Cotton Fiber Growth in Planta and in Vitro. Models for Plant Cell Elongation and Cell Wall Biogenesis

Kim, H. J.

doi:10.1104/pp.127.4.1361

Cited by 170 publications

(205 citation statements)

References 18 publications

(20 reference statements)

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“…AOC is a key enzyme in the biosynthesis of JA [37]. Plant hormones such as indole-3-acetic acid (IAA), gibberellic acid (GA3) and brassinosteroid (BR) are known to play important roles in cotton fiber initiation [38,39], and these results suggest that JA may also be involved in the progression of fiber initiation.…”

Section: Resultsmentioning

confidence: 99%

“…In Medicago truncatula , suppressing MtAOC1 expression in roots lowered JA levels significantly [37], which highlighted the importance of AOC in JA biosynthesis. Genes encoding enzymes involved in JA biosynthesis generally induced by JA in Arabidopsis and tomato, indicating feed-forward regulation [38, 57]. JA, OPDA and their derivatives can induce AOC expression in Arabidopsis [58].…”

Section: Discussionmentioning

confidence: 99%

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Comparative Transcriptomics Reveals Jasmonic Acid-Associated Metabolism Related to Cotton Fiber Initiation

Wang

Zhu

et al. 2015

PLoS ONE

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Analysis of mutants and gene expression patterns provides a powerful approach for investigating genes involved in key stages of plant fiber development. In this study, lintless-fuzzless XinWX and linted-fuzzless XinFLM with a single genetic locus difference for lint were used to identify differentially expressed genes. Scanning electron microscopy showed fiber initiation in XinFLM at 0 days post anthesis (DPA). Fiber transcriptional profiling of the lines at three initiation developmental stages (-1, 0, 1 DPA) was performed using an oligonucleotide microarray. Loop comparisons of the differentially expressed genes within and between the lines was carried out, and functional classification and enrichment analysis showed that gene expression patterns during fiber initiation were heavily associated with hormone metabolism, transcription factor regulation, lipid transport, and asparagine biosynthetic processes, as previously reported. Further, four members of the allene-oxide cyclase (AOC) family that function in jasmonate biosynthesis were parallel up-regulation in fiber initiation, especially at -1 DPA, compared to other tissues and organs in linted-fuzzed TM-1. Real time-quantitative PCR (RT-qPCR) analysis in different fiber mutant lines revealed that AOCs were up-regulated higher at -1 DPA in lintless-fuzzless than that in linted-fuzzless and linted-fuzzed materials, and transcription of the AOCs was increased under jasmonic acid (JA) treatment. Expression analysis of JA biosynthesis-associated genes between XinWX and XinFLM showed that they were up-regulated during fiber initiation in the fuzzless-lintless mutant. Taken together, jasmonic acid-associated metabolism was related to cotton fiber initiation. Parallel up-regulation of AOCs expression may be important for normal fiber initiation development, while overproduction of AOCs might disrupt normal fiber development.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomics Reveals Jasmonic Acid-Associated Metabolism Related to Cotton Fiber Initiation

Wang

Zhu

et al. 2015

PLoS ONE

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“…Microscopic observation further confirmed that the cell walls of SG20-1 and BG2i-2 mature fibers were thicker (5.4% and 6.6%, respectively) than the wild-type control (Figure 2B and D). Considered that most of cell walls of mature cotton fibers consisted of secondary cell wall [2], it was reasonable that the fineness increase in SG20-1 and BG2i-2 fibers might be mainly attributed to promotion of secondary cell wall deposition. To prove this hypothesis, we determined the cellulose contents in fibers, and found that the contents of SG20-1 and BG2i-2 fibers were significantly higher than the control (Figure 2F).…”

Section: Resultsmentioning

confidence: 99%

Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers

et al. 2014

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Bioactive gibberellins (GAs) comprise an important class of natural plant growth regulators and play essential roles in cotton fiber development. To date, the molecular base of GAs' functions in fiber development is largely unclear. To address this question, the endogenous bioactive GA levels in cotton developing fibers were elevated by specifically up-regulating GA 20-oxidase and suppressing GA 2-oxidase via transgenic methods. Higher GA levels in transgenic cotton fibers significantly increased micronaire values, 1000-fiber weight, cell wall thickness and cellulose contents of mature fibers. Quantitative RT-PCR and biochemical analysis revealed that the transcription of sucrose synthase gene GhSusA1 and sucrose synthase activities were significantly enhanced in GA overproducing transgenic fibers, compared to the wild-type cotton. In addition, exogenous application of bioactive GA could promote GhSusA1 expression in cultured fibers, as well as in cotton hypocotyls. Our results suggested that bioactive GAs promoted secondary cell wall deposition in cotton fibers by enhancing sucrose synthase expression.

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“…Finally, in addition to being an excellent system for studying cell growth, cotton fibre is also the most important source of cellulose for the textile industry, with >94% of mature fibre dry weight being cellulose, a β-1,4 polymer of glucose (Basra and Malik 1984;Delmer 1999). Hence, understanding the mechanisms underlying rapid cell elongation and cellulose production, two key processes that determine cotton fibre quality and yield, could lead to the identification of target genes for genetic engineering of fibre development for the improvement of cotton productivity (John 1997;Kim and Triplett 2001;Ruan 2005).…”

Section: Introductionmentioning

confidence: 99%

Rapid cell expansion and cellulose synthesis regulated by plasmodesmata and sugar: insights from the single-celled cotton fibre

Ruan¹

2007

Functional Plant Biol.

100

102

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Abstract.Higher plants comprise mixtures of some 40 different cell types, and this often complicates the interpretation of data obtained at the tissue level. Studies for a given cell type may provide novel insights into the mechanisms underlying defined cellular and developmental processes. In this regard, the cotton fibre represents an excellent single-cell model to study the control of rapid cell elongation and cellulose synthesis. These single cells, initiated from the ovule epidermis at anthesis, typically elongate to ∼3-5 cm in the tetraploid species before they switch to intensive secondary cell wall cellulose synthesis. By maturity, more than 94% of fibre weight is cellulose. To unravel the mechanisms of fibre elongation and cellulose synthesis, two hypotheses have been examined: (a) that sucrose degradation and utilisation mediated by sucrose synthase (Sus) may play roles in fibre development and (b) that symplastic isolation of the fibre cells may be required for their rapid elongation. Reverse genetic and biochemical analyses have revealed the critical role that Sus plays in fibre initiation and early elongation. Late in development, plasma-membrane and cell wall association of Sus protein seems to be involved in rapid cellulose synthesis. Cell biology and gene expression studies showed a temporary closure of fibre plasmodesmata (PD), probably due to the deposition of callose, at the rapid phase of elongation. The duration of the PD closure correlates positively with the final fibre length attained. These data support the view that PD closure may be required for fibres to achieve extended elongation. The branching of PD towards the secondary cell wall stage is postulated to function as a molecule sieve for tight control of macromolecule trafficking into fibres to sustain intensive cellulose synthesis.

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Cotton Fiber Growth in Planta and in Vitro. Models for Plant Cell Elongation and Cell Wall Biogenesis

Cited by 170 publications

References 18 publications

Comparative Transcriptomics Reveals Jasmonic Acid-Associated Metabolism Related to Cotton Fiber Initiation

Comparative Transcriptomics Reveals Jasmonic Acid-Associated Metabolism Related to Cotton Fiber Initiation

Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers

Rapid cell expansion and cellulose synthesis regulated by plasmodesmata and sugar: insights from the single-celled cotton fibre

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