Corynebacterium fournierii sp. nov., isolated from the female genital tract of a patient with bacterial vaginosis

Abstract

Strain Marseille-P2948, a novel Gram-positive, catalase-positive bacterium was isolated from a vaginal sample of a patient with bacterial vaginosis. It was characterised using the taxonogenomic approach. Phylogenetic analysis revealed that the 16S rRNA and the rpoB genes exhibit 98.7 and 93.4% similarity, respectively, with those of Corynebacterium ureicelerivorans strain IMMIB RIV-301. Biochemical tests of strain Marseille-P2948 gave results that were similar to those of other validly named Corynebacterium species, whereas chemotaxonomic tests showed the presence of C, C, C and C in the fatty acid profile. The draft genome of strain Marseille-P2948 is 2,383,644 bp long in size with a G+C content of 65.03%. Of the 2210 predicted genes, 2147 are protein-coding genes and 63 are RNAs. Based on phenotypic, phylogenic and genomic results, it was concluded that the isolate represents a new species within the genus Corynebacterium. The name Corynebacterium fournierii sp. nov. is proposed and the type strain is Marseille-P2948 (= CSUR P2948 = DSM 103271).

Corynebacterium urinapleomorphum sp. nov., a new bacterial species isolated from human urine sample

“…To explore the diversity of human intestinal bacteria, the culturomics approach, based on diversified culture conditions, was designed to isolate species never cultivated before and also to complete the metagenomics of 16S rRNAs [2], [3], [4]. This culturomics approach has also been extended for the characterization of other human bacterial systems such as those of the vaginal and urinary tract [5], [6]. Recently, a new taxonomic method called taxonogenomics has been developed for a description associating the analysis of complete sequences of the genome and the phenotypic characteristics of new bacterial species [7].…”

Corynebacterium zhongnanshanii sp. nov. isolated from trachea of Marmota himalayana, Corynebacterium lujinxingii sp. nov. and Corynebacterium wankanglinii sp. nov. from human faeces

“…coyleae DSM 44184 T and C. jeddahense JCB T ); and acid production from d -glucose together with absence of esterase lipase (C8) activity make zg-913 T /zg-915 distinguishable from their five nearest relatives [35–37].…”

“…falsenii DSM 44353 T and C. fournieri Marseille-P2948 T ; conversely, their positive lipase (C14) activity was contrary to zg-320 T /zg-336) [36]; either absence of valine arylamidase activity or inability of producing acid from mannose differentiate zg-917 T /zg-910 from their nearest neighbours ( C. coyleae DSM 44184 T and C.…”

Characterisation of the Vaginal Microbiota Using Culturomics and Metagenomics Suggests Transplantation of Gut Microbiota Into the Vagina During Bacterial Vaginosis

“…According to culturomics analysis, β-diversity was higher in the bacterial vaginosis group [115/241 (48%)] than in the group with normal vaginal ora [110/261 (42%)]. Among the patients with bacterial vaginosis, 43% of isolated species (103/241) were not previously known to be present in the human vagina [27], including 14 new bacterial species and four new genera ( Table 2 [28][29][30][31][32][33][34][35][36][37][38][39][40]).…”