Cortical bone elasticity measured by resonant ultrasound spectroscopy is not altered by defatting and synchrotron X-ray imaging

Cai, Xiran; Peralta, Laura; Giron, A.; Helfen, Lukas; Olivier, Cécile; Peyrin, Françoise; Laugier, Pascal; Grimal, Quentin

doi:10.1016/j.jmbbm.2017.05.012

Cited by 12 publications

(11 citation statements)

References 31 publications

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“…There was no bias and the mean and standard deviation of differences appeared to be constant throughout the range of Q −1 44 values. This analysis complements the analysis, conducted on the same specimens, of the effect of defatting and irradiation on elastic properties which was reported in [26].…”

Section: Appendix B Effect Of Defatting and X-ray Radiation On Viscoelastic Dampingsupporting

confidence: 77%

“…All specimens were frozen and stored at −20 • C between tests. They were then slowly thawed and immersed in 0.9% NaCl saline before testing to ensure full hydration [26]. The nominal size of the specimens #1 used for RUS was 3 × 4 × 5mm 3 in radial (x 1 ), circumferential (x 2 ) and axial (x 3 ) directions, respectively.…”

Section: Specimensmentioning

confidence: 99%

“…Specimens #1 used in RUS were kept hydrated prior to experiment and all RUS measurements were made on a fully hydrated specimen. After a first set of RUS measurements, specimens #1 were defatted following a protocol which prevents the risk of infections and allows the specimen conservation at room temperature [26].…”

Section: Specimensmentioning

confidence: 99%

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Cortical bone viscoelastic damping assessed with resonant ultrasound spectroscopy reflects porosity and mineral content

Fan

Cai

Follet

et al. 2021

Journal of the Mechanical Behavior of Biomedical Materials

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Viscoelasticity is an essential property of bone related to fragility, which is altered in aging and bone disease.Bone viscoelastic behavior is attributed to several mechanisms involving collagen and mineral properties, porosities, and bone hierarchical tissue organization. We aimed to assess the relationships between cortical bone viscoelastic damping measured with Resonant Ultrasound Spectroscopy (RUS), microstructural and compositional characteristics. We measured 52 bone specimens from the femur of 26 elderly human donors.RUS provided a shear damping coefficient at a frequency of the order of 150 kHz. The characteristics of the structure of the vascular pore network and tissue mineral density were measured using synchrotron radiation high-resolution computed tomography (SR-µCT). Fourier transformed infrared microspectroscopy (FTIRM) was used to quantify mineral-to-organic phase ratio, mineral maturity, crystallinity, and collagen maturity.Cross-links were quantified from biochemistry. Viscoelastic damping was found to increase with vascular porosity (r = 0.68), to decrease with the degree of mineralization of the extravascular matrix (r = −0.68), and was marginally affected by collagen. We built a multilinear model suggesting that when porosity is controlled, the variation of mineral content explains a small additional part of the variability of damping. The work supports the consideration of viscoelasticity measurement as a potential biomarker of fragility and provides a documentation of bone viscoelastic behavior and its determinants in a frequency range rarely investigated.

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Section: Appendix B Effect Of Defatting and X-ray Radiation On Viscoelastic Dampingsupporting

confidence: 77%

Section: Specimensmentioning

confidence: 99%

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Cortical bone viscoelastic damping assessed with resonant ultrasound spectroscopy reflects porosity and mineral content

Fan

Cai

Follet

et al. 2021

Journal of the Mechanical Behavior of Biomedical Materials

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“…Following indentation, the samples were removed from the sample holder and cleaned in the ultrasonic bath filled with soapy water for 15 min. Subsequently, they were defatted in a 1:1 methanol -diethylether solution for one day 31 . They were air-dried before being placed, dry and defatted, in the cone beam micro-CT (EasyTom XL Micro, RX Solutions, Chavanod, France).…”

Section: Methodsmentioning

confidence: 99%

Tibial subchondral trabecular bone micromechanical and microarchitectural properties are affected by alignment and osteoarthritis stage

