Corynebacterium species nasopharyngeal carriage in asymptomatic individuals aged ≥ 65 years in Germany

Teutsch, Barbara; Berger, Anja; Marosevic, Durdica; Schönberger, Katharina; Lâm, Thiên-Trí; Hubert, Kerstin; Beer, Steffi; Wienert, Peter; Ackermann, Nikolaus; Claus, Heike; Drayß, Maria; Thiel, Kathrin; Linden, Mark van der; Vogel, Ulrich; Sing, Andreas

doi:10.1007/s15010-017-1017-0

Cited by 10 publications

(10 citation statements)

References 21 publications

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“…Study centres were the Wuerzburg region (Bavaria) and the greater Aachen region (North Rhine Westphalia) in Germany. The German National Consulting Laboratory for Diphtheria also investigated asymptomatic carriage of Corynebacterium species, which has been published elsewhere [27]. We addressed community-dwellers attending cafés or clubs for older people as well as residents of sheltered housing and care facilities.…”

Section: Methodsmentioning

confidence: 99%

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Asymptomatic carriage of Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, Group A Streptococcus and Staphylococcus aureus among adults aged 65 years and older

et al. 2019

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ObjectiveThe aim of this study was to determine the prevalence of Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, group A Streptococcus (GAS), and Staphylococcus aureus in asymptomatic elderly people and to unravel risk factors leading to colonization.MethodsA multi-centre cross-sectional study was conducted including 677 asymptomatic adults aged 65 years or more, living at home or in nursing homes. Study areas were Greater Aachen (North-Rhine-Westphalia) and Wuerzburg (Bavaria), both regions with medium to high population density. Nasal and oropharyngeal swabs as well as questionnaires were collected from October 2012 to May 2013. Statistical analysis included multiple logistic regression models.ResultsThe carriage rate was 1.9% ([95%CI: 1.0–3.3%]; 13/677) for H. influenzae, 0.3% ([95%CI: 0–1.1%]; 2/677) for N. meningitidis and 0% ([95% CI: 0–0.5%]; 0/677) for S. pneumoniae and GAS. Staphylococcus aureus was harboured by 28.5% of the individuals ([95% CI: 25.1–32.1%]; 193/677) and 0.7% ([95% CI: 0.2–1.7%]; 5/677) were positive for methicillin-resistant S. aureus. Among elderly community-dwellers colonization with S. aureus was significantly associated with higher educational level (adjusted OR: 1.905 [95% CI: 1.248–2.908]; p = 0.003). Among nursing home residents colonization was associated with being married (adjusted OR: 3.367 [1.502–7.546]; p = 0.003).ConclusionThe prevalence of N. meningitidis, H. influenzae, S. pneumoniae and GAS was low among older people in Germany. The S. aureus rate was expectedly high, while MRSA was found in less than 1% of the individuals.

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Section: Methodsmentioning

confidence: 99%

“…In this context, nasopharyngeal carriage of Corynebacterium sp. has also been assessed and reported previously [27].…”

Section: Introductionmentioning

confidence: 99%

Asymptomatic carriage of Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, Group A Streptococcus and Staphylococcus aureus among adults aged 65 years and older

et al. 2019

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“…Identification of Corynebacterium species negatively associated with S. pneumoniae colonization Given the diversity of Corynebacterium species isolated from the human upper respiratory tract [56][57][58], we sought to determine if specific species accounted for the negative association with S. pneumoniae colonization. We used BLAST searches to identify the species or group of closely related species (supraspecies) corresponding to each of the 279 Corynebacterium amplicon sequence variants (ASVs) identified in infant nasopharyngeal samples [59].…”

Section: Environmental Exposures Influence Nasopharyngeal Microbiome ...mentioning

confidence: 99%

Non-diphtheriae Corynebacterium species are associated with decreased risk of pneumococcal colonization during infancy

