“…In recent years, the interest for this approach has risen again ( Costa et al, 2018 ; Altieri et al, 2019 ; Grzegorczyk et al, 2019 ; Dziedzic et al, 2023 ). The combination of classical, neuroanatomical, fiber-dissection techniques and more advanced neuroradiological approaches such as diffusion tensor imaging and fiber tractography, have broadened the understanding of several white-matter connections ( Holl et al, 2011 ; Akakin et al, 2014 ; Sarubbo et al, 2015 ; Meola et al, 2016 ; Burks et al, 2017 ; Goryainov et al, 2017 ; Pascalau et al, 2018 ; Flores-Justa et al, 2019 ; Shah et al, 2019 ; Dziedzic et al, 2021a , 2022 ; Rauch et al, 2021 ), even leading to the discovery of novel structures ( Shinohara et al, 2020 ). Throughout the last 150 years, a plethora of fixation and staining methods has been described.…”