Corrigendum: Genome-wide association study of esophageal squamous cell carcinoma in Chinese subjects identifies susceptibility loci at PLCE1 and C20orf54

Wang, Lidong; Zhou, Fuyou; Li, Xuemin; Sun, Liangdan; Song, Xin; Jin, Yan; Jiang-man, LI; Kong, Guo-Qiang; Qi, Hong; Cui, Juan; Zhang, Lian-Qun; Yang, Jiezhi; Li, Jilin; Li, Xing-Chuan; Ren, Jingli; Liu, Zhicai; Gao, Wenjun; Yuan, Ling; Wu, Wei; Zhang, Yanrui; Wang, Weipeng; Sheyhidin, Ilyar; Li, Feng; Chen, Bao-Ping; Ren, Shu-Wei; Liu, Bin; Li, Dan; Ku, Jian-Wei; Fan, Zong-Min; Zhou, Shengli; Guo, Zhigang; Zhao, Xueke; Liu, Na; Ai, Yong-hong; Shen, Fangfang; Cui, Wenyan; Song, Shuang; Guo, Tao; Huang, Jing; Yuan, Chao; Huang, Jia; Yi-ming, WU; Yue, Wenbin; Chen, Feng; Li, Honglei; Wang, Yan; Tian, Jin-Ya; Lu, Yao; Yuan, Yi; Zhu, Wenliang; Liu, Min; Fu, Wenjing; Xia, Yang; Wang, Han-Jing; Han, Suo-Li; Chen, Jie; Han, Moonsik; Wang, Haiyan; Zhang, Peng; Li, Xiumin; Dong, Jin‐Cheng; Xing, Guo-Lan; Wang, Ran; Guo, Ming; Chang, Zhiwei; Liu, Hailin; Guo, Li; Yuan, Zhiqing; Liu, Hai; Lu, Qiumin; Yang, Liu-Qin; Zhu, Fu-Guo; Yang, Xiufeng; Feng, Xiaoshan; Wang, Zhou; Yin, Li; Gao, Shegan; Qige, Qirenwang; Bai, Long-Tang; Yang, Wenjun; Lei, Guangyan; Shen, Zhong-Ying; Chen, Long-Qi; Li, En‐Min; Xu, Li; Wu, Zhi‐Yong; Cao, Wei-Ke; Wang, Jianpo; Bao, Zhi-Qin; Chen, Ji‐Li; Ding, Guang-Cheng; Zhuang, Xiang; Zhou, Yingfa; Zheng, Hou-Feng; Zhang, Zheng; Zuo, Xianbo; Dong, Ziming; Fan, Daiming; He, Xin; Wang, Jin; Zhou, Qi; Zhang, Qinxian; Jiao, Xin-Ying; Lian, Sen; Ji, Ai‐Fang; Lü, Xiaomei; Wang, Jinsheng; Chang, Fu-Bao; Chen, Lu; Chen, Zhiguo; Miao, Jianjun; Zhang, Fan; Lin, Ruobai; Liu, Tai-Jiang; Wei, Jin-Chang; Kong, Qing‐Peng; Lan, Yu Ching; Fan, Yu-Jing; Gao, Fusheng; Wang, Tianyun; Xie, Dong; Chen, Shuqing; Yang, Wancai; Hong, Jun-Yan; Wang, Liang; Qiu, Song-Liang; Cai, Zhiming; Zhang, Xuejun

doi:10.1038/ng0914-1041

Cited by 88 publications

(169 citation statements)

References 2 publications

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“…It is very important to explore the molecular mechanism. In 2010, by genomewide association study (GWAS) of 25,000 patients for the susceptibility loci to esophageal squamous cell carcinoma (ESCC) and health controls, we firstly identified PLCE1 (phospholipase Cε1, 10q23) and C20orf54 as the susceptibility loci to ESCC (Wang et al, 2010). Our previous study showed that the functional SNP in C20orf54 may modifiy susceptibility to esophageal squamous cell carcinoma (Ji et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Effects of PLCE1 Gene Silencing by RNA Interference on Cell Cycling and Apoptosis in Esophageal Carcinoma Cells

