Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles

Enjeti, Anoop K; Ariyarajah, Anita; D'Crus, Angel; Seldon, Michael; Lincz, Lisa F.

doi:10.1016/j.thromres.2017.04.019

Cited by 36 publications

(49 citation statements)

References 21 publications

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“…Badrnya, Baumgartner, and Assinger (2014) determined the concentration of TF + EV in plasma of young healthy smokers and nonsmokers and found no significant difference. In another study with a broader age range, Enjeti et al (2017) reported that circulating TF + EV were decreased in smokers compared to nonsmokers using univariate analysis, whereas there was a significant positive correlation for smoking status and TF + EV after correcting for age. This highlights the importance of correcting for confounding factors in translational studies.…”

Section: In Vivo Evidence For Tf + Extracellular Vesiclesmentioning

confidence: 92%

“…In this human observational cohort, short-term PM exposure (hours to days) was associated with reduced, and long-term exposure (3 months to 1 year) with elevated concentrations of circulating PS + EV (Emmerechts et al 2012b). For CS exposure, while all in vitro studies reported increased levels of PS + EV (Cordazzo et al 2014;Li et al 2010;Serban et al 2016), all in vivo studies reported decreased concentrations in the blood circulation of smokers compared to nonsmokers (Badrnya, Baumgartner, and Assinger 2014;Enjeti et al 2017;Grant et al 2011). Whether cells indeed mount a different EV response to CS in vitro than in their physiological environment or whether the divergent findings are explained by other factors remains unclear.…”

Section: In Vivo Evidence For Ps + Extracellular Vesiclesmentioning

confidence: 99%

“…Several human and animal studies that assessed in vivo effects of respiratory exposures were not able to link changes in circulating EV to alterations in inflammatory markers (Emmerechts et al 2012a(Emmerechts et al , 2012bLevanen et al 2013;Mobarrez et al 2014). Several investigators even found that smokers displayed decreased concentrations of circulating PS + EV compared to nonsmokers (Badrnya, Baumgartner, and Assinger 2014;Enjeti et al 2017;Grant et al 2011). In part, the failure to detect proinflammatory changes in EV in response to respiratory toxicants in vivo may be due to (1) use of relatively mild and acute exposures (Levanen et al 2013;Mobarrez et al 2014) or (2) the fact that current methods are not sensitive enough to detect subtle changes in EV and inflammatory mediators in study populations without clinical disease.…”

Section: Conflicting Datamentioning

confidence: 99%

See 2 more Smart Citations

Extracellular vesicles released in response to respiratory exposures: implications for chronic disease

Benedikter

Wouters

Savelkoul

et al. 2018

Journal of Toxicology and Environmental Health, Part B

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Extracellular vesicles (EV) are secreted signaling entities that enhance various pathological processes when released in response to cellular stresses. Respiratory exposures such as cigarette smoke and air pollution exert cellular stresses and are associated with an increased risk of several chronic diseases. The aim of this review was to examine the evidence that modifications in EV contribute to respiratory exposure-associated diseases. Publications were searched using PubMed and Google Scholar with the search terms (cigarette smoke OR tobacco smoke OR air pollution OR particulate matter) AND (extracellular vesicles OR exosomes OR microvesicles OR microparticles OR ectosomes). All original research articles were included and reviewed. Fifty articles were identified, most of which investigated the effect of respiratory exposures on EV release in vitro (25) and/or on circulating EV in human plasma (24). The majority of studies based their main observations on the relatively insensitive scatter-based flow cytometry of EV (29). EV induced by respiratory exposures were found to modulate inflammation (19), thrombosis (13), endothelial dysfunction (11), tissue remodeling (6), and angiogenesis (3). By influencing these processes, EV may play a key role in the development of cardiovascular diseases and chronic obstructive pulmonary disease and possibly lung cancer and allergic asthma. The current findings warrant additional research with improved methodologies to evaluate the contribution of respiratory exposure-induced EV to disease etiology, as well as their potential as biomarkers of exposure or risk and as novel targets for preventive or therapeutic strategies.

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Section: In Vivo Evidence For Tf + Extracellular Vesiclesmentioning

confidence: 92%

Section: In Vivo Evidence For Ps + Extracellular Vesiclesmentioning

confidence: 99%

Section: Conflicting Datamentioning

confidence: 99%

See 1 more Smart Citation

Extracellular vesicles released in response to respiratory exposures: implications for chronic disease

Benedikter

Wouters

Savelkoul

et al. 2018

Journal of Toxicology and Environmental Health, Part B

View full text Add to dashboard Cite

show abstract

“…Other variables tested included measuring neat (no dilution), supernatant and pellets after samples were centrifuged at 21000g for 1 hour. The centrifugation conditions were chosen based on commonly employed methods for isolating EV [12]. The statistical analysis was performed using Prism 6 software (Graphpad, USA).…”

Section: Processing and Analysismentioning

confidence: 99%

“…The relation of size to physiological or pathophysiological role of the EVs is not clear; though it is possible that certain sized biological vesicles have greater capacity for cargo (either RNA or protein) transfer [12,23]. It is important to understand that biological fluids such as plasma are complex and heterogeneous.…”

Section: Concluding Notes On Applications Of Nanotracking and Trps Fomentioning

confidence: 99%

Challenges in Analysis of Circulating Extracellular Vesicles in Human Plasma Using Nanotracking and Tunable Resistive Pulse Sensing

Enjeti¹,

Ariyarajah²,

Warwick³

et al. 2017

J Nanomed Nanotechnol

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Extracellular vesicles in toxicological studies: key roles in communication between environmental stress and adverse outcomes

Qin

Huang

2020

J of Applied Toxicology

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External stressors, especially environmental toxicants can disturb biological homeostasis and thus lead to adverse health effects. However, there is limited understanding of how cells directly exposed to stressors transmit the signals to cells indirectly in contact with stressors. Extracellular vesicles (EVs) are receiving increasing attention as signal transductors between various types of cells in organisms. Cargo in EVs, including RNAs, proteins, lipids, and other signal molecules can be transferred between cells and become critical determining factors of intercellular communication. EVs can be a powerful mediator of environmental stimuli. It has been shown that external stressors reshape the secretion of EVs, modify the composition of EVs, and thus influence the mediating function of EVs. These abnormal EVs can lead to dysfunction of recipient cells, and even the pathogenesis of diseases. In this review, we first summarized current knowledge about the responses of EVs to external stimuli, including chemicals and chemical mixtures. Then we explained how these altered EVs regulate signal pathways in recipient cells, thus mediating physio‐pathological responses in detail. The most up‐to‐date evidence from molecular, cellular, animal and human levels was synthesized to systematically address the mediating roles of EVs. EVs can be regarded as a bridge to link external stressors and internal response. Further toxicological and molecular epidemiological studies are expected to provide further insight into the roles of EVs in toxicology. The gaps in the engulfment of toxicants into EVs are listed as the priority to be solved in future studies.

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Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles

Cited by 36 publications

References 21 publications

Extracellular vesicles released in response to respiratory exposures: implications for chronic disease

Extracellular vesicles released in response to respiratory exposures: implications for chronic disease

Challenges in Analysis of Circulating Extracellular Vesicles in Human Plasma Using Nanotracking and Tunable Resistive Pulse Sensing

Extracellular vesicles in toxicological studies: key roles in communication between environmental stress and adverse outcomes

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