Comparative Transcriptomics Reveals Jasmonic Acid-Associated Metabolism Related to Cotton Fiber Initiation

Abstract

Analysis of mutants and gene expression patterns provides a powerful approach for investigating genes involved in key stages of plant fiber development. In this study, lintless-fuzzless XinWX and linted-fuzzless XinFLM with a single genetic locus difference for lint were used to identify differentially expressed genes. Scanning electron microscopy showed fiber initiation in XinFLM at 0 days post anthesis (DPA). Fiber transcriptional profiling of the lines at three initiation developmental stages (-1, 0, 1 DPA) was performed using an oligonucleotide microarray. Loop comparisons of the differentially expressed genes within and between the lines was carried out, and functional classification and enrichment analysis showed that gene expression patterns during fiber initiation were heavily associated with hormone metabolism, transcription factor regulation, lipid transport, and asparagine biosynthetic processes, as previously reported. Further, four members of the allene-oxide cyclase (AOC) family that function in jasmonate biosynthesis were parallel up-regulation in fiber initiation, especially at -1 DPA, compared to other tissues and organs in linted-fuzzed TM-1. Real time-quantitative PCR (RT-qPCR) analysis in different fiber mutant lines revealed that AOCs were up-regulated higher at -1 DPA in lintless-fuzzless than that in linted-fuzzless and linted-fuzzed materials, and transcription of the AOCs was increased under jasmonic acid (JA) treatment. Expression analysis of JA biosynthesis-associated genes between XinWX and XinFLM showed that they were up-regulated during fiber initiation in the fuzzless-lintless mutant. Taken together, jasmonic acid-associated metabolism was related to cotton fiber initiation. Parallel up-regulation of AOCs expression may be important for normal fiber initiation development, while overproduction of AOCs might disrupt normal fiber development.

Histone Deacetylase (HDAC) Gene Family in Allotetraploid Cotton and Its Diploid Progenitors: In Silico Identification, Molecular Characterization, and Gene Expression Analysis under Multiple Abiotic Stresses, DNA Damage and Phytohormone Treatments

“…Interestingly, we observed that the expression of GhHDT1502, GhHDT1503, and GhHDT1504 gradually decreased with the different stages of cotton fiber development (0-25 DPA), suggesting the dynamic levels of histone acetylation mediated by G. hirsutum HDACs in cotton fiber development. Fiber development is also regulated by phytohormones, including abscisic acid, auxin, gibberellic acid, ethylene, and jasmonic acid [5,[43][44][45][46]. Phytohormones have been reported to alter the chromatin structure through the regulation of chromatin modifiers [47], suggesting a potential crosstalk between phytohormones and histone acetylation.…”

Genome-wide comparative and evolutionary analysis of Calmodulin-binding Transcription Activator (CAMTA) family in Gossypium species

“…Additionally, CAMTAs are known to interact with various phytohormones including ethylene 15 , ABA 19 , salicylic acid 21 , auxin 13 , and jasmonate 21 . These phytohormones also play critical roles in cotton fiber development 53 , 54 . Thus identification of genes belonging to these phytohormone categories in PCoEGs and NCoEGs (Fig.…”

Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)

“…Also, JA-associated metabolism is related to cotton fiber initiation 59 . In our work, we found that most of the GhJAZ genes could be induced by JA in the ovules during fiber initiation.…”