Could biological tissue preservation methods change chemical elements proportion measured by energy dispersive X-ray spectroscopy?

Ladeira, Luiz Carlos Maia; Santos, Eliziária Cardoso dos; Valente, Gilmar Edilberto; Silva, Janaína; Santos, Talita Amorim; Maldonado, Izabel Regina dos Santos Costa

doi:10.1007/s12011-019-01909-x

Cited by 10 publications

(4 citation statements)

References 19 publications

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“…Many scientific and therapeutic efforts are underway to identify and combat cancer effectively. For this purpose, some X-ray-based imaging techniques like particle-induced X-ray emission (PIXE), energy-dispersive X-ray spectroscopy (EDX), and X-ray fluorescence microscopy (XRF) were used [3][4][5]. Still, these techniques have low sensitivity and accuracy [6].…”

Section: Introductionmentioning

confidence: 99%

A Review on Laser-Induced Breakdown Spectroscopy in Different Cancers Diagnosis and Classification

et al. 2022

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Due to the new demonstrations of Laser-induced breakdown spectroscopy (LIBS) applicability in a surprisingly wide variety of applications, the use of LIBS as a medical diagnostic tool is steadily gaining momentum. Especially in different cancer diseases, LIBS has the potential to become a fast and valuable analytical tool. We addressed LIBS equipment and quantitative analytical procedures, and signal enhancement techniques for improving element detection. For detailed aspects of applications, we reviewed the recent progress of LIBS in different cancer diseases diagnoses by using different tissues and medical fluid as samples. To fulfill the high demands in the medical industry and overcome the severe tissue sample problem, it is proposed that the chemometric and signal amplification techniques for quantitative analysis should be employed, and robust and effective LIBS devices should be developed. This overview of the different cancers by LIBS is meant to summarize the research performed to date and suggest some suitable advanced chemometrics techniques and effective LIBS devices, if successfully implemented, would be significantly beneficial to the medical field in the future.

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Section: Introductionmentioning

confidence: 99%

A Review on Laser-Induced Breakdown Spectroscopy in Different Cancers Diagnosis and Classification

et al. 2022

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“…The proportion of chemical elements in the testis and the proximal and distal regions of the epididymis ( n = 5/group) was estimated in fragments of frozen organs (Ladeira et al, 2020). Briefly, the fragments were dried in an oven at 60°C for 96 h, coated with carbon (Quorum Q150 T, East Grinstead, West Sussex, UK), and analyzed in a scanning electron microscope (JEOL, JSM-6010LA) with a detector bypass silicon with X-ray radiation.…”

Section: Methodsmentioning

confidence: 99%

Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

et al. 2021

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Green tea is a popular drink used for therapeutic purposes to mitigate the consequences of diabetes. In this study, we aimed at evaluating the potential of green tea infusion to ameliorate structural and enzymatic damages caused by hyperglycemia in the testis and epididymis of Wistar rats. For that, nondiabetic and streptozotocin-induced diabetic rats (negative control and diabetes control, respectively) received 0.6 mL of water by gavage. Another set of diabetic animals received 100 mg/kg of green tea infusion diluted in 0.6 mL of water/gavage (diabetes + green tea) daily. After 42 days of treatment, the testes and epididymides were removed and processed for histopathological analysis, micromineral determination, and enzymatic assays. The results showed that treatment with green tea infusion preserved the testicular and epididymal histoarchitecture, improving the seminiferous epithelium and the sperm production previously affected by diabetes. Treatment with green tea reduced tissue damages caused by this metabolic condition. Given the severity of hyperglycemia, there was no efficacy of the green tea infusion in maintaining the testosterone levels, antioxidant enzyme activity, and microminerals content. Thus, our findings indicate a protective effect of this infusion on histological parameters, with possible use as a complementary therapy for diabetes.

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“…The proportions of zinc (Zn), selenium (Se), copper (Cu), iron (Fe), and manganese (Mn) in the hepatic tissue of 6 animals were evaluated by X-ray dispersive energy spectroscopy (Ladeira et al, 2019). Briefly, liver fragments were dried at 60°C for 96h, coated with evaporated carbon (Quorum Q150 T), and analyzed using a scanning electron microscope (LEO 1430VP; Carl Zeiss) with a X-ray detector system (Tracor TN5502).…”

Section: Mineral Contentmentioning

confidence: 99%

Exposure to Pfaffia glomerata causes oxidative stress and triggers hepatic changes

et al. 2023

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Medicinal plant species are genetically engineered to obtain higher production of biomass and specific secondary metabolites, which can be used in the pharmaceutical industry. The aim of the present study was to evaluate the effect of Pfaffia glomerata (Spreng.) Pedersen tetraploid hydroalcoholic extract on the liver of adult Swiss mice. The extract was prepared from the plant roots and given to the animals by gavage, for 42 days. The experimental groups were treated with water (control), Pfaffia glomerata tetraploid hydroalcoholic extract (100, 200 and 400 mg/kg) and Pfaffia glomerata tetraploid hydroalcoholic extract discontinuously (200 mg/kg). The last group received the extract every 3 days, for 42 days. The oxidative status, mineral dynamics, and cell viability were analysed. The liver weight and the number of viable hepatocytes were reduced, despite the increased cell’s number. Increased levels of malondialdehyde and nitric oxide, and changes in iron, copper, zinc, potassium, manganese and sodium levels were observed. aspartate aminotransferase levels were increased while alanine aminotransferase levels were decreased due to BGEt intake. Our results showed that BGEt induced alterations of oxidative stress biomarkers leading to liver injury, which was associated with a reduction in the number of hepatocytes.

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Could biological tissue preservation methods change chemical elements proportion measured by energy dispersive X-ray spectroscopy?

Cited by 10 publications

References 19 publications

A Review on Laser-Induced Breakdown Spectroscopy in Different Cancers Diagnosis and Classification

A Review on Laser-Induced Breakdown Spectroscopy in Different Cancers Diagnosis and Classification

Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

Exposure to Pfaffia glomerata causes oxidative stress and triggers hepatic changes

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