“…[28][29][30][31] Hence, there is a great need for an ecofriendly, fast, easy, and selective procedure for the detection of SFB alone or in the presence of other antiviral drugs, which does not require a long extraction, complicated equipment or sample collection procedures, and without expensive solvents. Many analytical methods were established for SFB determination such as the most recent HPLC, [32][33][34] LC-MS/MS, [35][36][37] spectrophotometric, 26,38 and HPTLC. 39 These methods have a very high accuracy, however, the majority of them require a lot of complicated extraction procedures in addition to requiring highly expensive instrumentation and a high rate of solvent consumption.…”