Effect of Alkaloid-Free and Alkaloid-Rich preparations from Uncaria tomentosa bark on mitotic activity and chromosome morphology evaluated by Allium Test

Kuraś, Mieczysław; Pilarski, Radosław; Nowakowska, Julita; Zobel, Alicja M.; Brzost, Krzysztof; Antosiewicz, J.; Gulewicz, Krzysztof

doi:10.1016/j.jep.2008.10.023

Cited by 21 publications

(9 citation statements)

References 34 publications

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“…Such deformation of genetic material together with sticking of chromosomes, inhibits the normal progress of mitotic division even long after removal of the causative factor. [29]…”

Section: Discussionmentioning

confidence: 99%

Radioprotective and cytoprotective activity of Tinospora cordifolia stem enriched extract containing cordifolioside-A

Patel¹,

Bigoniya²,

Singh³

et al. 2013

Indian J Pharmacol

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Objectives:The present study was undertaken to evaluate the radioprotective and cytoprotective potential of cordifolioside-A, a primary active constituent of n-butanol fraction of Tinospora Cordifolia (NBTC) against 4 Gy-γ radiation in mice and cyclophosphamide induced genotoxicity.Materials and Methods:Presence of cordifolioside-A in NBTC stem ethanolic extract was confirmed by high performance thin layer chromatography (HPTLC) analysis. Radioprotective activity was evaluated at 80 and 120 mg/kg, intraperitoneal (i.p.) dose of NBTC administered 15 days prior to whole body radiation exposure by observing survival rate, change in body weight, hematology, spleen colony forming unit (CFU), and micronucleus (MN) expression. Cytoprotective activity of NBTC was evaluated at 5, 10, and 15 mg/ml concentrations on Allium cepa root meristem growth against cyclophosphamide.Results:HPTLC analysis of standard cordifolioside A, and NBTC confirmed the presence of cordifolioside-A in NBTC with the retention factor value of 0.86. Administration of NBTC (120 mg/kg, i.p.) produced significant protection against radiation in terms of increased survival rate, body weight retention, hematological parameters, spleen CFU assay (P < 0.01), and decreased MN expression (P < 0.01). Cytoprotectivity was observed maximally at 10 mg/ml NBTC concentration with significant increase in root growth (P < 0.01), non-toxic mitotic index (MI) (65.9%) and lesser chromosomal aberrations (15.4%). NBTC at 10 mg/ml concentration showed very few C-anaphase compared to aberrations like fragmentation, C-anaphase, multipolarity and sticky chromosome in cyclophosphamide alone.Conclusion:The results suggest that enriched NBTC containing cordifolioside-A has a potential in vivo radioprotective effect as well as in vitro cytoprotective activity.

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“…Such deformation of genetic material together with sticking of chromosomes, inhibits the normal progress of mitotic division even long after removal of the causative factor. [29]…”

Section: Discussionmentioning

confidence: 99%

Radioprotective and cytoprotective activity of Tinospora cordifolia stem enriched extract containing cordifolioside-A

Patel¹,

Bigoniya²,

Singh³

et al. 2013

Indian J Pharmacol

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“…Flavonoids in fruits are important sources of natural antioxidants that are preferred over synthetic ones as they are less toxic (Ifesan et al, 2013). Flavonoids scavenge free radicals produced by Reactive Oxygen Species (ROS) thereby preventing diseases caused by oxidative stress (Kuraś et al, 2009). DPPH (2,2-Diphenyl-1-picrylhydrazyl) is widely used to test the ability of compounds to act as free radical scavengers or hydrogen donors, and to evaluate antioxidant activity of foods.…”

Section: Antioxidant Propertiesmentioning

confidence: 99%

Influence of freezing and oven air drying on the total monomeric anthocyanins, vitamin c and antioxidant capacity of African star apple

Olusegun¹

2019

Res. J. Food Sci. Nutr.

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African star apple is a good source of vitamin C and antioxidants. The choice of method for preserving its antioxidant properties is a major decision. The aim of this study was to determine the proximate composition, physical properties, total monomeric anthocyanins contents, vitamin C and antioxidant capacity of different parts (the pulp, the seed, and the peel) of the African star apple fruit depending on the method of preservation in the process of freezing, and oven air drying. During oven air drying and freeze drying, losses of vitamin C as compared with fresh fruit parts resulted averagely in 67% and 13% respectively for the seeds, 19% and 4% for the pulp. Freeze drying process was characterized as the best preservation method to contain relative amount of anthocyanins, vitamin C and antioxidants.

