Purification and Biochemical Characterization of Polyphenol Oxidase Extracted from Wheat Bran (Wan grano)
Kun Yu,
Wei He,
Xiaoli Ma
et al.
Abstract:Currently, little is known about the characteristics of polyphenol oxidase from wheat bran, which is closely linked to the browning of wheat product. The wheat PPO was purified by ammonium sulfate precipitation, DEAE-Sepharose ion-exchange column, and Superdex G-75 chromatography column. Purified wheat PPO activity was 11.05-fold higher, its specific activity was 1365.12 U/mg, and its yield was 8.46%. SDS-PAGE showed that the molecular weight of wheat PPO was approximately 21 kDa. Its optimal pH and temperatur… Show more
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