Corrigendum to “Characterization of polyphenol oxidase from Fennel (Foeniculum vulgare Mill.) seeds as a promising source” [Int. J. Biol. Macromol. 170 (2021) 261–271]

Karakuş, Yonca Yüzügüllü; Yildirim, Binnur; Acemi, Arda

doi:10.1016/j.ijbiomac.2021.05.084

Cited by 3 publications

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“…Conversely, a stronger inhibition effect was observed for urea at 5 mM with 100% inhibition from the inhibition studies with African bush mango fruit peel PPO [ 16 ]. Oxalic acid at 5 mM was defined as a medium effective inhibitor for fennel seed PPO with 41% inhibition [ 11 ], which had a stronger inhibitory effect than in the inhibition results of this study. The inhibition effect is due to its ability to change the pH of the reaction system, chelate the copper ion in the PPO active center, reduce the intermediates caused by enzyme browning, and transform light or colorless substances from the intermediates by combination.…”

Section: Resultsmentioning

confidence: 99%

“…The molecular weight of purified wheat PPO was estimated by SDS-PAGE according to the method of Karakus et al [ 11 ]. The purified PPO and Markers were mixed with the sample loading buffer at a ratio of 4:1 and incubated in boiling water for 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…Also, studies have shown that Cu 2+ ions inhibit the activity of soluble PPO from Prunus mume but activate that of membrane PPO [ 9 ]. There has been evidence that ascorbic acid and sodium metabisulfite inhibit PPO in several plants, including green beans [ 10 ], fennel seeds [ 11 ] , mamey [ 12 ], and truffles [ 13 ]. Hence, the mutual reference information on different purified PPO properties is not applicable to a wide range of applications; therefore, research on the enzymatic properties of wheat PPO is essential for regulating wheat products’ browning or color changes.…”

Section: Introductionmentioning

confidence: 99%

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Purification and Biochemical Characterization of Polyphenol Oxidase Extracted from Wheat Bran (Wan grano)

Yu,

He,

et al. 2024

Molecules

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Currently, little is known about the characteristics of polyphenol oxidase from wheat bran, which is closely linked to the browning of wheat product. The wheat PPO was purified by ammonium sulfate precipitation, DEAE-Sepharose ion-exchange column, and Superdex G-75 chromatography column. Purified wheat PPO activity was 11.05-fold higher, its specific activity was 1365.12 U/mg, and its yield was 8.46%. SDS-PAGE showed that the molecular weight of wheat PPO was approximately 21 kDa. Its optimal pH and temperature were 6.5 and 35 °C for catechol as substrate, respectively. Twelve phenolic substrates from wheat and green tea were used for analyzing the substrate specificity. Wheat PPO showed the highest affinity to catechol due to its maximum Vmax (517.55 U·mL−1·min−1) and low Km (6.36 mM) values. Docking analysis revealed strong affinities between catechol, gallic acid, EGCG, and EC with binding energies of −5.28 kcal/mol, −4.65 kcal/mol, −4.21 kcal/mol, and −5.62 kcal/mol, respectively, for PPO. Sodium sulfite, ascorbic acid, and sodium bisulfite dramatically inhibited wheat PPO activity. Cu2+ and Ca2+ at 10 mM were considered potent activators and inhibitors for wheat PPO, respectively. This report provides a theoretical basis for controlling the enzymatic browning of wheat products fortified with green tea.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Purification and Biochemical Characterization of Polyphenol Oxidase Extracted from Wheat Bran (Wan grano)

Yu,

He,

et al. 2024

Molecules

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“…Traditional Chinese medicine has a long history and plays an important role in treating human diseases. PPO is a dinuclear copper-centered enzyme, widely distributed in plants, fungi, microorganisms, and animals [ 47 ] as an antioxidant enzyme that can oxidize high phenol and non-phenol aromatic compounds [ 48 ]. In addition, it is involved in oxygen scavenging, pigment formation, and defense mechanisms against plant pathogens and herbivores [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy

