Correlative light and electron microscope study of the basement membrane of the human ectocervix

Younes, M.S.; Steele, H. D.; Robertson, Edwin M.; Bencosme, Sergio A.

doi:10.1016/s0002-9378(65)80001-1

Cited by 37 publications

(16 citation statements)

References 14 publications

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“…At high magnification, it appears to be traversed by fine filamentousappearing densities extending from the plasma membrane to the basal lamina ( 5). These densities are very similar to the bridging fibrils described in other 35 and can be seen along both epithelial and myoepithelial cells. They seem to be continuous with the substance of the basal lamina on one side and the plasma membrane on the other, even in areas devoid of hemidesmosomes, but no proof can be found of their being continuous with intracytoplasmic filaments or of representing extracellular extensions of these filaments.…”

supporting

confidence: 82%

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Epithelial-stromal junction of normal and dysplastic mammary glands

Ozzello

1970

Cancer

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This report illustrates the electron microscopic features of the constituents of that portion of the human breast in which epithelial structures and stroma come in contact with each other. This zone is designated “epithelial‐stromal junction” (ESJ) and includes: plasma membranes of epithelial and myoepithelial cells, intercellular spaces, lamina lucida, basal lamina, fibrillar connective tissue and ground substance beneath the basal lamina, and a layer of “delimiting fibroblasts.” The latter make an almost continuous layer of attenuated cytoplasm enveloping ducts and ductules. Vascular channels are present beyond the layer of delimiting fibroblasts but not within the ESJ proper. Several ultrastructural alterations of the ESJ are observed in the various manifestations of mammary dysplasia. These alterations include: straightening of the plasma membranes with flattening of hemidesmosomes, straightening with variations in thickness but no disappearance of basal laminae, increased number of elastic fibers, increased number and compactness of collagen fibers, irregularly increased density of interfibrillary substance, disarray and partial‐to‐total loss of the layer of delimiting fibroblasts. These observations lead to the hypothesis that the ESJ probably acts as a functional unit in the transport of materials to and from the epithelium thus taking an active part in the normal functions of the mammary gland and in the evolution of mammary dysplasia.

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confidence: 82%

“…Only once, a few structures resembling the anchoring fibers described in other locations25. 29, 35 could be seen oriented perpendicularly to the basal lamina and seemingly merging with it ( Fig. 5).…”

mentioning

confidence: 99%

Epithelial-stromal junction of normal and dysplastic mammary glands

Ozzello

1970

Cancer

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“…The basement membrane has been considered to be a condensation of reticulin fibres beneath the epithelium (Younes et al, 1965), but the demonstration of such a continuous condensation of reticulin fibres is difficult to evidence by conventional histological techniques (Lamb et al, 1960). However, Beutner's studies (1968) have demonstrated the presence of particular antigens in a basement membrane-like pattern beneath the squamous epithelium, which are defined by their reaction with antibodies occurring in patients with bullous pemphigoid.…”

Section: Discussionmentioning

confidence: 99%

The basement membrane in experimentally induced atypias and carcinoma of the uterine cervix in mice

Rubio

Biberfeld

1979

Virchows Arch. A Path. Anat. and Histol.

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Basement membrane-specific antigens of the squamous epithelium of the uterine cervix were investigated in 19 normal mice, in 7 mice with cervical atypia and in 3 mice with invasive carcinoma. Cervical atypia and carcinoma were induced by local application of benzo(a)pyrene. Basement membrane-specific antigens were demonstrated by immunofluorescence with sera from patients with bullous pemphigoid. Both normal squamous cervical epithelium and atypical cervical epithelium showed the presence of a continuous, clearly delineated basement membrane. Clusters of invasive squamous carcinoma were also surrounded by a fluorescent basement membrane which, however, appear fragmented or discontinuous. The results suggest that the ability of cervical squamous cells to secrete basement membrane antigens is not completely lost during carcinogenesis, thus substantiating our previous observations in the cervix of human subjects.

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“…However, some reactive material in the epithelial "basement membrane" always survived digestion, suggesting that it was bound to substances which were not trypsin labile. Collagen may fill this role, and this suggestion is supported by the electron microscopic histochemical investigations of Younes et al (1965) and Swift and Saxton (1967). Alternatively, some trypsinresistant, or relatively resistant, non-collagenous protein or peptide, such as is found in proteolipids (Folch and Lees 1951), may be involved.…”

Section: (Ii) Distribution Of Mucosubstances In Gingivamentioning

confidence: 95%

Some histological and histochemical observations on the connective tissue of chronically inflamed human gingiva

Melcher

1967

J of Periodontal Research

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This investigation was undertaken during the tenure of a Research Fellowship in the Institute of Dental Surgery, University of London, and a Leverhulme Research Fellowship in the Department of Dental Science, Royal College of Surgeons of England. Some of the findings were included in a Thesis submitted to the University of London for the Degree of Doctor of Philosophy. One hundred and fifty biopsies of human gingiva, removed randomly from patients undergoing gingivectomy, were examined in the light microscope. Cold microtome and paraffin sections were prepared. Eosin, Mallory's PTAH, acid solochrome cyanine and chlorantine fast red, the Gomori aldehyde fuchsin, resorcin fuchsin and orcein methods for elastic fibres, and silver impregnation were used to colour collagen fibres; fibrin was stained by the DMAB method, and mucosubstances by the paS, DMPD, alcian blue and the aldehyde fuchsin methods. All the interdental gingival septa exhibited an ulcerated col. The intercellular substance of the connective tissue was not separated in cold microtome sections, as in paraffin sections, and probably more faithfully reflected the state of the tissue in vivo. However, cell detail in paraffin sections was clearer than in cold microtome sections. The collagen fibres in inflamed connective tissue were found to disintegrate and to lose their affinity for stains with which they usually react, but were not coloured by stains for elastic fibres during the process, nor by the DMAB method. The distribution of neutral and acid mucosubstances was found to overlap in healthy connective tissue. Neutral mucosubstances became less reactive in inflamed areas, but the reactivity of acidic mucopolysaccharides, which could also be demonstrated by two of the three stains used for elastic fibres, increased at the periphery of these areas, and was thought to be associated with fibrogenesis.

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Correlative light and electron microscope study of the basement membrane of the human ectocervix

Cited by 37 publications

References 14 publications

Epithelial-stromal junction of normal and dysplastic mammary glands

Epithelial-stromal junction of normal and dysplastic mammary glands

The basement membrane in experimentally induced atypias and carcinoma of the uterine cervix in mice

Some histological and histochemical observations on the connective tissue of chronically inflamed human gingiva

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