Corrosion Cast Demonstration of Retinal Vasculature of Normal Wistar-Kyoto Rats

Bhutto, Imran Ahmed; Amemiya, Tsugio

doi:10.1159/000147730

Cited by 33 publications

(27 citation statements)

References 4 publications

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“…Occasionally, there was a leakage of plastic material or incomplete filling of retinal capillaries, perhaps due to differences in physiological pressure between vessels and capillaries. 16 In the present study, a microaneurysm-like feature was taken. It was uncertain whether this feature was due to a leakage of resin.…”

Section: Discussionmentioning

confidence: 99%

Retinal Changes in Otsuka Long-Evans Tokushima Fatty Rats (Spontaneously Diabetic Rat)—Possibility of a New Experimental Model for Diabetic Retinopathy

Lu¹

2003

Japanese Journal of Ophthalmology

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Section: Discussionmentioning

confidence: 99%

Retinal Changes in Otsuka Long-Evans Tokushima Fatty Rats (Spontaneously Diabetic Rat)—Possibility of a New Experimental Model for Diabetic Retinopathy

Lu¹

2003

Japanese Journal of Ophthalmology

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“…The vascular casts were prepared by our previously described technique (Bhutto and Amemiya, 1995a). In brief, both common carotid arteries were cannulated and the jugular veins were then cut.…”

Section: Corrosion Casts and Scanning Electron Microscopymentioning

confidence: 99%

Microvascular architecture of the rat choroid: Corrosion cast study

Bhutto

Amemiya

2001

Anat. Rec.

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This study presents the details of the microvasculature of the rat choroid visualized by scanning electron microscopy of vascular corrosion casts. Wistar Kyoto rats were anesthetized with intraperitoneal sodium pentobarbital. The vascular system was perfused with heparinized saline, and Mercox resin was injected into the cannulated carotid arteries. After polymerization of the resin, the vascular casts were macerated with potassium hydroxide, washed with water, and freeze dried. The casts were examined with a Hitachi S-2360N scanning electron microscope (SEM). Corrosion casts of the entire choroidal vasculature revealed that the two long posterior ciliary arteries supplied the entire uveal vasculature. In the posterior choroid, these arteries formed five to seven branches on each side supplying the adjacent choriocapillaris. No interarterial or arteriovenous anastomoses were seen. The choriocapillaris appeared as a nonhomogeneous and nonlobular monolayer capillary network, consisting of dense honeycomb and irregular patterns. There are two distinct venous systems in the rat choroid. The venous blood from the central region, peripapillary choroid, and sometimes the optic nerve head drain into the posterior ciliary vein. The venous blood from the iris, ciliary body, anterior choroid, and half of the posterior choroid drain into the vortex veins. Corrosion casts and the SEM have shown details of the choroidal vascular architecture. These three-dimensional observations indicate that the rat choroidal vasculature has different features from those of humans and other primates. Despite these interspecies differences, the establishment of a thorough baseline concept of choroidal vasculature should permit additional studies of the choroidal pathology and enable the proper interpretation of results from rat experimental models for extrapolation to humans. Anat Rec 264: [63][64][65][66][67][68][69][70][71] 2001.

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“…Vascular casts were prepared according to our previously described technique [13]. Briefly, the rats were anesthetized with intraperitoneal sodium pentobarbital.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Vascular Architecture of Degenerated Retina in WBN/Kob Rats: Corrosion Cast and Electron Microscopic Study

1999

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The purpose of the present study is to determine the changes of vascular architecture in the degenerated retina. We used mainly corrosion casts of the retinal vasculature and scanning electron microscopy to obtain a wide three-dimensional view. WBN/Kob rats (a spontaneously diabetic strain) were used because their outer retinas degenerate and become very thin with age. In 15-month-old rats, localized constriction and irregular caliber of the capillaries were evident in the vascular casts. Two layers of capillary network in the retina were maintained, but the capillaries were decreased in number. Numerous loop formations were present in the superficial capillary networks. Neither microaneurysms nor arteriovenous shunts were seen in young and old rats. Transmission electron microscopy revealed that capillary pericytes in the inner and outer plexiform layers had thickened basement membranes and that endothelial cells had many vesicles in their cytoplasm. Thus the retinal capillary changes in WBN/Kob rats are nondiabetic but due to hereditary retinal degeneration, although the systemic and pancreatic abnormalities in this rat strain are diabetic. Even when the retina becomes very thin, two layer capillary networks remain.

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Corrosion Cast Demonstration of Retinal Vasculature of Normal Wistar-Kyoto Rats

Cited by 33 publications

References 4 publications

Retinal Changes in Otsuka Long-Evans Tokushima Fatty Rats (Spontaneously Diabetic Rat)—Possibility of a New Experimental Model for Diabetic Retinopathy

Retinal Changes in Otsuka Long-Evans Tokushima Fatty Rats (Spontaneously Diabetic Rat)—Possibility of a New Experimental Model for Diabetic Retinopathy

Microvascular architecture of the rat choroid: Corrosion cast study

Vascular Architecture of Degenerated Retina in WBN/Kob Rats: Corrosion Cast and Electron Microscopic Study

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