Zhao¹,

Wei²,

Yang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Esophageal squamous cell carcinoma (ESCC) is one of the most malignancies with a poor prognosis. The phospholipase Cε gene (PLCE1) encodes a novel ras-related protein effector mediating the effects of R-Ras on the actin cytoskeleton and membrane protrusion. However, molecular mechanisms pertinent to ESCC are unclear. We therefore designed PLCE1-special small interfering RNA and transfected to esophageal squamous cell (EC) 9706 cells to investigat the effects of PLCE1 gene silencing on the cell cycle and apoptosis of ESCC and indicate its important role in the development of ESCC. Esophageal cancer tissue specimens and normal esophageal mucosa were obtained and assayed by immunohistochemical staining to confirm overexpression of PLCE1 in neoplasias. Fluorescence microscopy was used to examine transfection efficiency, while the result of PLCE1 silencing was examined by reverse transcription (RT-PCR). Flow cytometry and annexin V apoptosis assays were used to assess the cell cycle and apoptosis, respectively. Expression of cyclin D1 and caspase-3 was detected by Western-blotting. The level of PLCE1 protein in esophageal cancer tissue was significantly higher than that in normal tissue. After transfection, the expression of PLCE1 mRNA in EC 9706 was significantly reduced, compared with the control group. Furthermore, flow cytometry results suggested that the PLCE1 gene silencing arrested the cell cycle in the G0/G1 phase; apoptosis was significantly higher than in the negative control group and mock group. PLCE1 gene silencing by RNAi resulted in decreased expression of cyclin D1 and increased expression of caspase-3. Our study suggests that PLCE1 may be an oncogene and play an important role in esophageal carcinogenesis through regulating proteins which control cell cycling and apoptosis.

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Section: Discussionmentioning

confidence: 99%

“…These products initiate a cascade of intracellular responses that result in cell growth and differentiation and gene expression. Wang found that PLCE1 is correlated with the occurrence and development of ESCC (Wang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effects of PLCE1 Gene Silencing by RNA Interference on Cell Cycling and Apoptosis in Esophageal Carcinoma Cells

Zhao¹,

Wei²,

Yang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Three large-scale and independent genome-wide association studies (GWAS) in China recently reported that a new susceptibility locus (rs2274223: A5780G), located in exon 26 of Phospholipase C epsilon 1 (PLCE1) is strongly associated with the risk of esophageal and gastric cancers in Chinese population (Abnet et al, 2010;Wang et al, 2010;. PLCE1, which is located in chromosome 10q23, encodes a phospholipase that hydrolyzes phosphatidyl-inositol 4,5-bisphosphate to 1,2-diacylglycerol and inositol 1, 4, 5-trisphosphate (Wing et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Cui

Peng²,

Su³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Genome-wide association studies (GWAS) is a powerful tool for identifying genetic markers for various diseases with high-throughput genotyping technology (2). Recent GWAS identified susceptibility loci for esophageal squamous cell carcinoma (ESCC), the most common histological type of esophageal cancer, in the phospholipase C ε-1 gene (PLCE1) (3)(4)(5). Subsequently, several independent studies were conducted to validate the association between PLCE1 single-nucleotide polymorphisms (SNPs) and ESCC, with controversial results (6)(7)(8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Phospholipase C ε‑1 gene polymorphisms and prognosis of esophageal cancer patients from a high‑incidence region in northern China

Zhou

Wang

et al. 2017

mol clin onc

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Abstract. Recent genome-wide association studies identified susceptibility loci for esophageal squamous cell carcinoma (ESCC), the most common histological type of esophageal cancer, in the phospholipase C ε-1 gene (PLCE1). The aim of the present study was to investigate whether polymorphisms of PLCE1 were associated with the prognosis of ESCC patients in a high-incidence region of northern China. The PLCE1 rs2274223 A/G and rs11599672T/G single-nucleotide polymorphisms (SNPs) were genotyped by polymerase chain reaction-ligase detection reaction method in 207 ESCC patients with survival information. The mean age ± standard deviation of the 207 ESCC patients was 60.3±7.9 years. Sex, age, smoking status and family history of upper gastrointestinal cancer were not found to be associated with the survival time of ESCC patients. The mean survival time of rs2274223

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Corrigendum: Genome-wide association study of esophageal squamous cell carcinoma in Chinese subjects identifies susceptibility loci at PLCE1 and C20orf54

Cited by 88 publications

References 2 publications

Effects of PLCE1 Gene Silencing by RNA Interference on Cell Cycling and Apoptosis in Esophageal Carcinoma Cells

Effects of PLCE1 Gene Silencing by RNA Interference on Cell Cycling and Apoptosis in Esophageal Carcinoma Cells

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Phospholipase C ε‑1 gene polymorphisms and prognosis of esophageal cancer patients from a high‑incidence region in northern China

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