Could Cisplatin Loading on Biosynthesized Silver Nanoparticles Improve Its Therapeutic Efficacy on Human Prostate Cancer Cell Line and Reduce Its In Vivo Nephrotoxic Effects?

El-Sheikh, Sawsan M.A.; Edrees, Nagah; El-sayed, Hend; Khamis, Tarek; Arisha, Ahmed Hamed; Metwally, Mohamed M.M.; Eleiwa, Naglaa Z.H.; Galal, Azza A.A.

doi:10.1007/s12011-021-02677-3

Cited by 9 publications

(5 citation statements)

References 37 publications

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“…To date, there are several studies reporting the effect of AgNPs synthesized by different methods and with a variety of cappings and their cytotoxic effect in PCa cell lines. [48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63] Among these studies, the mechanism of action is, most of the times, common to the one we found in the present study, with an increase in ROS production, mitochondrial damage, nuclear fragmentation and cell apoptosis. 50,52,60,62,63 However, most of them do not compare AgNPs' effect in hormonesensitive and hormone-resistant cell lines and, to the best of our knowledge, the present study is the first one reporting the effect of AgNPs with a glucose functionalization in PCa and with a selective effect towards hormone resistance in this tumor model.…”

supporting

confidence: 72%

Glucose-Functionalized Silver Nanoparticles as a Potential New Therapy Agent Targeting Hormone-Resistant Prostate Cancer cells

Morais¹,

Machado²,

Dias³

et al. 2022

IJN

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Purpose Silver nanoparticles (AgNPs) have shown great potential as anticancer agents, namely in therapies’ resistant forms of cancer. The progression of prostate cancer (PCa) to resistant forms of the disease (castration-resistant PCa, CRPC) is associated with poor prognosis and life quality, with current limited therapeutic options. CRPC is characterized by a high glucose consumption, which poses as an opportunity to direct AgNPs to these cancer cells. Thus, this study explores the effect of glucose functionalization of AgNPs in PCa and CRPC cell lines (LNCaP, Du-145 and PC-3). Methods AgNPs were synthesized, further functionalized, and their physical and chemical composition was characterized both in water and in culture medium, through UV-visible spectrum, dynamic light scattering (DLS), transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR). Their effect was assessed in the cell lines regarding AgNPs’ entering pathway, cellular proliferation capacity, ROS production, mitochondrial membrane depolarization, cell cycle analysis and apoptosis evaluation. Results AgNPs displayed an average size of 61nm and moderate monodispersity with a slight increase after functionalization, and a round shape. These characteristics remained stable when redispersed in culture medium. Both AgNPs and G-AgNPs were cytotoxic only to CRPC cells and not to hormone-sensitive ones and their effect was higher after functionalization showing the potential of glucose to favor AgNPs’ uptake by cancer cells. Entering through endocytosis and being encapsulated in lysosomes, the NPs increased the ROS, inducing mitochondrial damage, and arresting cell cycle in S Phase, therefore blocking proliferation, and inducing apoptosis. Conclusion The nanoparticles synthesized in the present study revealed good characteristics and stability for administration to cancer cells. Their uptake through endocytosis leads to promising cytotoxic effects towards CRPC cells, revealing the potential of G-AgNPs as a future therapeutic approach to improve the management of patients with PCa resistant to hormone therapy or metastatic disease.

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supporting

confidence: 72%

Glucose-Functionalized Silver Nanoparticles as a Potential New Therapy Agent Targeting Hormone-Resistant Prostate Cancer cells

Morais¹,

Machado²,

Dias³

et al. 2022

IJN

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“…In fact, the AgNPs inhibited the release of AIF and cytochrome c from mitochondria via reducing the activation and translocation of Bax into mitochondrial membranes, which in turn prevents caspase-dependent apoptosis in kidneys. Interestingly, the renal tissue of mice treated with AgNPs was almost normal [ 54 ].…”

Section: Metallic Nano-formulationsmentioning

confidence: 99%

“…Moreover, CDDP-induced glomerular damage includes congestion, Bowman's capsule collapse, atrophy, necrosis, thickening basement membrane, widening Bowman's space, and capsule deformation. As summarized in Table 3 , we can see that the reviewed nanoparticles have been able to neutralize the histopathological effects of CDDP on renal tissues [ 54 , 57 , 58 , 72 , 87 , 99 , 120 ].…”

Section: Polymeric Nano-formulationsmentioning

confidence: 99%

“… -Anti-oxidant and anti-inflammatory activities -Decreased: urea, creatinine, lipid peroxidation, pro-inflammatory cytokines, and histopathological damages -Increased: catalase and GSH [ 53 ] Green silver nanoparticles (CP-AgNPs) 33.2 – Wistar rats (2.5 mg/kg) (i.p.) -Inhibiting mitochondria-mediated apoptosis - Decreased: Bax, caspase-3, and histopathological damages -Increased: Bcl-2 -Inhibited: the releases of AIF and cytochrome c from mitochondria [ 54 ] N-(2-hydroxyphenyl) acetamide-conjugated gold nanoparticles (NA2-AuNPs) 25–60 −45.7 Balb/c mice (Different doses) (i.p.) -As a nano-carrier for a natural anti-oxidant -Downregulated: NF-κB, iNOS and IL-6 -Upregulated: HO-1 [ 55 ] Ficus carica L. leaves extract-loaded AuNPs 100 – Albino rats (0.5 ml of Au NPs/Fig mixture as different v/v ratios) (orally) -As a nano-carrier for a natural anti-oxidant -Scavenging ROS -Mitigating AKI severity [ 56 ] Selenium nanoparticles (SeNPs) 45.9 – Pretreatment of albino rats with both SeNPs (0.5 mg/kg; orally) and fish oil before cisplatin administration and γ-radiation -Inhibiting caspase-dependent apoptosis and inflammation -Strengthening the antioxidant system -Decreased: urea, creatinine, TNF-α, caspase-3, cyclooxygenase-2, and histopathological damages [ 57 ] Tea polyphenol (TP)-functionalized SeNPs (Se@TE) Under microwave conditions: -After 0.5 h: 200.8 -After 1 h: 216.4 -After 2 h: 220.4 -After 0.5 h: − 9.4 -After 1 h: − 11.5 -After 2 h: − 10.7 -In vitro: HK-2 cells -In vivo: KM mice (1 and 2 mg/kg) (i.v.)…”

