Corrigendum: Extended-spectrum β-lactamase-producing and carbapenemase-producing Enterobacteriaceae

Wilson, Hayley; Török, M. Estée

doi:10.1099/mgen.0.000218

Cited by 5 publications

(4 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fluoroquinolone and rifamycin resistance was also mainly determined by a single substitution in GyrA (Thr82Ile) and RpoB (Arg505Lys), respectively. This is similar to other Gram-positive bacteria, such as Staphylococcus aureus [ 46 ], where one genotypic determinant is responsible for a resistance phenotype in a majority of the bacterial population and is in contrast to many Gram-negative bacteria, such as several members in the Enterobacteriaceae [ 47 ], where resistance to an antimicrobial class can be conferred by several genotypic determinants. The dominance of a single genotypic determinant accommodates the development of genotype-based rapid detection kits for drug-resistant C. difficile , similar to real-time PCR assays for methicillin-resistant S. aureus [ 48 ].…”

Section: Discussionmentioning

confidence: 58%

A species-wide genetic atlas of antimicrobial resistance in Clostridioides difficile

et al. 2021

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) plays an important role in the pathogenesis and spread of Clostridioides difficile infection (CDI), the leading healthcare-related gastrointestinal infection in the world. An association between AMR and CDI outbreaks is well documented, however, data is limited to a few ‘epidemic’ strains in specific geographical regions. Here, through detailed analysis of 10 330 publicly-available C. difficile genomes from strains isolated worldwide (spanning 270 multilocus sequence types (STs) across all known evolutionary clades), this study provides the first species-wide snapshot of AMR genomic epidemiology in C. difficile . Of the 10 330 C . difficile genomes, 4532 (43.9 %) in 89 STs across clades 1–5 carried at least one genotypic AMR determinant, with 901 genomes (8.7 %) carrying AMR determinants for three or more antimicrobial classes (multidrug-resistant, MDR). No AMR genotype was identified in any strains belonging to the cryptic clades. C. difficile from Australia/New Zealand had the lowest AMR prevalence compared to strains from Asia, Europe and North America (P<0.0001). Based on the phylogenetic clade, AMR prevalence was higher in clades 2 (84.3 %), 4 (81.5 %) and 5 (64.8 %) compared to other clades (collectively 26.9 %) (P<0.0001). MDR prevalence was highest in clade 4 (61.6 %) which was over three times higher than in clade 2, the clade with the second-highest MDR prevalence (18.3 %). There was a strong association between specific AMR determinants and three major epidemic C. difficile STs: ST1 (clade 2) with fluoroquinolone resistance (mainly T82I substitution in GyrA) (P<0.0001), ST11 (clade 5) with tetracycline resistance (various tet-family genes) (P<0.0001) and ST37 (clade 4) with macrolide-lincosamide-streptogramin B (MLSB) resistance (mainly ermB) (P<0.0001) and MDR (P<0.0001). A novel and previously overlooked tetM-positive transposon designated Tn6944 was identified, predominantly among clade 2 strains. This study provides a comprehensive review of AMR in the global C. difficile population which may aid in the early detection of drug-resistant C. difficile strains, and prevention of their dissemination worldwide.

show abstract

Section: Discussionmentioning

confidence: 58%

A species-wide genetic atlas of antimicrobial resistance in Clostridioides difficile

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Indeed, carbapenems have often been used as the treatment of choice for infectious diseases due to ESBL-PE. This has led to the emergence of carbapenemase-producing Enterobacteriaceae (CPE), which are enzymes hydrolysing all beta-lactams, even carbapenems which are the last line in the treatment for ESBL-PE [7,8]. There are limited data assessing the impact of different factors on the current rate of antimicrobial resistance in low-income countries [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of antimicrobial resistance in central africa: A systematic review

Dikoumba,

Onanga,

Mangouka

et al. 2023

Access Microbiology

View full text Add to dashboard Cite

Background. In Central Africa, it is difficult to tackle antibiotic resistance, because of a lack of data and information on bacterial resistance, due to the low number of studies carried out in the field. To fill this gap, we carried out a systematic review of the various studies, and devised a molecular epidemiology of antimicrobial resistance from humans, animals and the environmental samples. Method. A systematic search of all publications from 2005 to 2020 on bacterial resistance in Central Africa (Gabon, Cameroon, Democratic Republic of Congo, Central African Republic, Chad, Republic of Congo, Equatorial Guinea, São Tomé and Príncipe, Angola) was performed on Pubmed, Google scholar and African Journals Online (AJOL). All circulating resistance genes, prevalence and genetic carriers of these resistances were collected. The study area was limited to the nine countries of Central Africa. Results. A total of 517 studies were identified through a literature search, and 60 studies carried out in eight countries were included. Among all articles included, 43 articles were from humans. Our study revealed not only the circulation of beta-lactamase and carbapenemase genes, but also several other types of resistance genes. To finish, we noticed that some studies reported mobile genetic elements such as integrons, transposons, and plasmids. Conclusion. The scarcity of data poses difficulties in the implementation of effective strategies against antibiotic resistance, which requires a health policy in a ‘One Health’ approach.

show abstract

“…For example, ESBL-producing E. coli (ESBL-EC) have been detected in the influent and effluent of WWTPs and the receiving surface waters [ 19 ]. It is known that human infections with ESBL-EC or carbapenem-resistant Enterobacteriaceae (CPE) are associated with increased mortality rates, time to effective therapy, length of hospital stay, and overall healthcare costs [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance in Wastewater Treatment Plants and Transmission Risks for Employees and Residents: The Concept of the AWARE Study

et al. 2021

View full text Add to dashboard Cite

Antibiotic resistance has become a serious global health threat. Wastewater treatment plants may become unintentional collection points for bacteria resistant to antimicrobials. Little is known about the transmission of antibiotic resistance from wastewater treatment plants to humans, most importantly to wastewater treatment plant workers and residents living in the vicinity. We aim to deliver precise information about the methods used in the AWARE (Antibiotic Resistance in Wastewater: Transmission Risks for Employees and Residents around Wastewater Treatment Plants) study. Within the AWARE study, we gathered data on the prevalence of two antibiotic resistance phenotypes, ESBL-producing E. coli and carbapenemase-producing Enterobacteriaceae, as well as on their corresponding antibiotic resistance genes isolated from air, water, and sewage samples taken from inside and outside of different wastewater treatment plants in Germany, the Netherlands, and Romania. Additionally, we analysed stool samples of wastewater treatment plant workers, nearby residents, and members of a comparison group living ≥1000 m away from the closest WWTP. To our knowledge, this is the first study investigating the potential spread of ESBL-producing E. coli, carbapenemase-producing Enterobacteriaceae, and antibiotic resistance genes from WWTPs to workers, the environment, and nearby residents. Quantifying the contribution of different wastewater treatment processes to the removal efficiency of ESBL-producing E. coli, carbapenemase-producing Enterobacteriaceae, and antibiotic resistance genes will provide us with evidence-based support for possible mitigation strategies.

show abstract

Corrigendum: Extended-spectrum β-lactamase-producing and carbapenemase-producing Enterobacteriaceae

Cited by 5 publications

References 1 publication

A species-wide genetic atlas of antimicrobial resistance in Clostridioides difficile

A species-wide genetic atlas of antimicrobial resistance in Clostridioides difficile

Molecular epidemiology of antimicrobial resistance in central africa: A systematic review

Antibiotic Resistance in Wastewater Treatment Plants and Transmission Risks for Employees and Residents: The Concept of the AWARE Study

Contact Info

Product

Resources

About