Corrigendum to “Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis” [Metab. Eng. 13 (2011) 656–665]

Young, Jamey D.; Shastri, Avantika A.; Stephanopoulos, Gregory; Morgan, John A.

doi:10.1016/j.ymben.2012.01.005

Cited by 11 publications

(10 citation statements)

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“…This finding suggests that the amount of EFE enzyme is rate-limiting when only one copy (and possibly even two copies) of Sy-efe is expressed. Importantly, the maximum percentage ($5.5%) of fixed carbon diverted to ethylene production surpassed the published carbon partition rate into the TCA cycle, 14 which suggests that there may be significant alterations to the carbon flux in our ethylene forming strains.…”

Section: Carbon Partitioningmentioning

confidence: 90%

Sustained photosynthetic conversion of CO2 to ethylene in recombinant cyanobacterium Synechocystis 6803

Ungerer

Tao

Davis

et al. 2012

Energy Environ. Sci.

221

201

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Section: Carbon Partitioningmentioning

confidence: 90%

Sustained photosynthetic conversion of CO2 to ethylene in recombinant cyanobacterium Synechocystis 6803

Ungerer

Tao

Davis

et al. 2012

Energy Environ. Sci.

221

201

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“…Metabolic flux analysis has shown that as much as 25% of all inorganic carbon fixation occurs via PepC in Synechocystis cultured under mixotrophic or heterotrophic conditions [60]. An additional protein, malic enzyme (ME), catalyses the reversible conversion of malate, a TCA intermediate, and pyruvate [61]. Deletion of ME in Synechocystis results in a mutant that displays poor growth when exposed to continuous but not diurnal light [62].…”

Section: Alternate Biosynthetic Pathways Linking Metabolites Of the Tmentioning

confidence: 99%

Current knowledge and recent advances in understanding metabolism of the model cyanobacteriumSynechocystissp. PCC 6803

2020

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Cyanobacteria are key organisms in the global ecosystem, useful models for studying metabolic and physiological processes conserved in photosynthetic organisms, and potential renewable platforms for production of chemicals. Characterizing cyanobacterial metabolism and physiology is key to understanding their role in the environment and unlocking their potential for biotechnology applications. Many aspects of cyanobacterial biology differ from heterotrophic bacteria. For example, most cyanobacteria incorporate a series of internal thylakoid membranes where both oxygenic photosynthesis and respiration occur, while CO2 fixation takes place in specialized compartments termed carboxysomes. In this review, we provide a comprehensive summary of our knowledge on cyanobacterial physiology and the pathways in Synechocystis sp. PCC 6803 (Synechocystis) involved in biosynthesis of sugar-based metabolites, amino acids, nucleotides, lipids, cofactors, vitamins, isoprenoids, pigments and cell wall components, in addition to the proteins involved in metabolite transport. While some pathways are conserved between model cyanobacteria, such as Synechocystis, and model heterotrophic bacteria like Escherichia coli, many enzymes and/or pathways involved in the biosynthesis of key metabolites in cyanobacteria have not been completely characterized. These include pathways required for biosynthesis of chorismate and membrane lipids, nucleotides, several amino acids, vitamins and cofactors, and isoprenoids such as plastoquinone, carotenoids, and tocopherols. Moreover, our understanding of photorespiration, lipopolysaccharide assembly and transport, and degradation of lipids, sucrose, most vitamins and amino acids, and haem, is incomplete. We discuss tools that may aid our understanding of cyanobacterial metabolism, notably CyanoSource, a barcoded library of targeted Synechocystis mutants, which will significantly accelerate characterization of individual proteins.

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“…PCC 6803 under photoautotrophic growth conditions. 3,22 As one of the most iconic pathways in the central metabolism, the TCA cycle has a dual role in ATP synthesis and precursor metabolite (e.g. oxaloacetate, succinate and 2-OG) supply.…”

Section: Introductionmentioning

confidence: 99%

Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803

Zhu

Xie

et al. 2015

Green Chem.

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Ethylene is widely used in the petrochemical industry and has traditionally been produced via the steam cracking of petroleum-based feedstock. The exploration of sustainable and carbon-neutral methods of producing ethylene from the renewable feedstock seems promising. The direct photosynthetic production of ethylene after the recycling of carbon dioxide shows great potential. In this study, continuous and stable ethylene production was achieved in Synechocystis sp. PCC 6803 by introducing a codonoptimized ethylene-forming enzyme (EFE) from Pseudomonas syringae pv. sesami and using 2-oxoglutarate (2-OG) as the substrate. Based on diverse promoter screening, PcpcB was proved to be a highly efficient promoter for ethylene production in cyanobacteria. The genes encoding 2-OG decarboxylase (OGDC) and succinic semialdehyde dehydrogenase (SSADH) in the tricarboxylic acid (TCA) cycle in Synechocystis sp. PCC 6803 were identified, and the TCA cycle was genetically modified by blocking these two enzymes with the simultaneous overexpression of EFE. Meanwhile, a gene encoding 2-OG permease (KgtP) from E. coli was introduced into the phaAB loci to increase the 2-OG supply. A peak volumetric production rate of 9.7 mL L −1 h −1 for ethylene was eventually achieved in the Synechocystis recombinant (XX110), with the genetic modification of the TCA cycle and heterologous expression of 2-oxoglutarate permease by the modified semi-continuous cultivation.

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Corrigendum to “Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis” [Metab. Eng. 13 (2011) 656–665]

Cited by 11 publications

References 0 publications

Sustained photosynthetic conversion of CO2 to ethylene in recombinant cyanobacterium Synechocystis 6803

Sustained photosynthetic conversion of CO2 to ethylene in recombinant cyanobacterium Synechocystis 6803

Current knowledge and recent advances in understanding metabolism of the model cyanobacteriumSynechocystissp. PCC 6803

Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803

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