et al. 2022

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Cancers have always been the most difficult to fight, the treatment of cancer is still not considered. Thus, exploring new anticancer drugs is still imminent. Traditional Chinese medicine has played an important role in the treatment of cancer. Polyphenol oxidase (PPO) extracted from Edible mushroom has many related reports on its characteristics, but its role in cancer treatment is still unclear. This study aims to investigate the effects of PPO extracted from Edible mushroom on the proliferation, migration, invasion, and apoptosis of cancer cells in vitro and explore the therapeutic effects of PPO on tumors in vivo. A cell counting kit-8 (CCK8) assay was used to detect the effect of PPO on the proliferation of cancer cells. The effect of PPO on cancer cell migration ability was detected by scratch test. The effect of PPO on the invasion ability of cancer cells was detected by a transwell assay. The effect of PPO on the apoptosis of cancer cells was detected by flow cytometry. Female BALB/c mice (18–25 g, 6–8 weeks) were used for in vivo experiments. The experiments were divided into control group, model group, low-dose group (25 mg/kg), and high-dose group (50 mg/kg). In vitro, PPO extracted from Edible mushroom significantly inhibited the proliferation, migration, and invasion capability of breast cancer cell 4T1, lung cancer cell A549, and prostate cancer cell C4-2, and significantly promoted the apoptosis of 4T1, A549, and C4-2. In vivo experiments showed PPO inhibitory effect on tumor growth. Collectively, the edible fungus extract PPO could play an effective role in treating various cancers, and it may potentially be a promising agent for treating cancers.

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“…The spectrophotometric approach established by Karakus et al (2021) 31 allows for the simultaneous assessment of the activity of polyphenol oxidases, including catechol oxidase and laccase.…”

Section: Polyphenol Oxidase (Ppo)mentioning

confidence: 99%

Assessment of In Vitro Antioxidant and Anti-Inflammatory Activities of Pumpkin (Cucurbita pepo) Natural Plant

Fathima,

Firdous,

Pal

et al. 2024

Natural Product Communications

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Objectives The objective of this research was to investigate the potential anti-inflammatory properties of the Cucurbita pepo fruit. Given the adverse effects associated with long-term use or excessive doses of conventional anti-inflammatory medications, exploring herbal therapies as alternatives has become increasingly important. Methods Various preliminary tests and antioxidant assays, both enzymatic and non-enzymatic, were conducted on the C . pepo fruit. Bioactive substances present in the fruit, including alkaloids, carbohydrates, flavonoids, tannins, amino acids, triterpenoids, and saponin glycosides, were identified through early analysis. Different extraction solvents, such as chloroform, methanol, and water, were utilized to extract samples. The methanolic extract was subjected to further testing for its in vitro anti-inflammatory characteristics. This involved evaluating their ability to stabilize RBC membranes, inhibit protein denaturation, and suppress proteinase activity using two concentrations (150 µg/ml and 300 µg/ml) of the extract. Results The methanolic extract of the C. pepo fruit exhibited strong free radical scavenging activity in both DPPH and H2O2 assays. Moreover, it demonstrated the dose-dependent stabilization of RBC membranes, resulting in reduced hemolysis, protein denaturation, and proteinase activity. These findings suggest potent anti-inflammatory effects of the methanolic extract. Conclusion This research study has found that the C. pepo fruit methanolic extract significantly reduced inflammation. This demonstrates the promise of natural chemicals as less risky substitutes for traditional anti-inflammatory medications. Potentially safe and efficient treatments for inflammatory illnesses could be found in herbal remedies such as the C. pepo fruit. Therefore, this study highlights the significance of exploring herbal remedies as a potential treatment option for inflammation-related diseases.

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Corrigendum to “Characterization of polyphenol oxidase from Fennel (Foeniculum vulgare Mill.) seeds as a promising source” [Int. J. Biol. Macromol. 170 (2021) 261–271]

Cited by 3 publications

References 0 publications

Purification and Biochemical Characterization of Polyphenol Oxidase Extracted from Wheat Bran (Wan grano)

Purification and Biochemical Characterization of Polyphenol Oxidase Extracted from Wheat Bran (Wan grano)

Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy

Assessment of In Vitro Antioxidant and Anti-Inflammatory Activities of Pumpkin (Cucurbita pepo) Natural Plant

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