Evidences for Direct Involvement of Microtubules in Cleavage Furrow Formation in Newt Eggs

Sawai, Tsuyoshi

doi:10.2108/zsj.15.51

Cited by 2 publications

(2 citation statements)

References 38 publications

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“…Growing evidence from these studies indicates that in these cells interpolar microtubules or factors associated with them play more important roles in inducing cleavage furrows than astral microtubules (Wheatley and Wang, 1996;Oegema et al, 1997;Wheatley et al, 1998). Experiments using newt egg cells (Sawai, 1998) and nematode embryos (Raich et al, 1998) also suggest a continual requirement of interpolar microtubules for the furrow contraction.…”

Section: What Determines the Position Of The Cleavage Furrow?mentioning

confidence: 99%

Myosin II-Independent Cytokinesis in Dictyostelium. Its Mechanism and Implications.

Uyeda¹,

Kitayama²,

Yumura

2000

Cell Struct. Funct.

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ABSTRACT. Similar to higher animal cells, ameba cells of the cellular slime mold Dictyostelium discoideum form contractile rings containing filaments of myosin II during mitosis, and it is generally believed that contraction of these rings bisects the cells both on substrates and in suspension. In suspension, mutant cells lacking the single myosin II heavy chain gene cannot carry out cytokinesis, become large and multinucleate, and eventually lyze, supporting the idea that myosin II plays critical roles in cytokinesis. These mutant cells are however viable on substrates. Detailed analyses of these mutant cells on substrates revealed that, in addition to "classic" cytokinesis which depends on myosin II ("cytokinesis A"), Dictyostelium has two distinct, novel methods of cytokinesis, 1) attachment-assisted mitotic cleavage employed by myosin II null cells on substrates ("cytokinesis B"), and 2) cytofission, a cell cycle-independent division of adherent cells ("cytokinesis C"). Cytokinesis A, B, and C lose their function and demand fewer protein factors in this order. Cytokinesis B is of particular importance for future studies. Similar to cytokinesis A, cytokinesis B involves formation of a cleavage furrow in the equatorial region, and it may be a primitive but basic mechanism of efficiently bisecting a cell in a cell cycle-coupled manner. Analysis of large, multinucleate myosin II null cells suggested that interactions between astral microtubules and cortices positively induce polar protrusive activities in telophase. A model is proposed to explain how such polar activities drive cytokinesis B, and how cytokinesis B is coordinated with cytokinesis A in wild type cells.

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Section: What Determines the Position Of The Cleavage Furrow?mentioning

confidence: 99%

Myosin II-Independent Cytokinesis in Dictyostelium. Its Mechanism and Implications.

Uyeda¹,

Kitayama²,

Yumura

2000

Cell Struct. Funct.

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“…Furrow formation stimulated by treatment of newt eggs with furrow-inducing agents was ablated when colchicine was applied locally to the furrow [80]. Furthermore, local colchicine application shortly following furrow constriction resulted in arrest and, sometimes, retraction of the furrow.…”

Section: Interplay Between Microtubule and Actin Network During Cytomentioning

confidence: 99%

Regulation of cytokinesis

Wolf¹,

Chew²,

Chisholm³

1999

CMLS, Cell. Mol. Life Sci.

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At the end of mitosis, daughter cells are separated from each other by cytokinesis. This process involves equal partitioning and segregation of cytoplasm between the two cells. Despite years of study, the mechanism driving cytokinesis in animal cells is not fully understood. Actin and myosin are major components of the contractile ring, the structure at the equator between the dividing cells that provides the force necessary to constrict the cytoplasm. Despite this, there are also tantalizing results suggesting that cytokinesis can occur in the absence of myosin. It is unclear what the roles are of the few other contractile ring components identified to date. While it has been difficult to identify important proteins involved in cytokinesis, it has been even more challenging to pinpoint the regulatory mechanisms that govern this vital process. Cytokinesis must be precisely controlled both spatially and temporally; potential regulators of these parameters are just beginning to be identified. This review discusses the recent progress in our understanding of cytokinesis in animal cells and the mechanisms that may regulate it.

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Evidences for Direct Involvement of Microtubules in Cleavage Furrow Formation in Newt Eggs

Cited by 2 publications

References 38 publications

Myosin II-Independent Cytokinesis in Dictyostelium. Its Mechanism and Implications.

Myosin II-Independent Cytokinesis in Dictyostelium. Its Mechanism and Implications.

Regulation of cytokinesis

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