Section: Introductionmentioning

confidence: 99%

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Ameliorative impacts of polymeric and metallic nanoparticles on cisplatin-induced nephrotoxicity: a 2011–2022 review

et al. 2022

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Cisplatin (CDDP) is a well-known platinum-based drug used in the treatment of various malignancies. However, the widespread side effects that this drug leaves on normal tissues make its use limited. Since cisplatin is mainly eliminated from the kidneys, CDDP-induced nephrotoxicity is the most significant dose-limiting complication attributed to cisplatin, which often leads to dose withdrawal. Considering the high efficiency of cisplatin in chemotherapy, finding renoprotective drug delivery systems for this drug is a necessity. In this regard, we can take advantages of different nanoparticle-based approaches to deliver cisplatin into tumors either using passive targeting or using specific receptors. In an effort to find more effective cisplatin-based nano-drugs with less nephrotoxic effect, the current 2011–2022 review study was conducted to investigate some of the nanotechnology-based methods that have successfully been able to mitigate CDDP-induced nephrotoxicity. Accordingly, although cisplatin can cause renal failures through inducing mitochondria dysfunction, oxidative stress, lipid peroxidation and endoplasmic reticulum stress, some CDDP-based nano-carriers have been able to reverse a wide range of these advert effects. Based on the obtained results, it was found that the use of different metallic and polymeric nanoparticles can help renal cells to strengthen their antioxidant systems and stay alive through reducing CDDP-induced ROS generation, inhibiting apoptosis-related pathways and maintaining the integrity of the mitochondrial membrane. For example, nanocurcumin could inhibit oxidative stress and acting as a ROS scavenger. CONPs could reduce lipid peroxidation and pro-inflammatory cytokines. CDDP-loaded silver nanoparticles (AgNPs) could inhibit mitochondria-mediated apoptosis. In addition, tea polyphenol-functionalized SeNPs (Se@TE) NPs could mitigate the increased level of dephosphorylated AKT, phosphorylated p38 MAPK and phosphorylated c-Jun N-terminal kinase (JNK) induced by cisplatin. Moreover, exosomes mitigated cisplatin-induced renal damage through inhibiting Bcl2 and increasing Bim, Bid, Bax, cleaved caspase-9, and cleaved caspase-3. Hence, nanoparticle-based techniques are promising drug delivery systems for cisplatin so that some of them, such as lipoplatins and nanocurcumins, have even reached phases 1–3 trials.

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“…All these factors contribute greatly to their effects in the different fields whether environmentally, industrially, or medically 1 . AgNPs have been proven to have unique antimicrobial toxicity along with other advantageous toxicity like anticancer and catalytic toxicity [2][3][4][5][6] , consequently, they are widely used in fields of water purification, products manufacturing that have antibacterial features, in coatings of medical devices, and as antiseptics 7 .…”

mentioning

confidence: 99%

Dose-dependent biological toxicity of green synthesized silver nanoparticles in rat’s brain

Tareq

Khadrawy

Rageh

et al. 2022

Sci Rep

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Metal nanoparticles, in general, and silver nanoparticles (AgNPs), in particular, have been the focus of numerous studies over the last two decades. Recently, the green synthesis of metal nanoparticles has been favored over chemical synthesis due to its low toxicity and easy preparation. The present study aims to investigate the dose-dependent toxicity of green synthesized AgNPs on rats’ brains. Thirty-four Wistar male rats were divided into four groups. The first, second, and third groups were administered for 14 days with three different doses (0.5, 5, and 10 mg/kg) of AgNPs, respectively. The fourth group, which served as a control group, was given normal saline for the same period. The toxicity of the green synthesized AgNPs on the cortical and hippocampal levels of the oxidative stress markers (MDA, NO, and GSH) as well as the activity of acetylcholinesterase (AchE) and the monoamine neurotransmitters (DA, NE, and 5H-T) were investigated. AgNPs showed minimal oxidative stress in the cortex and hippocampus for the administered doses. However, AgNPs showed an inhibitory effect on AchE activity in a dose-dependent manner and a decrease in the 5H-T and NE levels. The green synthesized AgNPs showed an ultrastructural change in the cellular membranes of the neurons. The green synthesis of AgNPs has reduced their cytotoxic oxidative effects due to their capping with biologically compatible and boosting molecules such as flavonoids. However, another neurotoxicity was observed in a dose-dependent manner.

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Could Cisplatin Loading on Biosynthesized Silver Nanoparticles Improve Its Therapeutic Efficacy on Human Prostate Cancer Cell Line and Reduce Its In Vivo Nephrotoxic Effects?

Cited by 9 publications

References 37 publications

Glucose-Functionalized Silver Nanoparticles as a Potential New Therapy Agent Targeting Hormone-Resistant Prostate Cancer cells

Glucose-Functionalized Silver Nanoparticles as a Potential New Therapy Agent Targeting Hormone-Resistant Prostate Cancer cells

Ameliorative impacts of polymeric and metallic nanoparticles on cisplatin-induced nephrotoxicity: a 2011–2022 review

Dose-dependent biological toxicity of green synthesized silver nanoparticles in rat’s brain

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