Corynebacterium vaginale vaginitis

Lewis, Jay F.; O’Brien, Susan; Ural, Unal M.; Burke, Thomas F.

doi:10.1016/0002-9378(72)90534-0

Cited by 26 publications

(14 citation statements)

References 20 publications

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“…Since Gardner and Dukes (7) first isolated Gardnerella (Haemophilus) vaginalis from women with nonspecific vaginitis (NSV) in 1955, there has been controversy over the role of this organism in NSV. Some investigators (6,13) have noticed no difference in G. vaginalis prevalence between vaginal fluid of women with vaginitis and vaginal fluid of women without vaginitis, whereas others (1,12,14) have isolated G. vaginalis more often from women with NSV than from normal control women. Variables affecting studies of the correlation of G. vaginalis with NSV may include criteria used to diagnose NSV, comparability of NSV patients with controls, media used for isolation of G. vaginalis, and criteria used for identification of this organism. Recent taxonomic studies (10, 15) have proposed placement of this organism in a new genus, Gardnerella, and have clarified the characteristics of this organism, making more-accurate identification possible.…”

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confidence: 99%

Selective differential human blood bilayer media for isolation of Gardnerella (Haemophilus) vaginalis

Totten¹,

Amsel²,

Hale³

et al. 1982

J Clin Microbiol

172

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New selective and differential human blood bilayer agar media with Tween 80 (HBT medium) or without Tween 80 (HB medium), developed for the isolation of Gardnerella (Haemophilus) vaginalis, permitted significantly higher G. vaginalis isolation rates than have been obtained for other media used for this purpose. HB medium consists of a basal layer of Columbia agar base containing colistin and naladixic acid with added amphotericin B and an overlayer of the same composition plus 5% human blood. HBT agar also contains Proteose Peptone No. 3 (Difco Laboratories) and Tween 80 in the basal layer and the overlayer. Both Tween 80 and the bilayer composition enhanced G. vaginalis production of human blood hemolysis, permitting detection of this organism even in the presence of heavy growth of other vaginal flora. The use of HB or HBT medium thus permitted the demonstration that G. vaginalis was present in vaginal fluid from a large percentage (up to 68%) of normal women. However, the concentration of G. vaginalis was found by semiquantitative analysis to be significantly higher in vaginal fluid from women with nonspecific vaginitis than in fluid from normal women.

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confidence: 99%

Selective differential human blood bilayer media for isolation of Gardnerella (Haemophilus) vaginalis

Totten¹,

Amsel²,

Hale³

et al. 1982

J Clin Microbiol

172

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“…tNo of strains with more than 20% adhesion in patients with clue cells: 21-40% (13), 41-60% (8), 61-80%(7) and 81-100%(11); and in patients without clue cells: 21-40% (2), 41-60% (1), 61-80% (2), and 81-100%(2).…”

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confidence: 99%

In vitro adhesiveness and biotype of Gardnerella vaginalis strains in relation to the occurrence of clue cells in vaginal discharges.

Scott¹,

Smyth²,

Keane³

1987

Sexually Transmitted Infections

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“…Gardnerella vaginalis has been implicated as the etiological agent of nonspecific vaginitis (NSV) in numerous studies (1,11,13,15,19,20) foliowing the initial report by Gardner and Dukes (6). However, the significance of the isolation of this organism from the lower genital tract has been controversial because asymptomatic women are frequently found to have positive cultures for G. vaginalis (5,12,14).…”

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Semiquantitative culture of Gardnerella vaginalis in laboratory determination of nonspecific vaginitis

Ratnam¹,

Fitzgerald²

1983

J Clin Microbiol

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To evaluate the usefulness of quantitative cultures of Gardnerella vaginalis in the laboratory determination of nonspecific vaginitis, the actual and relative numbers of G. vaginalis in genital cultures of a general patient population were assessed semiquantitatively, and the laboratory results were then correlated with the clinical findings. Of the 1,585 women studied, 417 (26.3%) yielded G. vaginalis in culture. Of these, only 113 (27.1%) were found to have symptoms and signs consistent with nonspecific vaginitis. G. vaginalis was obtained in pure or predominant growth from 87 of 100 consecutive cases with nonspecific vaginitis and 32 of 100 consecutive cases without the symptoms or signs of vaginitis (P less than 0.001). Hence, the positive predictive value of isolation of G. vaginalis in pure and predominant growths was determined to be 73% (87 of 119). Conversely, G. vaginalis was isolated in mixed or light growth significantly more often from asymptomatic women than from symptomatic patients, i.e., 68 versus 13 cases. Therefore, the negative predictive value of isolation of G. vaginalis in mixed and light growths was found to be 84% (68 of 81). Quantitation of the relative amount of G. vaginalis growth had higher predictive values as compared with the assessment of G. vaginalis growth alone. We conclude that quantitative culture of G. vaginalis is essential to obtain maximum reliability of culture results in the laboratory determination of nonspecific vaginitis. Although quantitated cultures of G. vaginalis have high predictive values, laboratory results must be interpreted in conjunction with the clinical findings.

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Corynebacterium vaginale vaginitis

Cited by 26 publications

References 20 publications

Selective differential human blood bilayer media for isolation of Gardnerella (Haemophilus) vaginalis

Selective differential human blood bilayer media for isolation of Gardnerella (Haemophilus) vaginalis

In vitro adhesiveness and biotype of Gardnerella vaginalis strains in relation to the occurrence of clue cells in vaginal discharges.

Semiquantitative culture of Gardnerella vaginalis in laboratory determination of nonspecific vaginitis

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