Cortical and Subcortical Changes Preceding Furrow Formation in the Cleavage of Newt Eggs

Sawai, Tsuyoshi; Kubota, Tomoyuki; Kojima, M.

doi:10.1111/j.1440-169x.1969.00246.x

Cited by 17 publications

(4 citation statements)

References 4 publications

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“…In the cleavage of amphibian eggs, the position of the cleavage plane is determined by the location of the FIC under the cortex (15,17,18), and the distribution of the FIC is determined by microtubules of the MA, as judged from the relationship between the position of the MA and the cleavage plane (8,22,23,30). Thus, the inhibition of furrow formation in the Exp.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, we have observed that the subcortical cytoplasm along the cleavage plane induces a furrow-like dent on the cortex when transplanted beneath the cortex in a region outside the cleavage plane (15,17,18), indicating that the subcortical cytoplasm along the cleavage plane contains some factor involved in furrow formation (furrow-inducing cytoplasm; FIC). Further, I have shown that the cortex acquires ability to form a furrow in response to the inductive activity of the FIC during cleavage (1 5, 17).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Microtubular Poisons on Cleavage Furrow Formation and Induction of Furrow‐like Dent in Amphibian Eggs

Sawai¹

1992

Dev Growth Differ

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The effects of the microtubular poisons colchicine, vinblastine and nocodazole, on cleavage furrow formation and induction of furrow-like dents in eggs of the newt, Cynops pyrrhogaster, were examined.Solutions of the poisons were injected beneath the cortex around the small initial furrow, or around the advancing tip of the furrow of eggs during the first cleavage. This resulted in prompt block of the progress of the furrow at the injection site, and subsequent total regression of the furrow or incomplete cleavage. The ability of the cortex of a cleavage-arrested blastomere to form a furrow-like dent was tested by inhibiting furrow formation of one blastomere of two-cell embryos by injection of the microtubular poisons, and then transplantation of the blastomere under the cortex of the animal half with furrow-inducing cytoplasm (FIC) taken from normally cleaving eggs. No dent was formed. Moreover, FIC from eggs treated with a poison had no ability to induce a dent on the surface of normally cleaving eggs These results show that microtubule structures are directly involved in formation of a cleavage furrow

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Microtubular Poisons on Cleavage Furrow Formation and Induction of Furrow‐like Dent in Amphibian Eggs

Sawai¹

1992

Dev Growth Differ

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“…We have demonstrated that subcortical cytoplasm on a cleavage plane of amphibian eggs induces furrow-like dents when transplanted to a subcortical region outside the plane (Sawai, 1972(Sawai, , 1983Sawai et al, 1969). This indicates that subcortical cytoplasm on a cleavage plane contains a factor responsible for furrow formation, i.e.…”

Section: Introductionmentioning

confidence: 78%

“…We have shown by the FIC transplantation experiments (Sawai, 1972(Sawai, , 1983Sawai et al, 1969) that a furrow was established by an interaction of a cortical and a cytoplasmic factor . With regard to the relationship between the role of microtubule structures and that of the two factors in cortical events, several reports had indicated that microtubular poisons have no effect on the emergence of cortical changes such as travelling of a contractile wave, rounding-up of eggs or increase of stiffness (Hara et al, 1980;Sakai and Kubota, 1981;Sakai and Shinagawa, 1983;Shinagawa, 1983;Yoneda et al, 1982), which are regarded as a reflection of a cortical change indispensable for furrow formation (Sawai, 1979;Sawai and Yoneda, 1974).…”

Section: Discussionmentioning

confidence: 99%

Evidences for Direct Involvement of Microtubules in Cleavage Furrow Formation in Newt Eggs

Sawai

1998

Zoological Science

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This paper aims at examining the effect of colchicine, a microtubular poison, on the process of furrow formation in whole eggs and egg fragments as well as the process of artificial induction of furrow-like dents, in eggs of the newt, Cynops pyrrhogaster. To apply colchicine locally to eggs, the eggs were slit across or along a furrow in a colchicine solution during first cleavage. When a slit was made across or in front of a growing furrow at the onset of its growth, the furrow quickly ceased growing and often regressed. Cortices containing an entire growing furrow were isolated along with a thin layer of subcortical cytoplasm immediately after the start of the first cleavage. Furrows in the cortices degenerated when the cortices were cultured in a colchicine solution, whereas they continued growing when they were cultured in Holtfreter's saline. Furrow-inducing cytoplasm was injected to a site beneath the cortex in the animal half of the egg during first cleavage. When a small slit was made close to the site of the injection in a colchicine solution, no furrow-like dent was induced. These results imply that microtubules are directly involved in the generation and growth of cleavage furrows.

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Analysis of cleavage stimulus by means of micromanipulation of sea urchin eggs

Hiramoto¹

1971

Experimental Cell Research

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Cortical and Subcortical Changes Preceding Furrow Formation in the Cleavage of Newt Eggs

Abstract: In the first cleavage of the egg of the newt, Cynops(Triturus)pyrrhogaster,

Cited by 17 publications

References 4 publications

Effect of Microtubular Poisons on Cleavage Furrow Formation and Induction of Furrow‐like Dent in Amphibian Eggs

Effect of Microtubular Poisons on Cleavage Furrow Formation and Induction of Furrow‐like Dent in Amphibian Eggs

Evidences for Direct Involvement of Microtubules in Cleavage Furrow Formation in Newt Eggs

Analysis of cleavage stimulus by means of micromanipulation of sea urchin eggs

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