Corynoline protects lipopolysaccharide‐induced mastitis through regulating AKT/GSK3β/Nrf2 signaling pathway

Abstract

Inflammation has been known to be involved in the pathogenesis of mastitis. And anti-inflammatory agent is proposed to be a possible efficient therapeutic strategy for mastitis. Corynoline, a bioactive compound extracted from Corydalis bungeana Turcz., has been reported to have anti-inflammatory effect. However, whether corynoline has protective effect against mastitis remains unclear. The aim of this study was to evaluate the protective effect of corynoline on LPS-induced mastitis in mice.Inflammatory cytokine production was measured by ELISA. The proteins of signaling pathways were detected by western blot analysis. The results showed that treatment of corynoline at the doses of 15, 30, and 60 mg/kg significantly attenuated LPSinduced pathological damage of mammary tissues. Corynoline also ameliorated LPSinduced MPO activity, MDA content, and inflammatory cytokine TNF-α and IL-1β production in mammary tissues. LPS-induced NF-κB activation was inhibited by corynoline. Furthermore, our results showed corynoline significantly increased the expression of Nrf2 and the phosphorylation levels of AKT and GSK3β. In conclusion, our results indicated that corynoline protected against LPS-induced mastitis through regulating AKT/GSK3β/Nrf2 signaling pathway, which subsequently led to the inhibition of NF-κB and inflammatory response.

Targeted activation of Nrf2/HO‐1 pathway by Corynoline alleviates osteoporosis development

“…Furthermore, we demonstrated the decreased Nrf2 expression by ML385, followed by the significantly weakened efficacy of Corynoline. Wu et al proposed that Corynoline activated the Nrf2 pathway by promoting the phosphorylation of AKT and GSK3β (Wu et al, 2021 ). Liu et al reported that Corynoline exerted its effects by manipulating the Nrf2 pathway and suppressing NF‐kB activation (Liu et al, 2018 ).…”

“…Li et al ( 2022 ) demonstrate this component to alleviate osteoarthritis via the Nrf2/NF‐kB pathway. In addition, Wu et al reported that it could protect lipopolysaccharide‐induced mastitis by manipulating the Nrf2 signaling pathway (Wu et al, 2021 ). However, the specific mechanism that Corynoline ameliorates oxidative stress‐induced damage remains unclear.…”

Gossypin exert lipopolysaccharide induced lung inflammation via alteration of Nrf2/HO‐1 and NF‐κB signaling pathway

“…Following the exposure of lung tissues to foreign substances like LPS (endotoxins), the immune system reacts with the proinflammatory cytokines. Cytokines are low molecular weight soluble glycoproteins that are secreted by various cells and tissues 58 . This activates an immune response, causing inflammation as a way to eliminate the foreign substances and protect the tissues.…”

“…Cytokines are low molecular weight soluble glycoproteins that are secreted by various cells and tissues. 58 This activates an immune response, causing inflammation as a way to eliminate the foreign substances and protect the tissues.…”

Corynoline protects against zearalenone‐induced liver injury by activating the SIRT1/Nrf2 signaling pathway

“…The doses of corynoline used in this study were based on previous studies. [14,15] The mice of corynoline (60mg/kg)+EX-527 (10 mg/kg) + ZEA group were given corynoline (60 mg/kg) and EX-527 (10 mg/kg) by intraperitoneal injection for 5 days and then administrated ZEA. The mice were ultimately nesthetized and killed for subsequent experiments.…”