Correspondence

Dj, Baker; Cd, Town

doi:10.1259/0007-1285-39-465-706

Cited by 24 publications

(2 citation statements)

References 6 publications

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“…The diffusion rate was found to be very low, especially when growth medium was used . Furthermore, the presence of cells in the growth medium did not seem to have any effect on the diffusion rate (Baker and Town 1966) .…”

Section: 1 Cell Culture Techniquementioning

confidence: 85%

See 1 more Smart Citation

X-ray Inactivation of Human Cells in Tissue Culture under Aerobic and Extremely Hypoxic Conditions in the Presence and Absence of TMPN

Pettersen

Oftebro

Brustad

1973

International Journal of Radiation Biology and Related Studies

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A technique is described which permits irradiation of suspensions of human cells in tissue culture either aerobically (equilibrated with air) or under extremely hypoxic conditions (flushed with a mixture of 97 per cent N 2 and 3 per cent C0 2i the oxygen content of which was less than 15 p .p .m .) .An established cell-line, NHIK 3025, originally derived from an early stage of human cancer of the cervix, was used . The D o value of these cells under aerobic conditions was 130 rads and the extrapolation number about 3 . Under extremely hypoxic conditions, the dose-effect curve was exponential with a Do value of 600 rads . The o .e .r . derived from the ratio of the D o values was therefore 4. 6 .2,2,6,6,tetramethyl-4-piperidinol-N-oxyl (TMPN) at a concentration of 10 -2 M exerted a pronounced sensitizing effect under extremely hypoxic conditions (TMPN e .r .=2 . 5), and yielded dose-effect curves with an extrapolation number of 3 . The high radio-resistance of the cell under extremely hypoxic conditions in the absence of TMPN may be relevant to the evaluation of the radio-curability of tumours such as squamous cell carcinoma of the cervix uteri .

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Section: 1 Cell Culture Techniquementioning

confidence: 85%

“…Both Baker and Town (1966) and Michael, Scott and Revesz (1966) have presented results from measurements on the diffusion of oxygen from an unstirred liquid to a gas phase containing less than 100 p .p .m . of oxygen 35 min after the flushing was started .…”

Section: 1 Cell Culture Techniquementioning

confidence: 99%