Renault

Carmona

Tzioupis

et al. 2020

Sci Rep

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At advanced knee osteoarthritis (OA) stages subchondral trabecular bone (STB) is altered. Lower limb alignment plays a role in OA progression and modify the macroscopic loading of the medial and lateral condyles of the tibial plateau. How the properties of the STB relate to alignment and OA stage is not well defined. OA stage (KL scores 2-4) and alignment (HKA from 17° Varus to 8° Valgus) of 30 patients were measured and their tibial plateau were collected after total knee arthroplasty. STB tissue elastic modulus, bone volume fraction (BV/TV) and trabecula thickness (Tb.Th) were evaluated with nanoindentation and µCT scans (8.1 µm voxel-size) of medial and lateral samples of each plateau. HKA and KL scores were statistically significantly associated with STB elastic modulus, BV/TV and Tb.Th. Medial to lateral BV/TV ratio correlated with HKA angle (R = −0.53, p = 0.016), revealing a higher ratio for varus than valgus subjects. STB properties showed lower values for KL stage 4 patients. Tissue elastic modulus ratios and BV.TV ratios were strongly correlated (R = 0.81, p < 0.001). Results showed that both micromechanical and microarchitectural properties of STB are affected by macroscopic loading at late stage knee OA. For the first time, a strong association between tissue stiffness and quantity of OA STB was demonstrated.

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“…Eight samples were taken from deceased female donors (between 56 and 95 years old) provided by the Centre du Don des Corps (University Paris 5) and the Department of Anatomy Rockefeller, University Lyon. Except the sex and the age, no other information regarding disease status or medication history was available in accordance with legal clauses stated in the French Code of Public Health Ethics 34 . Femoral diaphyses were extracted from different parts of these cadaveric subjects and wrapped in gauze soaked with saline to keep hydrated, and stored at ‒20°C until sample preparation.…”

Section: Methodsmentioning

confidence: 99%

Quantification of the bone lacunocanalicular network from 3D X‐ray phase nanotomography images

Pacureanu

Olivier

et al. 2020

Journal of Microscopy

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Summary There is a growing interest in developing 3D microscopy for the exploration of thick biological tissues. Recently, 3D X‐ray nanocomputerised tomography has proven to be a suitable technique for imaging the bone lacunocanalicular network. This interconnected structure is hosting the osteocytes which play a major role in maintaining bone quality through remodelling processes. 3D images have the potential to reveal the architecture of cellular networks, but their quantitative analysis remains a challenge due to the density and complexity of nanometre sized structures and the need to handle and process large datasets, for example, 20483 voxels corresponding to 32 GB per individual image in our case. In this work, we propose an efficient image processing approach for the segmentation of the network and the extraction of characteristic parameters describing the 3D structure. These parameters include the density of lacunae, the porosity of lacunae and canaliculi, and morphological features of lacunae (volume, surface area, lengths, anisotropy etc.). We also introduce additional parameters describing the local environment of each lacuna and its canaliculi. The method is applied to analyse eight human femoral cortical bone samples imaged by magnified X‐ray phase nanotomography with a voxel size of 120 nm, which was found to be a good compromise to resolve canaliculi while keeping a sufficiently large field of view of 246 μm in 3D. The analysis was performed on a total of 2077 lacunae showing an average length, width and depth of 17.1 μm × 9.2 μm × 4.4 μm, with an average number of 58.2 canaliculi per lacuna and a total lacuno‐canalicular porosity of 1.12%. The reported descriptive parameters provide information on the 3D organisation of the lacuno‐canalicular network in human bones.

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Cortical bone elasticity measured by resonant ultrasound spectroscopy is not altered by defatting and synchrotron X-ray imaging

Cited by 12 publications

References 31 publications

Cortical bone viscoelastic damping assessed with resonant ultrasound spectroscopy reflects porosity and mineral content

Cortical bone viscoelastic damping assessed with resonant ultrasound spectroscopy reflects porosity and mineral content

Tibial subchondral trabecular bone micromechanical and microarchitectural properties are affected by alignment and osteoarthritis stage

Quantification of the bone lacunocanalicular network from 3D X‐ray phase nanotomography images

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