bark, the objective of this work was to determine the influence of the extract on meristematic cells of root tips of Allium cepa L. This model root system of plant cells is commonly used as a test for investigating environmental pollution factors, toxicity of chemical compounds and evaluating potential anticancer properties (Keightley et al, 1996;Kupidłowska et al, 1994;Kuraś and Malinowska, 1978;Majewska et al, 2003;Podbielkowska et al, 1981Podbielkowska et al, , 1995. It has been used since 1938 (Levan, 1938).…”

Apiaceae Lindl.

“…Some species, such as Ammi majus, are used as a source of xanthotoxin for photochemotherapy of psoriasis (de Wolff and Thomas 1986). Taxa containing furanocoumarins such as psoralen and xanthotoxin are also known to inhibit mitosis by disrupting spindle function and have been employed as abortifacients in some cultures (Podbielkowska et al 1995;Keightley et al 1996;Madari and Jacobs 2004). Under these conditions psoralen -DNA crosslinkages increase the risk of development of squamous cell carcinomas and possibly melanomas with long -term high dosage (Stern et al 1997;Stern and Lunder 1998;Stern 2001).…”