Kelly

Plunkett

et al. 2021

The ISME Journal

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Streptococcus pneumoniae (pneumococcus) is a leading cause of severe infections among children and adults. Interactions between commensal microbes in the upper respiratory tract and S. pneumoniae are poorly described. In this study, we sought to identify interspecies interactions that modify the risk of S. pneumoniae colonization during infancy and to describe development of the upper respiratory microbiome during infancy in a sub-Saharan African setting. We collected nasopharyngeal swabs monthly (0-6 months of age) or bimonthly (6-12 months of age) from 179 mother-infant dyads in Botswana. We used 16S ribosomal RNA gene sequencing to characterize the nasopharyngeal microbiome and identified S. pneumoniae colonization using a species-specific PCR assay. We detect S. pneumoniae colonization in 144 (80%) infants at a median age of 71 days and identify a strong negative association between the relative abundance of the bacterial genera Corynebacterium within the infant nasopharyngeal microbiome and the risk of S. pneumoniae colonization. Using in vitro cultivation experiments, we demonstrate growth inhibition of S. pneumoniae by secreted factors from strains of several Corynebacterium species isolated from these infants. Finally, we demonstrate that antibiotic exposures and the winter season are associated with a decline in the relative abundance of Corynebacterium within the nasopharyngeal microbiome, while breastfeeding is associated with an increase in the Corynebacterium relative abundance. Our findings provide novel insights into the interspecies interactions that contribute to colonization resistance to S. pneumoniae and suggest that the nasopharyngeal microbiome may be a previously unrecognized mechanism by which environmental factors influence the risk of pneumococcal infections during childhood. Moreover, this work lays the foundation for future studies seeking to use targeted manipulation of the nasopharyngeal microbiome to prevent infections caused by S. pneumoniae.

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“…In conclusion, as 1) dehydroxynocardamine is not a known virulence factor, 2), C. propinquum is considered a normal part of the nasal microbiota (33, 96, 97), and 3) expression of dnoA-G was detected in vivo ( Fig. 5 ), we propose that C. propinquum produces dehydroxynocardamine as a means to mediate exploitation competition for iron with other bacteria within the human nasal cavity.…”

Section: Discussionmentioning

confidence: 81%

Bacterial competition mediated by siderophore production among the human nasal microbiota

Stubbendieck

May

Chevrette

et al. 2018

Preprint

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19Resources available in the human nasal cavity are limited. Therefore, to successfully 20 colonize the nasal cavity, bacteria must compete for scarce nutrients. Competition may 21 occur directly through interference (e.g., antibiotics) or indirectly by nutrient 22 sequestration. To investigate the nature of nasal bacterial competition, we performed 23 co-culture inhibition assays between nasal Actinobacteria and Staphylococcus spp. We 24 found that Staphylococcus epidermidis isolates were sensitive to growth inhibition by 25 Actinobacteria but Staphylococcus aureus isolates were resistant to inhibition. Among 26 Actinobacteria, we observed that Corynebacterium spp. were variable in their ability to 27 inhibit S. epidermidis. We sequenced the genomes of ten Corynebacterium spp. 28 isolates, including three Corynebacterium propinquum that strongly inhibited S. 29 epidermidis and seven other Corynebacterium spp. isolates that only weakly inhibited S. 30 epidermidis. Using a comparative genomics approach, we found that the C. propinquum 31 genomes were enriched in genes for iron acquisition and encoded a biosynthetic gene 32 cluster (BGC) for siderophore production, absent in the non-inhibitory Corynebacterium 33 spp. genomes. Using a chromeazurol S assay, we confirmed that C. propinquum 34 produced siderophores. We demonstrated that iron supplementation rescued S. 35 epidermidis from inhibition by C. propinquum, suggesting that inhibition was due to iron 36 restriction through siderophore production. Using comparative metabolomics, we 37 identified the siderophore produced by C. propinquum as dehydroxynocardamine. 38

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Corynebacterium species nasopharyngeal carriage in asymptomatic individuals aged ≥ 65 years in Germany

Abstract: These results suggest that carriage of potentially toxigenic corynebacteria is very rare among people aged 65 and older in Germany. However, the low prevalence of protective anti-diphtheria toxin antibodies might pose a risk for acquiring diphtheria especially for the elderly.

Cited by 10 publications

References 21 publications

Asymptomatic carriage of Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, Group A Streptococcus and Staphylococcus aureus among adults aged 65 years and older

Asymptomatic carriage of Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, Group A Streptococcus and Staphylococcus aureus among adults aged 65 years and older

Non-diphtheriae Corynebacterium species are associated with decreased risk of pneumococcal colonization during infancy

Bacterial competition mediated by siderophore production among the human nasal microbiota

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