Correlations between lignin content and related genes, weathering deterioration, and soybean seed quality at pre-harvest

Madureira, Alana; Molinari, Mayla Daiane Corrêa; Marin, Silvana Regina Rockenbach; Fuganti-Pagliarini, Renata; Henning, Fernando Augusto; Nepomuceno, Alexandre Lima; Finatto, Taciane; Mertz-Henning, L. M.

doi:10.1007/s12892-023-00193-5

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“…Higher levels of lignin in the seed are associated with higher physiological quality, reduced damage from moisture, less damage caused by storage fungi, and less mechanical damage [ 19 , 69 , 70 ]. Additionally, soybean varieties with black seed coats have better storability due to having smaller pores in their membranes and higher lignin contents [ 20 ]. The cultivar BRSMG 715A has a high lignin content, which is considered one of the determining factors for higher seed quality [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

“…Lignin is also associated with more thickness in other tissues. In pods, higher lignin content is associated with less weathering damage and, consequently, less damage in seeds during maturation and harvesting [ 20 ]. In roots and leaves, lignin plays a role in adaptation against drought, salinity, and temperature variation [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional Profile of Soybean Seeds with Contrasting Seed Coat Color

Kafer

Molinari²,

Henning

et al. 2023

Plants

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Soybean is the primary source of vegetable protein and is used for various purposes, mainly to feed animals. This crop can have diverse seed coat colors, varying from yellow, black, brown, and green to bicolor. Black seed coat cultivars have already been assigned as favorable for both seed and grain production. Thus, this work aimed to identify genes associated with soybean seed quality by comparing the transcriptomes of soybean seeds with contrasting seed coat colors. The results from RNA-seq analyses were validated with real-time PCR using the cultivar BRS 715A (black seed coat) and the cultivars BRS 413 RR and DM 6563 IPRO (yellow seed coat). We found 318 genes differentially expressed in all cultivars (freshly harvested seeds and seeds stored in cold chamber). From the in silico analysis of the transcriptomes, the following genes were selected and validated with RT-qPCR: ACS1, ACSF3, CYP90A1, CYP710A1, HCT, CBL, and SAHH. These genes are genes induced in the black seed coat cultivar and are part of pathways responsible for ethylene, lipid, brassinosteroid, lignin, and sulfur amino acid biosynthesis. The BRSMG 715A gene has almost 4times more lignin than the yellow seed coat cultivars. These attributes are related to the BRSMG 715A cultivar’s higher seed quality, which translates to more longevity and resistance to moisture and mechanical damage. Future silencing studies may evaluate the knockout of these genes to better understand the biology of soybean seeds with black seed coat.

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