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“…Numerous comparative studies have been conducted using plant, animal and human tests to evaluate the activity of different compounds, and their results are largely consistent (Nilan 1978;Grant 1994;Kuraś et al 2006,;Abratowska et al 2014;Wierzbicka et al 2015). The Allium test (Levan 1938) is based on the observation of chromosome divisions in root meristematic cells of Allium cepa L. This test is used to investigate the effects of various factors on mitotic activity, life cycle, aberration induction, structure and ultrastructure of cells, cellular respiration and many other living processes (Kuraś et al 2006(Kuraś et al , 2009 including, but not limited to, ( Kuraś et al 2006). Kuraś et al (2009) in alkaloid-rich and alkaloid-free fractions (different in chemical composition) obtained from U. tomentosa bark observed condensation and contraction of chromosomes (more in alkaloid-rich) with retardation and/or inhibition of mitoses and changed mitotic phases.…”

Section: Phytotoxkit Biotestmentioning

confidence: 85%

“…The Allium test (Levan 1938) is based on the observation of chromosome divisions in root meristematic cells of Allium cepa L. This test is used to investigate the effects of various factors on mitotic activity, life cycle, aberration induction, structure and ultrastructure of cells, cellular respiration and many other living processes (Kuraś et al 2006(Kuraś et al , 2009 including, but not limited to, ( Kuraś et al 2006). Kuraś et al (2009) in alkaloid-rich and alkaloid-free fractions (different in chemical composition) obtained from U. tomentosa bark observed condensation and contraction of chromosomes (more in alkaloid-rich) with retardation and/or inhibition of mitoses and changed mitotic phases. The results described in these works (Kuraś et al 2006(Kuraś et al , 2009 overlap with those obtained in our work for water extracts of Vilcacora bark.…”

Section: Phytotoxkit Biotestmentioning

confidence: 99%

“…Moreover, Vilcacora contains a range of other compounds, such as polyphenols, sterols, quinic acid glycosides, triterpenes and their derivatives, that work synergistically with alkaloids. These compounds have anti-inflammatory and antiviral properties, protect blood vessels and remove reactive oxygen species which are known to induce DNA damage in cells (Sheng et al 1998;Wurm et al 1998;Keplinger et al 1999;Falkiewicz and Łukasiak 2001;Sandoval et al 2002;Kuraś et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Mitotic activity of a new phytotherapeutic product with a trade name of “Ganomix”

et al. 2016

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Water extracts of Uncaria tomentosa (Vilcacora) bark and Ganoderma lucidum (Reishi) spores that are rich in biologically active compounds, are increasingly widely used in prevention and treatment of many serious medical conditions. They are considered an important treatment option, complementary to phytotherapeutics and synthetic medicines. A new formulation with a trade name of Ganomix was developed based on these two components. The aim of this study was to determine mitotic activity of Ganomix and to compare the activity of Ganomix with the activity of its individual components. The biological activity was assessed using the following plant tests: Allium test (Levan test) and Phytotoxkit biotest. In addition, the chemical composition of selected components was analyzed. Water extracts of Vilcacora bark and Reishi spores, as well as their combination in Ganomix, exhibited varying biological activities. The Vilcacora bark extracts had inhibitory effects at the great majority of concentrations tested, while Reishi spore extracts were mainly stimulatory at the same range of concentrations. However, Ganomix extract showed an intermediate effect compared with Vilcacora bark and Reishi spore extracts. The promising results of these investigations give hope for the effective use of Ganomix in prevention and therapy of different sicknesses.

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Effect of Alkaloid-Free and Alkaloid-Rich preparations from Uncaria tomentosa bark on mitotic activity and chromosome morphology evaluated by Allium Test

Cited by 21 publications

References 34 publications

Radioprotective and cytoprotective activity of Tinospora cordifolia stem enriched extract containing cordifolioside-A

Radioprotective and cytoprotective activity of Tinospora cordifolia stem enriched extract containing cordifolioside-A

Influence of freezing and oven air drying on the total monomeric anthocyanins, vitamin c and antioxidant capacity of African star apple

Mitotic activity of a new phytotherapeutic product with a trade name of “Ganomix”

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