Corticosterone is required for the prolactin receptor gene expression in the late pregnant mouse mammary gland

Mizoguchi, Yasushi; Yamaguchi, Hirohito; Aoki, Fugaku; Enami, Jumpei; Sakai, Senkiti

doi:10.1016/s0303-7207(97)00134-2

Cited by 45 publications

(28 citation statements)

References 38 publications

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“…These results further illustrates the signiWcant role PRL plays in regulating lipid biosynthesis through the upregulation of PRLR mRNA paralleled with a comparable increase in lipogenic genes in the mammary gland (Barber et al 1997;Neville and Picciano 1997;Ros et al 1990;Vernon et al 1999). However, Prlr-l mRNA is partially upregulated by glucocorticoids (GC) (Mizoguchi et al 1997;Phillips et al 1997Phillips et al , 1999Sakai et al 1994).…”

Section: Introductionmentioning

confidence: 66%

“…Our recent study revealed that the aberrant PRL proWle during the peripartum period in HG-exposed dams was accompanied by a decrease in Prlr mRNA abundance (Patel et al 2007). In view of the documented eVects of lactogenic hormones on induction of PRLR expression (Holcomb et al 1976;McNeilly and Friesen 1977;Mizoguchi et al 1997;Phillips et al 1997Phillips et al , 1999Sakai et al 1994) and reported downregulation of PRLR transcripts in HG-exposed animals, we hypothesized that supplementation with either PRL or GC would correct the suppressed levels of PRLR transcripts and abrogate the HG-induced eVect(s) on lipogenesis and result in increased neonatal survival. Therefore, the objective of this study was to determine if PRL or GC supplementation would prevent or attenuate the HG eVects on periparturient dam metabolism.…”

Section: Introductionmentioning

confidence: 99%

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Lipogenesis impaired in periparturient rats exposed to altered gravity is independent of prolactin and glucocorticoid secretion

et al. 2008

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Perturbed prolactin (PRL) secretion and concomitant downregulation of PRL receptor (PRLR) in periparturient dams exposed to altered gravity are linked to aberrant lipogenesis and reduced neonatal survival. PRL and glucocorticoids (GC) are known to modulate PRLR expression. We hypothesized that improving levels of PRLR would mitigate the increased gravity [hypergravity (HG)]-induced effects of impaired mammary lipogenesis and increase neonatal survival. The objective of this study was to determine if prepartum PRL or GC supplementation would override the HG-induced repression of PRLR along with lipogenic genes and increase tissue fatty acid synthesis. Pregnant rats were exposed to either 2g (HG) or kept at 1g (control) from day 11 of gestation (G11) through Postnatal day 1 (P1). HG exposed rats were supplemented with either PRL or corticosterone or a placebo from G13 to P1. On P1, mammary, liver and adipose tissues were collected to measure glucose incorporation into lipids and mRNA abundance of PRL long and short form receptors (Prlr-l, Prlr-s), glucocorticoid receptor (Nr3c1), Acetyl CoA carboxylase-alpha (Acaca), fatty acid synthase (Fasn), lipoprotein lipase (Lpl), Sterol Regulatory Element Binding Protein-1 (Srebp1) and protein kinase B (Akt1) genes by quantitative polymerase chain reaction (qPCR). PRL and GC supplementation had a limited effect on lipogenesis in the three tissues of HG group likely due to their inability to increase abundance of key down-regulated genes, including Prlr-l and Nr3c1. There was no difference in the abundance of genes coding for milk proteins or those associated with milk fat globule formation and secretion. These data suggest that reduced lipogenesis in HG exposed dams is independent of PRL and GC secretion but may be associated with dysregulation of multiple metabolic regulators at the level of mRNA expression.

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Section: Introductionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Lipogenesis impaired in periparturient rats exposed to altered gravity is independent of prolactin and glucocorticoid secretion

et al. 2008

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“…For instance in rats, prolactin concentration in plasma increases significantly just before parturition and remains high for the entire lactation [110], accompanied also by a concomitant increase in prolactin receptor [111] which appears similar to the pattern of prolactin receptor in mouse mammary during lactation [112]. In contrast, prolactin concentration in dairy cows increases dramatically at the onset of lactation and decreases afterwards to levels observed prior parturition, and prolactin receptor expression does not increase [4].…”

Section: Regulation Of Milk Protein Expressionmentioning

confidence: 74%

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Bionaz¹,

Hurley²,

Loor³

2012

Milk Protein

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“…We show here that the LAO gene is also expressed upon the onset of milk synthesis. In the circulating plasma, progesterone decreases and corticosterone increases at the end of pregnancy as well as after ovariectomy in mice (7). Glucocorticoid (cortisol or corticosterone) is essential to activate the PRL receptor gene (7) and to maintain the number of PRL receptors (23).…”

Section: L-amino Acid Oxidase Of Mouse Milkmentioning

confidence: 99%

“…It is well established that prolactin (PRL) 1 is a key hormone to regulate lactose and casein syntheses. PRL is able to stimulate the mammary gland at the end of pregnancy since the PRL receptor gene is expressed half a day before parturition (7). To find the genes expressed highly in the late pregnant mouse mammary gland, mRNA screening was carried out using differential display methods.…”

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confidence: 99%

Characterization and Expression of l-Amino Acid Oxidase of Mouse Milk

Sun¹,

Nonobe²,

Kobayashi³

et al. 2002

Journal of Biological Chemistry

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L-Amino acid oxidase (LAO) was purified from mouse milk. LAO reacted with L-amino acids in an apparent order of Phe > Met, Tyr > Cys, Leu > His > > other 11 amino acids tested and produced H 2 O 2 in a dose-and time-dependent manner. LAO in milk had a molecular mass of about 113 kDa and was converted to a 60-kDa protein by SDS-PAGE. LAO consisted of two subunits. The N-and C-terminal amino acid sequence determination followed by cDNA cloning showed that the 60-kDa protein consisted of 497 amino acids. LAO mRNA spanned about 2.0 kb, and its expression was found only in the mammary epithelial cells. Glucocorticoid was essential for LAO gene expression. Thus, the LAO gene is expressed acutely upon the onset of milk synthesis. LAO mRNA increased 1 day before parturition, peaked during early to mid-lactation, and decreased at the end of lactation. This is the first demonstration showing that LAO is present in milk. Mastitis is caused by an intramammary bacterial infection. As mouse milk produced H 2 O 2 using endogenous free amino acids, we suggest that LAO, together with free amino acids, is responsible for killing bacteria in the mammary gland.

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Corticosterone is required for the prolactin receptor gene expression in the late pregnant mouse mammary gland

Cited by 45 publications

References 38 publications

Lipogenesis impaired in periparturient rats exposed to altered gravity is independent of prolactin and glucocorticoid secretion

Lipogenesis impaired in periparturient rats exposed to altered gravity is independent of prolactin and glucocorticoid secretion

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Characterization and Expression of l-Amino Acid Oxidase of Mouse Milk

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