Correlative analysis of nanoparticle tracking, flow cytometric and functional measurements for circulating microvesicles in normal subjects

Enjeti, Anoop K; Ariyarajah, Anita; D'Crus, Angel; Seldon, Michael; Lincz, Lisa F.

doi:10.1016/j.thromres.2016.06.029

Cited by 18 publications

(16 citation statements)

References 34 publications

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“…Measurements were taken in triplicate for analysis using the Nanosight software (version 2.3 and 3.1) in scatter or fluorescent mode. A comparative analysis of the various techniques used in this study has been recently published [11].…”

Section: Nanoparticle Tracking Analysis (Nta)mentioning

confidence: 99%

Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles

Enjeti

Ariyarajah

D'Crus

et al. 2017

Thrombosis Research

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Section: Nanoparticle Tracking Analysis (Nta)mentioning

confidence: 99%

Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles

Enjeti

Ariyarajah

D'Crus

et al. 2017

Thrombosis Research

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“…A combination of optical or non-optical enumeration as well as functional methods may be required for a complete profiling of circulating MPs [32]. There is large body evidence regarding that the combination of SPR or RPS methods to atomic force microscopy or coupling NTA with Raman microscopy, microbeads and fluorescence quantum dots exhibited much more accurate ability to detect both number and structure of MPs when compared with traditional flow cytometry and fluorescent microscopy [33][34][35].…”

Section: Couple Methods For Mp Identificationmentioning

confidence: 99%

Coupling Analytical Methods for Detection of Microparticles: The Possibilities for Improvement

Berezin¹

2017

J Biotechnol Biomater

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Microparticles (MPs) are considered important diagnostic biological markers in many diseases with promising predictive value. There are several methods that currently used for the detection of number and characterization of structure and features of MPs. Therefore, the MP detection methods have been remained pretty costly and time consuming. The review is depicted the perspectives to use coupling methods for MP measurement and structure assay. Indeed, there is large body evidence regarding that the combination of atomic force microscopy or coupling nanoparticle tracking analysis (NTA) with microbeads, plasmon resonance method and fluorescence quantum dots could exhibit much more accurate ability to detect both number and structure of MPs when compared with traditional flow cytometry and fluorescent microscopy. Whether several combined methods would be useful for advanced MP detection is not fully clear, while it is extremely promising.

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“…They reported that EVs added to re-calcified platelet-poor plasma decreased the clotting time and shortened the lag time and the time to peak using calibrated automated thrombography. Thrombin generation measured using a procoagulant activity assay and flow cytometry for phosphatidylserine and tissue factor-expressing EV are also reportedly well correlated [ 99 ].…”

Section: Main Textmentioning

confidence: 99%

Role of extracellular vesicles in the development of sepsis-induced coagulopathy

Iba

Ogura

2018

j intensive care

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BackgroundThe advances of research on extracellular vesicles (EVs) are of particular interest to the clinicians as well as the researchers who are studying coagulation disorder in sepsis. Here, we intend to update the latest knowledge and currently unsolved problems that should be addressed.Main bodySecreted membrane-enclosed vesicles including apoptotic bodies, exosomes, ectosomes, microvesicles, and microparticles are generically called EVs. Though the basic structure of these vesicles is the same, i.e., originating from the plasma membrane, their characteristics differ significantly depending on their surface structures and interior components. Numerous studies have shown elevated levels of circulating EVs that exhibit proinflammatory and procoagulant properties during sepsis. These EVs are known to play important roles in the development of coagulation disorder and organ dysfunction in sepsis. Coagulation disorder in sepsis is characterized by activated coagulation, disrupted anticoagulant systems, and imbalanced fibrinolytic systems. These processes collaborate with one another and contribute to the development of disseminated intravascular coagulation (DIC), with devastating consequences. As part of this pathogenesis, the membrane-exposed tissue factor, phosphatidylserine and bioactive substances contained within the vesicles, such as histones, nucleosomes, and high-mobility group box 1, contribute to the development of DIC. EVs not only upregulate the procoagulant systems by themselves, but they also disseminate prothrombotic activities by transferring their procoagulant properties to distant target cells. Though the basic concept behind the role of procoagulant properties, EVs in the development of sepsis-induced coagulopathy has started to be unveiled, knowledge of the actual status is far from satisfactory, mainly because of the lack of standardized assay procedures. Recent advances and current problems that remain to be resolved are introduced in this review.ConclusionThe recent studies succeeded to elucidate the important roles of EVs in the progress of coagulation disorder in sepsis. However, further harmonization in terminology, methodology, and evaluation methods is required for future studies.

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Correlative analysis of nanoparticle tracking, flow cytometric and functional measurements for circulating microvesicles in normal subjects

Cited by 18 publications

References 34 publications

Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles

Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles

Coupling Analytical Methods for Detection of Microparticles: The Possibilities for Improvement

Role of extracellular vesicles in the development of sepsis-induced coagulopathy

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