Simple simultaneous determination of moxifloxacin and metronidazole in complex biological matrices

Abstract

A simple, sensitive and rapid RP-HPLC method is presented, for the first time, for the simultaneous determination of moxifloxacin hydrochloride and metronidazole in different biological fluids including saliva and plasma without any matrix interference.

Eco‐friendly micellar HPLC approach for simultaneous estimation of combination therapy for hidradenitis suppurativa: Applications to spiked human plasma and different dosage forms

“…Based on the previously discussed parameters, five assessment metrics, namely the analytical GREEnness calculator (AGREE), [27] the green analytical procedure index (GAPI), [28] the National Environmental Index (NEMI), Raynie and Driver, [29] and the analytical Eco-scale, [30] were used to evaluate the procedures that were followed through this approach and were compared with previously reported ones. [7][8][9][10] First, the GAPI had a score represented in the shape of a colored pictogram, which included five pentagrams with three colors (green, yellow, and red) to quantify and evaluate the environmental impact of the developed analytical method as presented in Table 10. Green, yellow, and red depict low, medium, and major ecological consequences.…”

“…To prepare the standard working solutions, 0.5 mL of each stock solution was transferred in a 10 mL volumetric flask, and the remaining volume was filled with mobile phase to a final concentration of 50 μg mL −1 . Reported approach [7] Reported approach [8] Reported approach [9] Reported approach [10] Reagents Ammonium acetate…”

“…[2] A literature review reported few methods for simultaneously determining the cited drugs, either in pure form or plasma. For the analysis of RIF and MXF simultaneously, two high performance liquid chromatography-ultraviolet (HPLC) techniques were documented, [7,8] whereas for the determination of MXF and MTZ, two HPLC [9,10] and one electrochemistry-mass spectrometry [11] methods were reported. Also, one spectrofluorimetric method for RIF and MTZ assay [12] was described.…”

Greener and Whiter Analytical Chemistry Using Cyrene as a More Sustainable and Eco-Friendlier Mobile Phase Constituent in Chromatography

“…Several RP-HPLC methods were reported for the assay of metronidazole and moxifloxacin alone or in combination with other drugs using either acetonitrile or methanol in the mobile phase [46][47][48][49][50]. The simultaneous HPLC determination of metronidazole and moxifloxacin developed by El-Yazbi et al [51] using acetonitrile was considered a reference method for further comparison. The main aim of this research was to consider the potential applicability of Cyrene as a mobile phase component in simultaneous chromatographic separation and determination of metronidazole and moxifloxacin.…”

“…Several RP-HPLC methods were reported for the assay of metronidazole and moxifloxacin alone or in combination with other drugs using either acetonitrile or methanol in the mobile phase [46][47][48][49][50]. The simultaneous HPLC determination of metronidazole and moxifloxacin developed by El-Yazbi et al [51] using acetonitrile was considered a reference method for further comparison.…”