Cottonseed Flour Effects on Androgen Testicular Content and Serum Levels in Rats

Herrera, J; Montalvo, Irene; González‐Garza, María Teresa; Sotelo, Angela; Bermúdez, José

doi:10.3109/01485018308987476

Archives of Andrology

1983

DOI: 10.3109/01485018308987476

|View full text |Cite

Cottonseed Flour Effects on Androgen Testicular Content and Serum Levels in Rats

J Herrera¹,

Irene Montalvo²,

María Teresa González‐Garza³

et al.

Abstract: Natural gossypol present in the whole cottonseed flour (CSF) has clear antifertility properties, decreasing the number and motility of spermatozoa. In this study the endocrine function of the testes was evaluated, measuring by specific radioimmunoassays the testicular content and the serum levels of androstenedione (A), testosterone (T). and Sa-dihydrotestosterone in male rats fed with different and nontoxic concentrations of CSF along with Purina chow for 4 weeks, followed by 6 weeks of a recovery period. The… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1984

1986

Publication Types

Select...

Article2

Relationship

Self Cite1

Independent1

Authors

Journals

Cited by 2 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A decrease was found in the testicular content of three active androgens as well as a decrease in T serum levels [8].…”

mentioning

confidence: 95%

Modifications in the Testis Steroidogenic Pathways in Rats Fed with Cottonseed Flour

Herrera

Montalvo

Garza

et al. 1984

Archives of Andrology

Self Cite

View full text Add to dashboard Cite

Suppression of the testicular T production by effect of the CSF has been previously reported. Changes in the testicular content of intermediates and precursors of T biosynthesis were determined in rats fed during 28 days with diets containing 25, 35 and 50% of CSF and compared with a defated cottonseed flour diet group and a control Purina-chow diet group. Also described were modifications during a six wk post CSF diet period. The results showed a diminution in pregnenolone and progesterone testicular content to a third and a half of the control values (21.5 and 19.3 pg/mg of protein respectively). Other compounds in the .A5 pathway, 17 hidroxypregnenolone and dehydroepiandrosterone were undetectable since the 35% CSF diet, the 17 hidroxyprogesterone diminished to undetectable values only with the maximal (50%) CSF content diet. During the post CSF diet period all the steroids showed a tendency toward the control values. The 25% CSF content diet group reached the initial contents; other groups presented different recovery degrees. The most affected was the 50% diet group with a direct effect of the CSF on the testicular androgen steroidogenesis affecting both the .A4 and the .As pathways.Key Words: Gossypol, testis, steroidogenic pathways INTRODUCTIONThe gossypol effects on the morphology and motility of spermatozoa are well documented [4, 9, 113, however, its effects on the testicular endocrine function is controversial, since initial reports as well as other papers have found no changes in the plasmatic hormones [ 1, 113. Lin et al. showed a significant decrease of plasmatic testosterone (T) levels with a possible blockade of testicular steroidogenesis [ 101.The antifertility effect, similar to that of gossypol, was demonstrated in rats fed with whole cottonseed flour (CSF) supplemented diets [12]. A decrease was found in the testicular content of three active androgens as well as a decrease in T serum levels [8].To correlate those results with a blockade in the androgen steroidogenic pathways the testicular content of As precursors pregnenolone (Preg), 17 hidroxypregnenolone (170HPreg), dehydroepiandrosterone (DHEA); A4 intermediates-progesterone (Prog), 17 hidroxyprogesterone (170HPrbg), and the active androgens-androstenedione (A), testosterone (T) and 5 a dihydrotestosterone (DHT) were measured by specific radioimmunoassays (RIAs) in rats fed with CSF additioned diets. MATERIALS AND METHODSThe CSF diets were prepared and evaluated as reported [ 121, the experimental procedure was as follows: Diets containing 100% defated CSF or 25, 35 and 50% whole CSF and a control Purina chow diet were used to feed 5 groups with 10 adult male Sprague-Dawley rats each during 4 wk. At the end of the exposure period 4 animals from each group were sacrificed by decapitation; the remaining animals were fed a purina chow diet during 3 to 6 wks to allow their recovery prior to their sacrifice. The testes were separated. decapsulated, and homogenized in buffered saline solution to a final volume of 5 mi, aliquots of homogenates...

show abstract

“…A decrease was found in the testicular content of three active androgens as well as a decrease in T serum levels [8].…”

mentioning

confidence: 95%

Modifications in the Testis Steroidogenic Pathways in Rats Fed with Cottonseed Flour

Herrera

Montalvo

Garza

et al. 1984

Archives of Andrology

Self Cite

View full text Add to dashboard Cite

show abstract

Serum testosterone, testicular connective tissue protein and testicular histology in rats treated with hyperbaric oxygen

Nakada

Saito

et al. 1986

International Urology and Nephrology

View full text Add to dashboard Cite

This study was done to ascertain whether hyperbaric oxygenation really affects testicular activity in rats. We evaluated serum testosterone concentration, testicular content of connective tissue protein and histological analysis of testes in each rat treated with 2 or 3 atmospheric pressures associated with oxygen saturation, together or singly for 90 minutes daily for 58 days. There were no significant differences in serum testosterone concentrations between the examined groups. The amounts of collagen, non-collagenous protein or elastin in testis were similar in all groups except one set of determinations. In addition, no prominent histological differences were noted in the testes of the animals. Thus, the pressure environment of this study, which can be regarded as almost the same as that of the hyperbaric oxygenation chamber in clinical use, appears to be nontoxic to rat testes.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Cottonseed Flour Effects on Androgen Testicular Content and Serum Levels in Rats

Cited by 2 publications

References 8 publications

Modifications in the Testis Steroidogenic Pathways in Rats Fed with Cottonseed Flour

Modifications in the Testis Steroidogenic Pathways in Rats Fed with Cottonseed Flour

Serum testosterone, testicular connective tissue protein and testicular histology in rats treated with hyperbaric oxygen

Contact Info

Product

Resources

About