Corrigendum: The Polyphenol Pterostilbene Ameliorates the Myopathic Phenotype of Collagen VI Deficient Mice via Autophagy Induction

Metti, Samuele; Gambarotto, Lisa; Chrisam, Martina; Spina, Martina La; Baraldo, Martina; Braghetta, Paola; Blaauw, Bert; Bonaldo, Paolo

doi:10.3389/fcell.2020.615542

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“…In Col6 knockout muscles, increased mitochondrial intima permeability transition pore (PTP) openings are thought to contribute to the muscular dystrophy phenotype ( Angelin et al, 2007 ; Bernardi and Bonaldo, 2008 ). In addition, other studies have shown that the mitochondrial changes in Col6a1 −/− mouse muscles are caused by the regulatory defects of the autophagy pathway, and reactivation of the autophagy pathway through drug, genetic or nutritional pathways can rescue the dysfunction of organelles, inhibit apoptosis and prevent the decline of muscle strength ( Grumati et al, 2010 ; Chrisam et al, 2015 ; Metti et al, 2021 ). Since the absence of Col6 can lead to muscle dysfunction or muscular atrophy ( Di Martino A et al, 2023 ), it suggests that Col6 may be an important target for improving ventilator diaphragm dysfunction in diabetic mice.…”

Section: Discussionmentioning

confidence: 99%

Identification of key genes affecting ventilator-induced diaphragmatic dysfunction in diabetic mice

Xing,

Yu,

et al. 2024

Front. Genet.

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BackgroundMechanical ventilation (MV) is often required in critically ill patients. However, prolonged mechanical ventilation can lead to Ventilator-induced diaphragmatic dysfunction (VIDD), resulting in difficulty in extubation after tracheal intubation, prolonged ICU stay, and increased mortality. At present, the incidence of diabetes is high in the world, and the prognosis of diabetic patients with mechanical ventilation is generally poor. Therefore, the role of diabetes in the development of VIDD needs to be discovered.MethodsMV modeling was performed on C57 mice and DB mice, and the control group was set up in each group. After 12 h of mechanical ventilation, the muscle strength of the diaphragm was measured, and the muscle fiber immunofluorescence staining was used to verify the successful establishment of the MV model. RNA sequencing (RNA-seq) method was used to detect mRNA expression levels of the diaphragms of each group, and then differential expressed gene analysis, Heatmap analysis, WGCNA analysis, Venn analysis, GO and KEGG enrichment analysis were performed. qRT-PCR was used to verify the expression of the selected mRNAs.ResultsOur results showed that, compared with C57 control mice, the muscle strength and muscle fiber cross-sectional area of mice after mechanical ventilation decreased, and DB mice showed more obvious in this respect. RNA-seq showed that these differential expressed (DE) mRNAs were mainly related to genes such as extracellular matrix, collagen, elastic fiber and Fbxo32. GO and KEGG enrichment analysis showed that the signaling pathways associated with diabetes were mainly as follows: extracellular matrix (ECM), protein digestion and absorption, PI3K-Akt signaling pathway, calcium signaling pathway, MAPK signaling pathway and AGE-RAGE signaling pathway in diabetic complications, etc. ECM has the closest relationship with VIDD in diabetic mice. The key genes determined by WGCNA and Venn analysis were validated by quantitative real-time polymerase chain reaction (qRT-PCR), which exhibited trends similar to those observed by RNA-seq.ConclusionVIDD can be aggravated in diabetic environment. This study provides new evidence for mRNA changes after mechanical ventilation in diabetic mice, suggesting that ECM and collagen may play an important role in the pathophysiological mechanism and progression of VIDD in diabetic mice, and provides some clues for the research, diagnosis, and treatment of VIDD in diabetic context.

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