Corynebacterium argentoratense sp. nov., from the Human Throat

Abstract

A new Corynebacterium species, Corynebacteriurn argentoratense was isolated from the throats of four human patients. It is characterized by the presence of chemotype IV, a cell wall, corynomycolic acids, and a G+C content ranging from 60 to 61 mol%. Strains belonging to this species exhibit high levels of DNA relatedness as determined by DNA-DNA hybridization experiments (S1 nuclease procedure) but no close DNA relatedness with related Corynebacterium species. Phylogenies based on comparative analyses of nearly complete smallsubunit rDNA sequences confirmed the inclusion of this new species within the genus Corynebacteriurn and grouped it in a cluster with C. diphtheriae, C. ulcerans, C. pseudotubercubsis, and C. kutscheri. PCR experiments revealed an absence of the gene coding for diphtheria toxin. This new species can be identified by its mycolic acid pattern, fermentation of sugars, and enzymatic activities. Strain IBS B10697 (CIP 104296) is the type strain of C. argentorateme.

Phylogenetic Positions and Assignment of Swine and Ovine Corynebacterial Isolates Based on the 16S rDNA Sequence

“…Recently, a number of phylogenetic studies based on the 16S rDNA sequence established new criteria for the intrageneric taxonomy of the genus Corynebacterium and relationships among the closely related mycolic acid-containing genera such as Dietzia, Gordona, Mycobacterium, Nocardia, Rhodococcus, Tsukamurella and Turicella (11,(16)(17)(18)(19)(20)(21)24). By these studies, the determination of 16S rDNA sequences of the species of genus Corynebacterium has virtually been completed, and the phylogenetic relationships have also been deduced from the data.…”

Corynebacterium testudinoris sp. nov., from a tortoise, and Corynebacterium felinum sp. nov., from a Scottish wild cat.

“…ndez-Garayza! bal et al, 1997Funke et al, 1997Funke et al, , 1998Riegel et al, 1995). Most of these novel species have come to light primarily as the result of the availability of more precise molecular diagnostic tools (e.g.…”

Corynebacterium sundsvallense sp. nov., from human clinical specimens

“…The GenBank accession number for the 165 rRNA gene sequence of strain CCUG 36622l is YO9655 toratense (Riegel et al, 1995a), Corynebacterium afermentans (Riegel et al, 1993a), Corynebacterium auris (Funke et al, 1995b), Corynebacterium coyleae (Funke et al, 1997c), Corynebacterium durum (Riegel et al, 1997a), Corynebacterium glucuronolyticum (Funke et al, 1995a), Corynebacterium lipophilojlavurn (Funke et al, 1997a), Corynebacterium macginleyi (Riegel et al, 1995b), Corynebacterium mucqaciens (Funke et al, 1997b), Corynebacterium propinquum (Riegel et al, 1993b), Corynebacterium singulare (Riegel et al, 1997b) and Corynebacterium riegelii (Funke et al, 1998). In this article we report the polyphasic taxonomic characterization of three coryneform-like isolates from human clinical specimens.…”