Corynebacterium urinapleomorphum sp. nov., a new bacterial species isolated from human urine sample

Niang, El Hadji Amadou; Lo, Cheikh Ibrahima; Morand, Aurélie; Ndongo, S.; Raoult, Didier; Fournier, Pierre‐Edouard; Fénollar, Florence

doi:10.1016/j.nmni.2019.100576

Cited by 11 publications

(4 citation statements)

References 17 publications

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“…Of the 29 species that were not previously detected in humans, 11 were previously recognized taxa. Among the main discoveries, the present study was able to isolate 18 different new species and genera ( Cresci et al, 2016 ; Morand et al, 2016a , b , c ; Brahimi et al, 2017a , b , c , d ; Niang et al, 2019a , b ; Yimagou et al, 2019 ) ( Table 2 ). Of these, seven (39%) were detected in several projects.…”

Section: Resultsmentioning

confidence: 76%

Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut

et al. 2020

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Human urine was considered sterile for a long time. However, 416 species have been previously cultured, including only 40 anaerobic species. Here, we used culturomics, particularly those targeting anaerobes, to better understand the urinary microbiota. By testing 435 urine samples, we isolated 450 different bacterial species, including 256 never described in urine of which 18 were new species. Among the bacterial species identified, 161 were anaerobes (35%). This study increased the known urine repertoire by 39%. Among the 672 bacterial species isolated now at least once from urine microbiota, 431 (64.1%) were previously isolated from gut microbiota, while only 213 (31.7%) were previously isolated from vagina. These results suggest that many members of the microbiota in the urinary tract are in fact derived from the gut, and a paradigm shift is thus needed in our understanding.

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Section: Resultsmentioning

confidence: 76%

Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut

et al. 2020

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“…More than 50% of the strains in groups A, B, C, F and K were isolated from humans, mostly associated with clinical infections ( Figures 2 , 3A ; Supplementary Table 1 ). All five species in group C were isolated from humans with clinical associations ( Supplementary Table 1 ; Figure 3A ) except for Corynebacterium urinipleomorphum , which was isolated from urine of an infant with rotavirus gastroenteritis ( Niang et al, 2019 ). Corynebacterium urinipleomorphum strains have also been isolated from other clinical sources, e.g., from a patient with gallbladder infection along with other bacterial species ( Backert et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Phylogenomic Reappraisal of Fatty Acid Biosynthesis, Mycolic Acid Biosynthesis and Clinical Relevance Among Members of the Genus Corynebacterium

et al. 2021

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The genus Corynebacterium encompasses many species of biotechnological, medical or veterinary significance. An important characteristic of this genus is the presence of mycolic acids in their cell envelopes, which form the basis of a protective outer membrane (mycomembrane). Mycolic acids in the cell envelope of Mycobacterium tuberculosis have been associated with virulence. In this study, we have analysed the genomes of 140 corynebacterial strains, including representatives of 126 different species. More than 50% of these strains were isolated from clinical material from humans or animals, highlighting the true scale of pathogenic potential within the genus. Phylogenomically, these species are very diverse and have been organised into 19 groups and 30 singleton strains. We find that a substantial number of corynebacteria lack FAS-I, i.e., have no capability for de novo fatty acid biosynthesis and must obtain fatty acids from their habitat; this appears to explain the well-known lipophilic phenotype of some species. In most species, key genes associated with the condensation and maturation of mycolic acids are present, consistent with the reports of mycolic acids in their species descriptions. Conversely, species reported to lack mycolic acids lacked these key genes. Interestingly, Corynebacterium ciconiae, which is reported to lack mycolic acids, appears to possess all genes required for mycolic acid biosynthesis. We suggest that although a mycolic acid-based mycomembrane is widely considered to be the target for interventions by the immune system and chemotherapeutics, the structure is not essential in corynebacteria and is not a prerequisite for pathogenicity or colonisation of animal hosts.

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“…Recently new species of Corynebacterium have been identified from human urine samples including C. urinapleomorphum Marseille-P2799 T [15] and C. phoceense MC1 T [16]. Neither has been found to be the causative agent of infection.…”

Section: Introductionmentioning

confidence: 99%

Corynebacterium phoceense, resident member of the urogenital microbiota?

et al. 2021

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Corynebacterium phoceense is a Gram-positive species previously isolated from human urine. Although other species from the same genus have been associated with urinary tract infections, C. phoceense is currently believed to be a non-pathogenic member of the urogenital microbiota. Prior to our study, only two isolates were described in the literature, and very little is known about the species. Here, we describe C. phoceense UFMG-H7, the first strain of this species isolated from the urine of healthy cattle. The genome for this isolate was produced and compared to the two other publicly available C. phoceense as well as other Corynebacterium genome assemblies. Our in-depth genomic analysis identified four additional publicly available genome assemblies that are representatives of the species, also isolated from the human urogenital tract. Although none of the strains have been associated with symptoms or disease, numerous genes associated with virulence factors are encoded. In contrast to related Corynebacterium species and Corynebacterium species from the bovine vaginal tract, all C. phoceense strains examined code for the SpaD-type pili suggesting adherence is essential for its persistence within the urinary tract. As the other C. phoceense strains analysed were isolated from the human urogenital tract, our results suggest that this species may be specific to this niche.

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Corynebacterium urinapleomorphum sp. nov., a new bacterial species isolated from human urine sample

Abstract: Corynebacterium urinapleomorphum sp. nov. stain Marseille-P2799 T (= CSURP2799; = DSM103272) is a new species from the order Corynebacteriales that was isolated from urine of a 2-month-old child with gastroenteritis.

Cited by 11 publications

References 17 publications

Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut

Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut

Phylogenomic Reappraisal of Fatty Acid Biosynthesis, Mycolic Acid Biosynthesis and Clinical Relevance Among Members of the Genus Corynebacterium

Corynebacterium phoceense, resident member of the urogenital microbiota?

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